3D bioprinting tumor models mimic the tumor microenvironment for drug screening

Abstract

This review describes 3D bioprinting methods, the use of bioinks in tumor models, and in vitro tumor model design strategies for building complex tumor microenvironment features using biological 3D printing technology.

The future of cancer therapy: exploring the potential of patient-derived organoids in drug development

“…The ongoing research is improving the fidelity of PDOs used in cancer research by addressing their limitations in simulating the TME. Bioprinting technologies show promise in reconstructing the TME with greater accuracy, leading to more representative organoid models ( Mi et al, 2023 ). Researchers are developing advanced culture media that better support PDO viability by mimicking interactions with stromal cells and key signaling molecules ( Manduca et al, 2023 ).…”

Embedded bioprinted multicellular spheroids modeling pancreatic cancer bioarchitecture towards advanced drug therapy

“…Beyond these, 3D bioprinting techniques, such as inkjet bioprinting, 15 extrusion bioprinting 16 and light-assisted bioprinting, 17,18 have emerged as advanced biomanufacturing technologies that allow cell-laden hydrogels to be positioned into specific structures in a programmed manner, and have been widely applied for the construction of 3D tumor models. [19][20][21] A laser-assisted bioprinting process was adopted to generate pancreas spheroid models for studying cancer initiation. 22 Additionally, our previous work reported a heterogeneous PDAC microtissue that was fabricated by printing cellladen hydrogel beads for use as a drug screening platform.…”

Toward morphologically relevant extracellular matrix: nanofiber-hydrogel composites for tumor cell culture

“…Reliable in vitro cancer models are often considered powerful tools for studying the mechanisms of cancer development, assessing the efficacy of new drugs and understanding potential approaches to cancer treatment. 1,2 The demand for three-dimensional (3D) in vitro models is steadily increasing, driven by the necessity to capture the intricate complexity of tumor biology and drug development more effectively compared to traditional two-dimensional (2D) cultures. 2,3 3D culture systems provide a more realistic biochemical and biomechanical microenvironment, offering not only a comprehensive overview of tumor cell signaling in vivo but also enabling faithful reproduction of human tumor cell-matrix interactions in vitro .…”