Biophysical and biochemical properties of a binary lipid mixture for DNA transfection

Mirska, D.; Schirmer, Katharina; Funari, Sérgio S.; Langner, Andreas; Dobner, Bodo; Brezesinski, Gerald

doi:10.1016/j.colsurfb.2004.10.007

Cited by 68 publications

(63 citation statements)

References 57 publications

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“…On the other hand, the large amount of CTAB dispersed in solution interferes with biological processes and engenders significant cytotoxicity. [5,11,12] This toxicity is not due to the CTAB layers that are tightly bound to nanoparticles in human cells, as discussed in detail by Wyatt and co-workers. [5] Instead, these researchers have shown that it is the unbound CTAB that is toxic to the cultivated cells.…”

Section: Introductionmentioning

confidence: 78%

Synthesis, Assembly, and Biofunctionalization of Silica-Coated Gold Nanorods for Colorimetric Biosensing

et al. 2006

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Section: Introductionmentioning

confidence: 78%

Synthesis, Assembly, and Biofunctionalization of Silica-Coated Gold Nanorods for Colorimetric Biosensing

et al. 2006

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“…[10][11][12][13][14][15] If a new lipid with promising transfection activity and low toxicity is identified, the next step is to elucidate its structurefunction relationship. [16,17] Investigation of the physicochemical properties turned out to be a domain that can correlate structural characteristics with transfection results. [18] Cationic liposome/DNA complexes can be used as nonviral vectors for direct delivery of DNA-based biopharmaceuticals to damaged cells and tissues.…”

Section: Introductionmentioning

confidence: 99%

“…This behavior was expected and has been shown before. [33] McMullen et al demonstrated that in mixtures of cholesterol and phoshatidylcholines with a hydrocarbon chain length of C 16 T m increases, while in mixtures with longer chain phoshatidylcholines (chain length ! C 18 ) T m decreases.…”

mentioning

confidence: 97%

Structure–Function Relationships of New Lipids Designed for DNA Transfection

et al. 2011

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Cationic liposome/DNA complexes can be used as nonviral vectors for direct delivery of DNA-based biopharmaceuticals to damaged cells and tissues. To obtain more effective and safer liposome-based gene transfection systems, two cationic lipids with identical head groups but different chain structures are investigated with respect to their in vitro gene-transfer activity, their cell-damaging characteristics, and their physicochemical properties. The gene-transfer activities of the two lipids are very different. Differential scanning calorimetry and synchrotron small- and wide-angle X-ray scattering give valuable structural insight. A subgel-like structure with high packing density and high phase-transition temperature from gel to liquid-crystalline state are found for lipid 7 (N'-2-[(2,6-diamino-1-oxohexyl)amino]ethyl-2,N-bis(hexadecyl)propanediamide) containing two saturated chains. Additionally, an ordered head-group lattice based on formation of a hydrogen-bond network is present. In contrast, lipid 8 (N'-2-[(2,6-diamino-1-oxohexyl)amino]ethyl-2-hexadecyl-N-[(9Z)-octadec-9-enyl]propanediamide) with one unsaturated and one saturated chain shows a lower phase-transition temperature and a reduced packing density. These properties enhance incorporation of the helper lipid cholesterol needed for gene transfection. Both lipids, either pure or in mixtures with cholesterol, form lamellar phases, which are preserved after addition of DNA. However, the system separates into phases containing DNA and phases without DNA. On increasing the temperature, DNA is released and only a lipid phase without intercalated DNA strands is observed. The conversion temperatures are very different in the two systems studied. The important parameter seems to be the charge density of the lipid membranes, which is a result of different solubility of cholesterol in the two lipid membranes. Therefore, different binding affinities of the DNA to the lipid mixtures are achieved.

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“…44 The removal of the bulk CTAB will cause aggregation of GNRs 3 . Recently, it was found that the thiol-terminated methoxypolyethylene glycol (PEG) can be a stabilizer for GNRs.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional optoacoustic imaging as a new noninvasive technique to study long-term biodistribution of optical contrast agents in small animal models

Ermilov

Liopo

et al. 2012

J. Biomed. Opt.

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Biophysical and biochemical properties of a binary lipid mixture for DNA transfection

Cited by 68 publications

References 57 publications

Synthesis, Assembly, and Biofunctionalization of Silica-Coated Gold Nanorods for Colorimetric Biosensing

Synthesis, Assembly, and Biofunctionalization of Silica-Coated Gold Nanorods for Colorimetric Biosensing

Structure–Function Relationships of New Lipids Designed for DNA Transfection

Three-dimensional optoacoustic imaging as a new noninvasive technique to study long-term biodistribution of optical contrast agents in small animal models

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