Biosensors based on bean sprout homogenate immobilized in chitosan microspheres and silica for determination of chlorogenic acid

Moccelini, Sally Katiuce; Spinelli, Almir; Vieira, Iolanda Cruz

doi:10.1016/j.enzmictec.2008.06.002

Cited by 36 publications

(23 citation statements)

References 33 publications

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“…The determination of CGA or the phenolic compounds have been investigated by using various analytical methods, such as high‐performance liquid chromatography (HPLC) (Fecka and Turek 2008; Liu and others 2008), liquid chromatography–mass spectrometry (Boros and others 2010), liquid chromatography–mass spectrometry/mass spectrometry (Ye and others 2010), capillary zone electrophoresis (Li and others 2010), capillary zone electrophoresis coupled with amperometric detection (Dong and others 2009), capillary zone electrophoresis with electrochemical detection (Tong and others 2009) and biosensor (Mello and others 2003; Carvalho and others 2008; Moccelini and others 2008; Fernandes and others 2009). Electrochemical methods can be used as an alternative method due to its sensitivity, ease to use, low costs, and relatively short analysis times compared with the traditional applied methods.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior of Chlorogenic Acid at a Boron‐Doped Diamond Electrode and Estimation of the Antioxidant Capacity in the Coffee Samples Based on Its Oxidation Peak

Yardım¹

2012

Journal of Food Science

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In this study, an electroanalytical methodology for the determination of chlorogenic acid (CGA) was achieved at a boron-doped diamond electrode under adsorptive transfer stripping voltammetric conditions. The values obtained for CGA were used to estimate the antioxidant properties of the coffee sample based on CGA oxidation. By using square-wave stripping mode, the compound yielded a well-defined voltammetric response at +0.49 V with respect to Ag/AgCl in Britton-Robinson buffer at pH 3.0 (after 120 s accumulations at a fixed potential of 0.40 V). At the optimum experimental conditions, linear calibration curve is obtained within the concentration range of 0.25 to 4.0 μg mL⁻¹ with the limit of detection 0.049 μg mL⁻¹ . The developed protocol was successfully applied for the analysis of antioxidant capacity in the coffee products such as Turkish coffee and instant coffee.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior of Chlorogenic Acid at a Boron‐Doped Diamond Electrode and Estimation of the Antioxidant Capacity in the Coffee Samples Based on Its Oxidation Peak

Yardım¹

2012

Journal of Food Science

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“…The results of the total CQAs content in coffee samples obtained in this study are in agreement with the results of the work of Ziyatdinova et al, [16] who also used the DPV method for determining CGAs in coffee samples, but also with the results of other authors who used the other electrochemical methods or other working electrodes. [11][12][13][14]16,17,31] SW voltammograms of the coffee extracts measured in 0.1 mol L -1 PBS pH 7.0 are shown in Figure 6The potential (Ep ) of the total current response for the coffee extracts has values between 0.223 V and 0.229 V, depending on the type of coffee ( Figure 6). In one of our earlier studies, it was reported that the oxidation potential of CQAs was 0.223 V and 0.229 V for diCQAs.…”

Section: Differential Pulse and Square-wave Voltammetrymentioning

confidence: 99%

“…The most used voltammetric method was square-wave voltammetry (SWV) with different electrochemical sensors or biosensors. [11][12][13] Also, different solid electrodes were C used for analysis. Thus, Yardim [14] , and Yardim et al [15] used adsorptive stripping SWV (AdTSSW) on the boron-doped diamond electrode for determination of CGAs in coffee beverages.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic and Voltammetric Characterization of Chlorogenic Acids in Coffee Samples

2018

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The accurate and precise characterization of eight chlorogenic acids (CGAs) isomers, caffeoylquinic acids (5-CQA, 4-CQA, 3-CQA), dicaffeoylquinic acids (3,5-diCQA, 4,5-diCQA, 3,4-diCQA), and feruloylquinic acids (5-FQA, 4-FQA), was carried out using different brands of coffee. High-performance liquid chromatography (HPLC), differential pulse (DPV) and square-wave voltammetry (SWV) were applied for the investigation. HPLC proved to be an accurate and precise method for separation, identification and quantification of CGAs isomers in coffee extracts. DPV and SWV have shown that electrochemical behaviour of coffee extracts was very similar to that of CGAs isomers and DPV and SWV can be used for the correct characterization of CGAs in coffee. All three techniques have shown a very high correlation of CGAs content in all investigated coffee samples. Therefore, HPLC, DPV and SWV methods can be used in combination as very selective, sensitive and precise methods for characterization of CGAs isomers in coffee extracts.

