Biópsia hepática por laparotomia paracostal em bovinos e búfalos

Minervino, Antônio Humberto Hamad; Júnior, Raimundo Alves Barrêto; Rodrigues, Frederico Augusto Mazzocca Lopes; Ferreira, Rodrigo Nogueira Fernandes; Saut, João Paulo Elsen; Queiróz, Genílson Fernandes de; Reis, Leonardo Frasson dos; Ortolani, Enrico Lippi

doi:10.1590/s0103-84782009000300025

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…The blood was collected by jugular venipuncture using vacuum tubes without anticoagulant, which were centrifuged at 1032× g for 5 min to obtain serum, which was stored in plastic microtubes at −20 °C for subsequent analysis. Liver biopsy was performed as described by Minervino et al [ 9 ] and bone biopsy (rib) according to the classic methodology [ 10 ]. The samples were stored in sterile cryovials and frozen at −20 °C for subsequent analysis.…”

Section: Methodsmentioning

confidence: 99%

Seasonality Effects on the Mineral Profile of Goats Farmed in the Semiarid Region of Brazil

Lima

Vale

Sousa

et al. 2021

Veterinary Sciences

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Seasonality effects on the mineral profile of goats were evaluated. Fifty males were divided into two groups, one with mineral supplementation and one control. Seasonal evaluation was conducted during four periods: beginning, middle, and end of the dry period and middle of the rainy period. Rib and liver biopsies were performed, and blood was collected at each period to evaluate mineral accumulation. Ca, P, Cu, Fe, Mo, Zn, and Co concentrations were determined using inductively coupled plasma optical emission spectrometry after acid digestion. Normal Ca, P, and Mo; low Cu, Zn, and Co; and high Fe levels were observed in the diet. The young animals analyzed showed normal serum and bone Ca and P concentrations, suggesting no need for supplementation throughout the entire year under the conditions of this study. Iron showed high values throughout the year, being potentially dangerous especially owing to its antagonistic relationship with other elements. Cu and Zn deficiency in the diet was observed under the conditions of this study, requiring supplementation with values higher than those contained in the mineral supplement used in the middle and end of the dry period. The supply of specific mineral supplement formulated for animals farmed in the semiarid region is suggested and would reduce costs.

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Section: Methodsmentioning

confidence: 99%

Seasonality Effects on the Mineral Profile of Goats Farmed in the Semiarid Region of Brazil

Lima

Vale

Sousa

et al. 2021

Veterinary Sciences

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show abstract

“…Blood samples were obtained at the beginning and the end of the study, by jugular venepuncture, and were stored in vacuum tubes with lithium heparin. Liver biopsies were performed on day 30 of the adaptation period using a surgical technique described by Minervino [31] and at the end of the study after slaughter ( postmortem biopsy). Bile samples were collected at the same time.…”

Section: Methodsmentioning

confidence: 99%

Dietary Zinc Supplementation to Prevent Chronic Copper Poisoning in Sheep

Minervino

López‐Alonso

Júnior

et al. 2018

Animals

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Simple SummarySheep are susceptible to copper toxicosis, a deadly disease that usually occurs when the animals ingest large amounts of this mineral. Considering that the susceptibility of sheep to copper accumulation varies widely among breeds and from animal to animal., we evaluate whether Zn supplementation could be an option as a preventive measure to protect against hepatic Cu accumulation in sheep. Zn at 300 mg/kg dry matter (DM) is useful for preventing excessive hepatic Cu accumulation. Hepatic Cu accumulation is lower in animals receiving the Zn supplementation.AbstractThe aim of this study was to evaluate whether zinc (Zn) supplementation protects against hepatic copper (Cu) accumulation in copper-loaded sheep. Forty cross-bred lambs were assigned to five experimental groups. These included the control group (C) and four treatment groups that received Cu and/or Zn supplementation (dry matter (DM) basis) over 14 weeks, as follows: Cu (450 mg Cu/kg); Zn-35 (450 mg Cu + 35 mg Zn/kg); Zn-150 (450 mg Cu + 150 mg Zn/kg); and Zn-300 (450 mg Cu + 300 mg Zn/kg). Blood, liver, and bile samples were obtained for mineral determination by inductively coupled plasma optical emission spectrometry (ICP–OES). The hepatic metallothionein (MT) concentrations were also determined. At the end of the experiment, hepatic Cu concentrations were higher in all Cu-supplemented groups than in C. Hepatic Cu accumulation was lower in the groups receiving the Zn supplementation than in the Cu group, although the difference was only statistically significant (66%) in the Zn-300 group. The MT concentrations tended to be higher (almost two-fold) in the Zn groups (but were not dose related) than in the C and Cu groups, and they were related to hepatic Zn concentrations. Zn supplementation at 300 mg/kg DM is useful for preventing excessive hepatic Cu accumulation in sheep exposed to high dietary concentrations of Cu.

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Effects of Sodium Monensin on Copper Metabolism of Brazilian Santa Inês Sheep Submitted to Different Dietary Copper

Rodrigues

Sousa

Minervino

et al. 2022

Biol Trace Elem Res

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Biópsia hepática por laparotomia paracostal em bovinos e búfalos

Abstract: A técnica de biópsia hepática em ruminantes tem importante valor no diagnóstico clínico de doenças tóxicas e metabólicas, em especial nos desequilíbrios minerais. As técnicas mais comumente utilizadas restringem análises devido

Cited by 6 publications

References 5 publications

Seasonality Effects on the Mineral Profile of Goats Farmed in the Semiarid Region of Brazil

Seasonality Effects on the Mineral Profile of Goats Farmed in the Semiarid Region of Brazil

Dietary Zinc Supplementation to Prevent Chronic Copper Poisoning in Sheep

Effects of Sodium Monensin on Copper Metabolism of Brazilian Santa Inês Sheep Submitted to Different Dietary Copper

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