Biomimicry of microbial polysaccharide hydrogels for tissue engineering and regenerative medicine – A review

Ng, Jack Kit-Chung; Obuobi, Sybil; Chua, Mei Ling; Zhang, Chi; Hong, Shiqi; Kumar, Yogesh; Gokhale, Rajeev; Ee, Pui Lai Rachel

doi:10.1016/j.carbpol.2020.116345

Cited by 104 publications

(59 citation statements)

References 191 publications

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“…A cell-adhesive gellan gum-collagen hydrogel scaffold was used for in vitro 3D cell culture. The hydrogel matrix was prepared as previously reported (Ng et al, 2019). Briefly, 60 µL of cellladen hydrogels were formed in each well of 96-well plate (HDF) or 48-well plate (hMSC or ADSC), and supplemented with 200 and 600 µL of culture media respectively.…”

Section: Cell Encapsulation Into Hydrogel Matrix For In Vitro 3d Cellmentioning

confidence: 99%

“…For the artificial construction of living tissues, the tri-component recipe of a tissue scaffold, stem cells, and animal-derived growth factors are quintessential (Ng et al, 2020). To replicate 3D cell culture, MSCs encapsulated in a previously reported hybrid gellan gum-collagen hydrogel (Ng et al, 2019) were grown with and without CGF in both full and reduced serum conditions. The hydrogels exhibited consistent high degree of porosity (Supplementary Figure S4) with a maximal pore diameter of 150 µm and did not impede the effective movement of relatively smaller sized bioactive components of CGF ( Supplementary Table S3) in the matrix to reach the encapsulated mammalian cells according to the Franz cell diffusion assay (Supplementary Figure S5).…”

Section: Cgf Supports 3d Cell Culture For Term Applicationsmentioning

confidence: 99%

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Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

Chua

Zhang³

et al. 2020

Front. Bioeng. Biotechnol.

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The global cell culture market is experiencing significant growth due to the rapid advancement in antibody-based and cell-based therapies. Both rely on the capacity of different living factories, namely prokaryotic and eukaryotic cells, plants or animals for reliable and mass production. The ability to improve production yield is of important concern. Among many strategies pursued, optimizing the complex nutritional requirements for cell growth and protein production has been frequently performed via culture media component titration and serum replacement. The addition of specific ingredients into culture media to modulate host cells' metabolism has also recently been explored. In this study, we examined the use of extracted bioactive components of the microalgae Chlorella vulgaris, termed chlorella growth factor (CGF), as a cell culture additive for serum replacement and protein expression induction. We first established a chemical fingerprint of CGF using ultraviolet-visible spectroscopy and liquid chromatography-mass spectrometry and evaluated its ability to enhance cell proliferation in mammalian host cells. CGF successfully promoted the growth of Chinese hamster ovary (CHO) and mesenchymal stem cells (MSC), in both 2D and 3D cell cultures under reduced serum conditions for up to 21 days. In addition, CGF preserved cell functions as evident by an increase in protein expression in CHO cells and the maintenance of stem cell phenotype in MSC. Taken together, our results suggest that CGF is a viable culture media additive and growth matrix component, with wide ranging applications in biotechnology and tissue engineering.

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Section: Cell Encapsulation Into Hydrogel Matrix For In Vitro 3d Cellmentioning

confidence: 99%

Section: Cgf Supports 3d Cell Culture For Term Applicationsmentioning

confidence: 99%

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

Chua

Zhang³

et al. 2020

Front. Bioeng. Biotechnol.

