Biopolymer-Based Hydrogels As Scaffolds for Tissue Engineering Applications: A Review

Vlierberghe, Sandra Van; Dubruel, Peter; Schacht, Etienne

doi:10.1021/bm200083n

Cited by 1,527 publications

(1,080 citation statements)

References 320 publications

(440 reference statements)

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“…Hydrogels are hydrophilic, physically or chemically cross-linked polymeric structures, which are particularly attractive for a range of biomedical applications including, for example, drug delivery 1 and tissue engineering 2 . This interest stems from a combination of useful properties such as antifouling behaviour, tunable mechanical properties, the many routes available for selfassembly, the ease by which biomolecules may be incorporated, and the ability to control swelling or phase transitions by external stimuli, and thereby also diffusion into or out of the polymer network.…”

Section: Introductionmentioning

confidence: 99%

pH-control of the protein resistance of thin hydrogel gradient films

et al. 2014

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We report on the preparation and characterization of thin polyampholytic hydrogel gradient films permitting pH-controlled protein resistance via the regulation of surface charges. The hydrogel gradients are composed of cationic poly(2-aminoethyl methacrylate hydrochloride) (PAEMA), and anionic poly(2-carboxyethyl acrylate) (PCEA) layers, which are fabricated by Self-Initiated Photografting and Photopolymerization (SIPGP). Using a two-step UV exposure procedure, a polymer thickness gradient of one component is formed on top of a uniform layer of the oppositely charged polymer. The swelling of the gradient films in water and buffers at different pH were characterized by imaging spectroscopic ellipsometry. The surface charge distribution and steric interactions with the hydrogel gradients were recorded by direct force measurement with colloidal-probe atomic force microscopy. We demonstrate that formation of a charged polymer thickness gradient on top of a uniform layer of opposite charge can result in a region of charge-neutrality. This charge-neutral region is highly resistant to non-specific adsorption of proteins, and its location along the gradient can be controlled via the pH of the surrounding buffer. The pH-controlled protein adsorption and desorption was monitored in real-time by imaging surface plasmon resonance, while the corresponding redistribution of surface charge was confirmed by direct force measurements.

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Section: Introductionmentioning

confidence: 99%

pH-control of the protein resistance of thin hydrogel gradient films

et al. 2014

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“…Therefore, hydrogels are of interest for many biomedical applications such as tissue engineering scaffolds, controlled drug release devices, and biosensors [2]. Hydrogels can be made of either synthetic materials or naturally derived materials [3]. Among naturally derived materials, it is agreed that silkworm silk fibroin (SF) is one of the most promising biomaterials because of its excellent biocompatibility, biosafety, controllable biodegradation rates, processability, and mechanical properties [4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao

Xiong

et al. 2012

Advances in Materials Science and Engineering

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Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of the Antheraea pernyi (A. pernyi) SF solution was significantly shorter than that of the Bombyx mori (B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins of A. pernyi and B. mori. The gelation time of both A. pernyi SF and B. mori SF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation of β-sheets.

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“…Hydrogels have been widely used to assist the regenerative and reparative processes of many tissues [41,198,199] and several studies were performed assessing their potential as wound healing adjuvant, mainly due to their porosity, permeability, viscoelastic properties and low immunogenicity (depending on the source) [21,39,64,114]. Hydrogels are defined as crosslinked 3D network structures obtained from a range of synthetic or natural polymers that can absorb and retain large amounts of water [7,168].…”

Section: Hydrogel Bioinksmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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Biopolymer-Based Hydrogels As Scaffolds for Tissue Engineering Applications: A Review

Cited by 1,527 publications

References 320 publications

pH-control of the protein resistance of thin hydrogel gradient films

pH-control of the protein resistance of thin hydrogel gradient films

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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