Bioprintable Alginate/Gelatin Hydrogel 3D &lt;em&gt;In Vitro&lt;/em&gt; Model Systems Induce Cell Spheroid Formation

Jiang, Tao; Munguia-Lopez, Jose G.; Flores-Torres, Salvador; Grant, Joel; Vijayakumar, Sekar; León‐Rodríguez, Antonio De; Kinsella, Joseph M.

doi:10.3791/57826-v

Cited by 7 publications

(8 citation statements)

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“…To select the optimum G-SA hydrogel among the different formulations to be used as the 3D culture system, a preliminary comparative assay on cytocompatibility was performed and nally, the hydrogel with 5w% gelatin and 5w% sodium alginate was chosen because a higher proliferation rate was observed meaning that this hydrogel provided the superior environment for GBM cells. Gelatin and sodium alginate were previously used as 3D in vitro model systems to induce the formation of breast cancer cell spheroid through bioprinting 17,18 . The authors in both studies found that the bio-printed heterogeneous model could result in high rate of viability and self-assembly of breast cancer cells to form multicellular tumor spheroids with the cancer-associated broblast cells migration and interaction.…”

Section: Discussionmentioning

confidence: 99%

“…Hydrogels based on sodium alginate (SA) (a natural polysaccharide) are attractive biocompatible materials with the capability of cell encapsulation and drug delivery, that are widely used in the 3D culture of cancerous cells [16][17][18] . The alginate gelation and cross-linking can be accomplished by the sodium ions exchange with multivalent cations such as Ca 2+ , for example by use of CaCl 2 19 .…”

Section: Introductionmentioning

confidence: 99%

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Development of a hydrogel-based three Dimensional (3D) Glioblastoma Cell lines Culture as a Model System for CD73 inhibitor response study

Bahraminasab

Asgharzade

Doostmohamadi

et al. 2023

Preprint

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Despite the development of various therapeutic approaches over the past decades, the glioblastoma (GBM) treatment remains a major challenge. The extracellular adenosine-generating enzyme CD73 is involved in the pathogenesis and progression of GBM, and targeting CD73 may represent a novel approach to this cancer. This study characterized three-dimensional culture systems based on three compositions of hydrogel and chose an optimum type for local delivery of CD73 to target GBM cells as a possible therapeutic approach for this disease. Rheology measurements, Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and cell proliferation assay were conducted to analyze the synthesized hydrogel and choose an optimal formula. The viability of tumor cells in the optimal hydrogel was assessed by histology and confocal microscopy imaging. Furthermore, tumor cells' sensitivity to CD73 inhibitor was investigated by cell proliferation assay and real-time PCR. The data demonstrated that the hydrogel with 5 w% gelatin and 5 w% sodium alginate had superior rheological properties and cell viability. Therefore, it could provide a more suitable environment for GBM cells and mimic the natural microenvironment more properly. CD73 inhibitor-treated GBM cells significantly decreased proliferation rate and expressions of VEGF and HIF1-α within the optimal hydrogel. Our current research revealed the great potential of CD73 inhibitor for clinical translation of cancer study by analyzing 3D tumor cell behavior and function, and therefore for more effective treatment protocols for GBM.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a hydrogel-based three Dimensional (3D) Glioblastoma Cell lines Culture as a Model System for CD73 inhibitor response study

Bahraminasab

Asgharzade

Doostmohamadi

et al. 2023

Preprint

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“…The mechanical pressure of extrusion printers can also damage cultured cells. Jiang ( 117 ) et al. combined alginate and gelatin to form a composite hydrogel similar to a natural tumor matrix.…”

Section: D Model Construction Methods For Cancer Research and Its Rec...mentioning

confidence: 99%

Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment

et al. 2023

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Three-dimensional cell culture technology (3DCC) sits between two-dimensional cell culture (2DCC) and animal models and is widely used in oncology research. Compared to 2DCC, 3DCC allows cells to grow in a three-dimensional space, better simulating the in vivo growth environment of tumors, including hypoxia, nutrient concentration gradients, micro angiogenesis mimicism, and the interaction between tumor cells and the tumor microenvironment matrix. 3DCC has unparalleled advantages when compared to animal models, being more controllable, operable, and convenient. This review summarizes the comparison between 2DCC and 3DCC, as well as recent advances in different methods to obtain 3D models and their respective advantages and disadvantages.

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“…To construct a 3D tumor model that mimics the TME, Jiang et al 88 adopted extrusion 3D bioprinting to embed breast cancer cells and cancer-associated fibroblasts (CAFs) in a gelatin–alginate matrix hydrogel. The CAFs were then used to print propeller-like structures that encircled the tumor cells (Fig.…”

Section: Tumor Microenvironmentmentioning

confidence: 99%

“…Genes linked to the development of tumor were upregulated with the addition of fibroblasts, indi-cating that multicellular co-culture is essential and important to the TME. 87 To construct a 3D tumor model that mimics the TME, Jiang et al 88 adopted extrusion 3D bioprinting to embed breast cancer cells and cancer-associated fibroblasts (CAFs) in a gelatin-alginate matrix hydrogel. The CAFs were then used to print propeller-like structures that encircled the tumor cells (Fig.…”

Section: Reviewmentioning

confidence: 99%

3D bioprinting tumor models mimic the tumor microenvironment for drug screening

Yue

et al. 2023

Biomater. Sci.

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Bioprintable Alginate/Gelatin Hydrogel 3D <em>In Vitro</em> Model Systems Induce Cell Spheroid Formation

Cited by 7 publications

References 0 publications

Development of a hydrogel-based three Dimensional (3D) Glioblastoma Cell lines Culture as a Model System for CD73 inhibitor response study

Development of a hydrogel-based three Dimensional (3D) Glioblastoma Cell lines Culture as a Model System for CD73 inhibitor response study

Towards a New 3Rs Era in the construction of 3D cell culture models simulating tumor microenvironment

3D bioprinting tumor models mimic the tumor microenvironment for drug screening

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