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“…Mas a utilização de tecidos ou extratos vegetais brutos, além de outras vantagens, faz com que os custos caiam a patamares mínimos, eliminando inclusive a necessidade de gastos com o processo de importação que, além de oneroso, é geralmente lento. Há diversas formas de se aplicar o tecido vegetal na confecção dos biossensores, sendo a utilização de extratos brutos enzimáticos imobilizados, a forma mais citada na literatura (112,113,108,111).…”

Section: Enzimas Fonte Ação Alimentos Aplicação Referênciasunclassified

Enzimas Vegetais: Extração E Aplicações Biotecnológicas

Antunes

Lopes

Brito

et al. 2017

Infarma

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The manuscript describes the current analytical methodologies used in obtaining plant enzymes for biotechnological applications. The isolation and partial purification methods are reported, summarized in chromatographic electrophoretic techniques. The importance of these enzymes in biotechnology research, with a focus on its applications, characteristics and peculiarities, are emphasizing either. With regard to the applicability, the article brings several examples in food production, manufacturing and production of biosensors for determination of various analytes. About bioremediation, we aimed on pollutant removal of the natural environment and its conversion into less harmful products, using those plant enzymes. Industrial interests are also discussed.Keywords: biotechnology; food production; biosensing techniques; bioremediation. RESUMOO artigo descreve as metodologias analíticas atuais mais utilizadas na obtenção de enzimas vegetais para aplicações biotecnológicas. São relatados os métodos de isolamento e purificação parcial, que se resumem nas técnicas cromatográficas e eletroforéticas, enfatizando a importância dessas enzimas nas pesquisas em Biotecnologia, com foco nas suas aplicações, características e peculiaridades. No que se refere à aplicabilidade, o artigo traz diversos exemplos na produção de alimentos, na fabricação e produção de biossenssores para determinação de diversos analitos e na biorremediação, que que tem como objetivo a remoção de poluentes do ambiente natural e a sua conversão em produtos menos prejudiciais, utilizando, para isso, as enzimas vegetais. Interesses industriais também são discutidos.Palavras-chave: produção de alimentos; técnicas biossensoriais; biorremediação.Revisão 10.14450/2318-9312.v29.e3.a2017.pp181-198 INTRODUÇÃO A Biotecnologia é o conjunto de técnicas que permitem gerar produtos de interesse econômico e social a partir de organismos vivos ou de seus componentes (1). Nas últimas décadas apresentou uma expansão de aplicações bem sucedidas em produtos e técnicas, principalmente aquelas envolvendo enzimas (2). As enzimas são consideradas catalisadores biológicos, por exercerem a função de acelerar ou mesmo possibilitar reações entre componentes químicos (3). Estão presentes em todos os sistemas biológicos, são produzidas por todos os organismos vivos e são responsáveis por vários processos metabólicos (4).As enzimas são o principal alvo da pesquisa em Biotecnologia, não apenas por seu papel crucial nos me-

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Biosensors based on bean sprout homogenate immobilized in chitosan microspheres and silica for determination of chlorogenic acid

Cited by 36 publications

References 33 publications

Electrochemical Behavior of Chlorogenic Acid at a Boron‐Doped Diamond Electrode and Estimation of the Antioxidant Capacity in the Coffee Samples Based on Its Oxidation Peak

Electrochemical Behavior of Chlorogenic Acid at a Boron‐Doped Diamond Electrode and Estimation of the Antioxidant Capacity in the Coffee Samples Based on Its Oxidation Peak

Chromatographic and Voltammetric Characterization of Chlorogenic Acids in Coffee Samples

Enzimas Vegetais: Extração E Aplicações Biotecnológicas

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