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“…Microbial polysaccharides are mainly exo-polysaccharides that bacteria secrete for their own purposes henceforth they do not provoke a biological response from cells, so they do not biologically interact with human tissues. To overcome this drawback, scientists have embedded bioactive substances or inorganic materials into the scaffolds based on bacterial polysaccharides via physical and chemical strategies [ 176 ]. Electrospun nanofibers (eNFs) obtained from bacterial biodegradable polymers have attracted a privileged attention for the wound healing field as they are non-antigenic, histo-compatible, and readily washed from the wound area [ 177 ].…”

Section: Electrospun Nanofibers In Wound Healingmentioning

confidence: 99%

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

et al. 2020

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Currently, despite the thoroughgoing scientific research carried out in the area of wound healing management, the treatment of skin injuries, regardless of etiology remains a big provocation for health care professionals. An optimal wound dressing should be nontoxic, non-adherent, non-allergenic, should also maintain a humid medium at the wound interfacing, and be easily removed without trauma. For the development of functional and bioactive dressings, they must meet different conditions such as: The ability to remove excess exudates, to allow gaseous interchange, to behave as a barrier to microbes and to external physical or chemical aggressions, and at the same time to have the capacity of promoting the process of healing by stimulating other intricate processes such as differentiation, cell adhesion, and proliferation. Over the past several years, various types of wound dressings including hydrogels, hydrocolloids, films, foams, sponges, and micro/nanofibers have been formulated, and among them, the electrospun nanofibrous mats received an increased interest from researchers due to the numerous advantages and their intrinsic properties. The drug-embedded nanofibers are the potential candidates for wound dressing application by virtue of: Superior surface area-to volume ratio, enormous porosity (can allow oxy-permeability) or reticular nano-porosity (can inhibit the microorganisms’adhesion), structural similitude to the skin extracellular matrix, and progressive electrospinning methodology, which promotes a prolonged drug release. The reason that we chose to review the formulation of electrospun nanofibers based on polysaccharides as dressings useful in wound healing was based on the ever-growing research in this field, research that highlighted many advantages of the nanofibrillary network, but also a marked versatility in terms of numerous active substances that can be incorporated for rapid and infection-free tissue regeneration. In this review, we have extensively discussed the recent advancements performed on electrospun nanofibers (eNFs) formulation methodology as wound dressings, and we focused as well on the entrapment of different active biomolecules that have been incorporated on polysaccharides-based nanofibers, highlighting those bioagents capable of improving the healing process. In addition, in vivo tests performed to support their increased efficacy were also listed, and the advantages of the polysaccharide nanofiber-based wound dressings compared to the traditional ones were emphasized.

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“…Tissue engineering applications require the use of complex scaffolds where an accurate selection of materials is essential. In addition, it is advantageous that these materials could accommodate growth factors and cells, while providing cues to guide cell adhesion and proliferation [9,10].…”

Section: Biomimetic Hybrid Systems Based On Natural Polymers For Tissmentioning

confidence: 99%

“…Nevertheless, chemical modifications appear necessary to facilitate the attachment of cells, which may be a problematic feature since a complete removal of employed toxic agents is essential for subsequent biomedical applications [14]. Direct blending of polysaccharides is a recent strategy that is applied to improve cellular adhesion and proliferation as recently reviewed by Ng et al [9]. Collagen is the most relevant structural protein in the human body [26] and is the major component of the native ECM.…”

Section: Biomimetic Hybrid Systems Based On Natural Polymers For Tissmentioning

confidence: 99%

Biomimetic Hybrid Systems for Tissue Engineering

2020

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Tissue engineering approaches appear nowadays highly promising for the regeneration of injured/diseased tissues. Biomimetic scaffolds are continuously been developed to act as structural support for cell growth and proliferation as well as for the delivery of cells able to be differentiated, and also of bioactive molecules like growth factors and even signaling cues. The current research concerns materials employed to develop biological scaffolds with improved features as well as complex preparation techniques. In this work, hybrid systems based on natural polymers are discussed and the efforts focused to provide new polymers able to mimic proteins and DNA are extensively explained. Progress on the scaffold fabrication technique is mentioned, those processes based on solution and melt electrospinning or even on their combination being mainly discussed. Selection of the appropriate hybrid technology becomes vital to get optimal architecture to reasonably accomplish the final applications. Representative examples of the recent possibilities on tissue regeneration are finally given.

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Biomimicry of microbial polysaccharide hydrogels for tissue engineering and regenerative medicine – A review

Cited by 104 publications

References 191 publications

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

Chlorella vulgaris Extract as a Serum Replacement That Enhances Mammalian Cell Growth and Protein Expression

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

Biomimetic Hybrid Systems for Tissue Engineering

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