Biopolymer-based films and membranes as wound dressings

Souza, Renata Francielle Bombaldi de; Souza, Fernanda Carla Bombaldi de; Bierhalz, Andréa Cristiane Krause; Pires, Ana Luíza Resende; Moraes, Ângela Maria

doi:10.1016/b978-0-12-818134-8.00007-9

Cited by 14 publications

(6 citation statements)

References 126 publications

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“…However, several reports in the literature support the positive action of free chitosan or combined with other formulations in tissue repair, such as particles, sponges, hydrogels, or films. Similar observations are reported for PEG [ 3 , 46 ].…”

Section: Discussionsupporting

confidence: 89%

“…For this reason, the search for new therapeutic strategies to promote a faster healing of lesions is a worldwide challenge. For this to be possible, these dressings must meet some basic principles, such as being hypoallergenic and biocompatible, providing moisture maintenance at appropriate limits, allowing gas exchange, providing thermal control, being flexible and able to adapt to the injury site, favoring cell proliferation, and protecting the injured area from contaminants, among other characteristics [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Chitosan can be obtained from the deacetylation of chitin, which is found in the exoskeleton of crustaceans, insects, and mollusks and in the cell wall of fungi [ 6 , 7 ]. Through deacetylation, it is possible to increase the solubility of the natural polysaccharide and its technological applicability [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

et al. 2020

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Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The objective of this study was to evaluate the effects of the use of electrospun fibrous membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm2 lesion was made on the animals’ backs, and the membranes were sutured in place. The tissues were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were analyzed by histology with Hematoxylin and Eosin (H&E) and Sirius Red stains, morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, greater expression of proliferating cell nuclear antigen (PCNA), and smooth muscle actin (α-SMA) (p < 0.05) compared to the PCL group. On the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-α) and PCNA (p < 0.05). In addition, it showed a greater immunolabeling of Monocyte Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to the PCL group. The PCL+CHI/PEO membrane modulated the increase in the inflammatory infiltrate, the expression of MCP-1, PCNA, and α-SMA in lesions of mice.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

et al. 2020

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“…However, with the recent development of biomedical technologies, the greatest attention is paid to the natural macromolecules in this field [ 4 , 5 , 6 ]. For example, they are used as components in drug delivery systems [ 7 , 8 ] or wound dressing [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Graft Copolymers with N-Vinylimidazole as Promising Matrices for Immobilization of Bromelain, Ficin, and Papain

et al. 2022

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This work aims to synthesize graft copolymers of chitosan and N-vinylimidazole (VI) with different compositions to be used as matrices for the immobilization of cysteine proteases—bromelain, ficin, and papain. The copolymers are synthesized by free radical solution copolymerization with a potassium persulfate-sodium metabisulfite blend initiator. The copolymers have a relatively high frequency of grafting and yields. All the synthesized graft copolymers are water-soluble, and their solutions are characterized by DLS and laser Doppler microelectrophoresis. The copolymers are self-assembled in aqueous solutions, and they have a cationic nature and pH-sensitivity correlating to the VI content. The FTIR data demonstrate that synthesized graft copolymers conjugate cysteine proteases. The synthesized copolymer adsorbs more enzyme macromolecules compared to non-modified chitosan with the same molecular weight. The proteolytic activity of the immobilized enzymes is increased up to 100% compared to native ones. The immobilized ficin retains up to 97% of the initial activity after a one-day incubation, the immobilized bromelain retains 69% of activity after a 3-day incubation, and the immobilized papain retains 57% of the initial activity after a 7-day incubation. Therefore, the synthesized copolymers can be used as matrices for the immobilization of bromelain, ficin, and papain.

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“…Thus, films were realized using two biopolymers: xanthan gum and sodium alginate, FDA approved as G.R.A.S. (generally recognized as safe) [ 13 , 14 , 15 , 16 ]. The study was divided in three steps: (i) identification of the best film composition and preparation method, (ii) investigation of unloaded film characteristics, and (iii) PYC loading and study of the loaded film performances.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Xanthan Gum and Alginate Based Bioadhesive Film for Pycnogenol Topical Use in Wound Treatment

et al. 2021

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Pycnogenol (PYC) is a concentrate of phenolic compounds derived from French maritime pine; its biological activity as antioxidant, anti-inflammatory and antibacterial suggests its use in the treatment of open wounds. A bioadhesive film, loaded with PYC, was prepared by casting, starting with a combination of two biopolymer acqueous solutions: xanthan gum (1% wt/wt) and sodium alginate (1.5% wt/wt), in a 2.5/7.5 (wt/wt) ratio. In both solutions, glycerol (10% wt/wt) was added as plasticizing agent. The film resulted in an adhesive capable to absorb a simulated wound fluid (~65% wt/wt within 1 h), therefore suitable for exuding wounds. The mechanical characterization showed that the film is deformable (elastic modulus E = 3.070 ± 0.044 MPa), suggesting adaptability to any type of surface and resistance to mechanical solicitations. PYC is released within 24 h by a sustained mechanism, achieving a maximum concentration of ~ 0.2 mg/mL, that is safe for keratinocytes, as shown by cytotoxicity studies. A concentration of 0.015 mg/mL is reached in the first 5 min after application, at which point PYC stimulates keratinocyte growth. These preliminary results suggest the use of PYC in formulations designed for topical use.

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Biopolymer-based films and membranes as wound dressings

Cited by 14 publications

References 126 publications

Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

Chitosan Graft Copolymers with N-Vinylimidazole as Promising Matrices for Immobilization of Bromelain, Ficin, and Papain

Development and Characterization of Xanthan Gum and Alginate Based Bioadhesive Film for Pycnogenol Topical Use in Wound Treatment

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