Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

Zanchetta, Flávia Cristina; Trinca, Rafael Bergamo; Silva, Juliany Lino Gomes; Breder, Jéssica da Silva Cunha; Cantarutti, Thiago Anselmo; Consonni, Sílvio Roberto; Moraes, Ângela Maria; Araújo, Eliana P.; Saad, Mário José Abdalla; Adams, Gary G.; Lima, Maria

doi:10.3390/polym12071580

Cited by 10 publications

(3 citation statements)

References 55 publications

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“…Maksym et al mixed polyethylene oxide (PEO) with CS (3:1) and dissolved it in an acetic acid solution (Deineka et al, 2021). PEO has good biocompatibility and can synthesize polymers in water (Zanchetta et al, 2020). The advantage of the electroviscosity sponge is that it can act as a hemostatic sponge with higher porosity (average pore area is 164 µm 2 ), which can accelerate the absorption of fluid around the wound.…”

Section: Spongementioning

confidence: 99%

Application of chitosan-based materials in surgical or postoperative hemostasis

et al. 2022

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Uncontrolled bleeding from trauma or surgery remains an important factor affecting the survival and prognosis of surgical patients. Failure to timeously stop bleeding will not only prolong the operative time but also threaten the patient’s life. Timely hemostasis after bleeding has become the most concerned event for surgeons. At present, the most commonly used hemostasis methods in the operating room include ligation of blood vessels, electrocautery, and gauze compression to stop bleeding. However, These hemostatic methods do great harm to surrounding tissues while achieving hemostasis. Based on tissue engineering repair strategies, the use of natural polymer materials as hemostatic agents has achieved clinical success. Gelatin sponge and cellulose gauze have been used clinically with good results. However, gelatin sponges are very expensive and place enormous financial pressure on patients. Therefore, there is an urgent need for new hemostatic materials for surgical hemostasis. Chitosan is a natural polysaccharide with biocompatibility and biodegradability, which plays an important role in tissue engineering and regenerative medicine. Chitosan gauze has been proven to have good hemostatic effects. The positive charge on the surface of chitosan can adsorb red blood cells and platelets at the bleeding site to form platelet thrombosis. However, chitosan is not easily soluble in water and has poor adsorption, which makes it a weak local hemostatic agent. Therefore, it is important to improve chitosan-based hemostatic material such that it l has an excellent hemostatic effect. In this review, we introduce the physiological coagulation process and discuss the physicochemical properties of chitosan and its role in hemostasis. Furthermore, we discuss the advantages and disadvantages of chitosan-based hemostatic materials. Finally, we summarize and discuss chitosan-based hemostatic materials.

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Section: Spongementioning

confidence: 99%

Application of chitosan-based materials in surgical or postoperative hemostasis

et al. 2022

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“…Furthermore, the composition of hydrogen bonds formed after chitosan dissolves in an acid solution complicates the spinning process, limiting the use of pure chitosan [40].Extra added polymers, like caprolactone (PCL), polyvinyl alcohol (PVA), or polyethylene oxide (PEO), are now used to improve chitosan spinning, reduce conductivity, or produce thinner bers [41]. The best way to stabilize the chitosan electroprocessing route would have been to add PEO, a watersoluble synthetic polymer with good biocompatibility and low toxicity [42]. In a previous study, PEO was used, which binds to chitosan via hydrogen bonds, lowering the viscosity and interfacial of the chitosan solution.…”

Section: Introductionmentioning

confidence: 99%

Novel Platform Assays for the Diagnosis of Hemostatic Disorders: A Systematic Review

Alzahrani

2022

Preprint

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Background In hemostasis research the novel platform assays for the diagnosis of hemostatic disorders were limited. The purpose of the present review study would be to make a comparison between several assays for assessing the novel hemostatic techniques used in the diagnosis of coagulation disturbance and to point out the most advantageous and disadvantageous aspects of each method as a predictor of morbidity and mortality to determine hemostatic efficacy as well as biological safety. Methods The current systematic review follows the guidelines for observational studies in epidemiology and the PRISMA guidelines. The keywords were used to search the electronic databases (PubMed) (a novel platform to evaluate hemostasis). Searches were restricted to articles published between December 2016 and December 2021; original articles were written in English. To summarize, we collected bibliographies of abstract articles published on a novel platform to evaluate hemostasis studies, Results Following the removal of duplicates, articles were determined by examining the titles and abstracts. Disagreements were resolved through consensus and the application of novel hemostatic analysis methods. The researchers independently reviewed the relevant studies of the recognized records (n = 503), excluding duplicates (n = 9) and irrelevant studies (249). The remaining 254 studies were read in their entirety, and the data from the seven included studies had been extracted Conclusion Nanotechnology-Based Published study formulations discovered it is more optimal for some lab tests when expressed as an anticoagulant for the in vivo evaluation of nanoparticle implications on the complement system, and flow cytometer evaluation could be a promising platform approach for use in hemostasis managers.

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“…To improve the spinning of chitosan, reduce electrical conductivity and obtain thinner fibers, additional polymers, such as caprolactone (PCL), polyvinyl alcohol (PVA) or polyethylene oxide (PEO), are used [ 17 ]. We assumed that the best solution to stabilize the chitosan electrospinning process would be the addition of PEO as a water-soluble synthetic polymer with good biocompatibility and low toxicity [ 18 ]. In this study, we used PEO, which binds to chitosan due to hydrogen bonds, reducing the chitosan solution’s viscosity, surface tension and conductivity, which makes it possible to obtain a dense network of nanofibers [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials

et al. 2021

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The application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEO-copolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.

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Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

Cited by 10 publications

References 55 publications

Application of chitosan-based materials in surgical or postoperative hemostasis

Application of chitosan-based materials in surgical or postoperative hemostasis

Novel Platform Assays for the Diagnosis of Hemostatic Disorders: A Systematic Review

Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials

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