Bioproduction of<scp>D</scp>-Psicose from Allitol with<i>Enterobacter aerogenes</i>IK7: A New Frontier in Rare Ketose Production

Gullapalli, Pushpakiran; Takata, Goro; Poonperm, Wayoon; Rao, Dingqi; Morimoto, Kenji; Akimitsu, Kazuya; Tajima, Shigeyuki; Izumori, Ken

doi:10.1271/bbb.70450

Cited by 13 publications

(13 citation statements)

References 29 publications

(35 reference statements)

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“…According to Gullapalli et al ( 2007), the type of medium used in fermentation greatly influences the type and quantity of products formed. Choi et al ( 2011) found that yeast extract was best for biomass accumulation and ethanol yield by K. cryocrescens S26.…”

Section: Discussionmentioning

confidence: 99%

“…Choi et al ( 2011) found that yeast extract was best for biomass accumulation and ethanol yield by K. cryocrescens S26. However, Gullapalli et al ( 2007), in their bioproduction of D-psicose by E. aerogenes , discovered that the highest transformation rate was obtained when tryptic soy broth (TSB) was the fermentation broth. In a preliminary study, Banna and Ouro (2008, unpublished) used Luria Bertani (LB) broth supplemented with tryptone and yeast extract as fermentation medium for E. aerogenes .…”

Section: Discussionmentioning

confidence: 99%

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Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012

et al. 2013

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The aim of this research is to optimize the cultural conditions for the conversion of glycerol to ethanol by Enterobacter aerogenes S012. Taguchi method was used to screen the cultural conditions based on their signal to noise ratio (SN). Temperature (°C), agitation speed (rpm) and time (h) were found to have the highest influence on both glycerol utilization and ethanol production by the organism while pH had the lowest. Full factorial design, statistical analysis, and regression model equation were used to optimize the selected cultural parameters for maximum ethanol production. The result showed that fermentation at 38°C and 200 rpm for 48 h would be ideal for the bacteria to produce maximum amount of ethanol from glycerol. At these optimum conditions, ethanol production, yield and productivity were 25.4 g/l, 0.53 g/l/h, and 1.12 mol/mol-glycerol, repectively. Ethanol production increased to 26.5 g/l while yield and productivity decreased to 1.04 mol/mol-glycerol and 0.37 g/l/h, respectively, after 72 h. Analysis of the fermentation products was performed using HPLC, while anaerobic condition was created by purging the fermentation vessel with nitrogen gas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012

et al. 2013

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“… and Gullapalli et al . reported that Bacillus pallidus Y25 and Enterobacter aerogenes IK7 showed the broad substrate specificity to oxidize various polyols to ketoses, especially allitol to D ‐psicose, but they did not mention the reduction of allitol from D ‐psicose. From the above, the dehydrogenase from K. oxytoca G4A4 may be a novel ribitol dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

“…The cells were grown on the selected carbon source at concentrations ranging from 0.05 to 0.5%. The conditions of the standard reaction mixture were modified by different temperatures (20,30,37, and 50°C), cell densities (20, 40, and 60; OD 600 nm ), and reaction buffers (0.2 M acetate buffer pH 4.0 to pH 6.0, 0.2 M phosphate buffer pH 6.0 to pH 8.0, 50 mM Tris-HCl buffer pH 8.0-9.0, and 50 mM glycine-NaOH buffer pH 9.0-11.0). The transformation rate at different substrate concentrations (0.25, 0.5, and 1% D-psicose) was detected at different time points.…”

Section: Optimal Cultivation Conditions For Allitol Transformationmentioning

confidence: 99%

Production of allitol from D‐psicose by a novel isolated strain of Klebsiella oxytoca G4A4

Han

Zhu

Men

et al. 2014

J. Basic Microbiol.

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A novel bacterium capable of producing allitol from D-psicose was isolated from soil and identified as Klebsiella oxytoca G4A4. An efficient method for the transformation of D-psicose to allitol was achieved through the resting cell reaction. Ribitol as an inducer is suitable for cell cultivation, and cells are most active in Tris-HCl buffer (pH 8.0) at 37 °C with a density of 40 (OD600 nm ). After the reaction, the final conversion rates of the washed cells were approximately 87, 83, and 55% at D-psicose concentrations of 0.25, 0.5, and 1%, respectively. The product from D-psicose was purified and determined to be allitol by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy.

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“…The reaction of allitol with fuming hydrochloric acid at 100°C was used for the synthesis of 1,4-anhydro-5,6-dichloro-5,6-dideoxy-DL-talitol and 1,4-anhydro-6chloro-6-deoxy-DL-allitol (Ballard & Stacey, 1973). Gullapalli et al (2007) reported production of D-psicose from allitol using the novel strain Enterobacter aerogenes IK7; 10% of the allitol was transformed into D-psicose, which suggests this is a suitable method for commercial production. Allitol exists in nature in Itea plants and can undergo biotransformation into D-psicose and L-psicose.…”

Section: Application Of Allitolmentioning

confidence: 99%

Allitol: production, properties and applications

Hassanin

Koko

et al. 2016

Int J of Food Sci Tech

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Summary Allitol is a rare alcohol monosaccharide sweetener containing six carbons atoms. It is a major product of the d‐psicose reduction pathway that exerts various physiological effects, for example laxative effects for the treatment of constipation and anti‐obesity effects by suppressing lipid accumulation. Allitol can be also used as an anticrystallisation agent. In addition, allitol cross‐links d‐ and l‐hexoses, thus contributing to production of l‐psicose. This is a review of the recent studies on the sources, properties, physiological functions and applications of allitol. In addition, the biochemical properties of ribitol dehydrogenase enzymes and the biotechnological production of allitol via microbial and enzymatic synthesis from d‐psicose and directly from d‐fructose are reviewed.

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Bioproduction ofD-Psicose from Allitol withEnterobacter aerogenesIK7: A New Frontier in Rare Ketose Production

Cited by 13 publications

References 29 publications

Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012

Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012

Production of allitol from D‐psicose by a novel isolated strain of Klebsiella oxytoca G4A4

Allitol: production, properties and applications

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