Synthesis, Assembly, and Biofunctionalization of Silica-Coated Gold Nanorods for Colorimetric Biosensing

Wang, Chungang; Ma, Zhanfang; Wang, Tingling; Su, Zhong‐Min

doi:10.1002/adfm.200500898

Cited by 188 publications

(146 citation statements)

References 49 publications

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“…For example, gold nanorods have two principal plasmon absorption bands that are observed in the visible region of the electromagnetic spectra, these bands are related to the orientation of the rods. This characteristic was employed to the determination of human immunoglobulin G (IgG), which is used as a protein model by Wang et al 25 The authors have detected IgG by using silica-coated gold nanorods deposited on poly(4-vinylpyridine) (PVP) film on quartz substrates. The subsequent covalent bioconjugation of amino-functionalized gold nanorods-silica films with goat anti-human IgG is employed for the optical detection of antigen once there is a gradual absorbance change as the antigen concentration increases.…”

Section: Opticalmentioning

confidence: 99%

Biosensors based on gold nanostructures

Vidotti¹,

Carvalhal²,

Ferreira³

et al. 2011

J. Braz. Chem. Soc.

130

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O presente trabalho de revisão aborda os mais recentes avanços na tecnologia de biossensores alcançados através da montagem de biomoléculas associada com nanopartículas de ouro na construção de dispositivos analíticos. Esta revisão está dividida de acordo com a biomolécula empregada no desenvolvimento de biossensores: (i) compostos imunológicos; (ii) DNA/RNA funcionais; e (iii) enzimas e proteínas Heme. A fim de facilitar a compreensão, cada seção foi subdividida de acordo com o modo de transdução. Os imunossensores contendo nanopartículas de ouro têm uma ampla gama de aplicações nos campos alimentício, ambiental, farmacêutico, químico e de diagnósticos clínicos. As nanopartículas foram empregadas para melhorar o sinal analítico ou a imobilização dos imunocompostos. Em outra seção, os biossensores DNA/ RNA empregando nanoestruturas de ouro como labels e biossensores label-free associados a nanoestruturas de ouro como transdutores foram sistematicamente relatados para a rápida identificação de patógenos, espécies de interesse ambiental e diagnóstico clínico. A inclusão de nanopartículas de ouro em eletrodos modificados aumenta a transferência de elétrons entre o transdutor e a biomolécula proporcionando um melhor desempenho quando enzimas e proteínas redox heme são usados. Biossensores para a detecção e quantificação de glicose e peróxido de hidrogênio foram também discutidos.The present review discusses the latest advances in biosensor technology achieved by the assembly of biomolecules associated with gold nanoparticles in analytical devices. This review is divided in sections according to the biomolecule employed in the biosensor development: (i) immunocompounds; (ii) DNA/RNA and functional DNA/RNA; and (iii) enzymes and Heme proteins. In order to facilitate the comprehension each section was subdivided according to the transduction mode. Gold nanoparticles based immunosensors have a wide range of applications in food, environmental, pharmaceutical, chemistry and clinical diagnostics. The nanoparticles were employed to improve whether the analytical signal or the immunocompounds immobilization. In another section, biosensors based on DNA/RNA biomolecules employing gold nanostructures as labels and label-free funtional DNA/RNA biosensors associated to gold nanostructures as tranducers were systematically reported for rapid identification of pathogens, species of environmental interest and clinical diagnostics, respectively. The inclusion of gold nanoparticles in modified electrodes itself enhances the electron transfer between the transducer and biomolecules leading to improved bioanalytical devices when redox enzymes and heme proteins are used. Biosensors for the detection and quantification of glucose and hydrogen peroxide are discussed as well.

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Section: Opticalmentioning

confidence: 99%

Biosensors based on gold nanostructures

Vidotti¹,

Carvalhal²,

Ferreira³

et al. 2011

J. Braz. Chem. Soc.

130

View full text Add to dashboard Cite

show abstract

“…30 A tetraethoxysilane/ethanol solution (1 mL) was added to the diluted GNR system, which was adjusted to pH 10 with 0.1 M NaOH. The resulting mixture was gently stirred for 24 hours.…”

Section: Synthesis Of Gnrs@msiomentioning

confidence: 99%