Biomimetically synthesized ZnO nanoparticles attain potent antibacterial activity against less susceptible S. aureus skin infection in experimental animals

Rauf, Mohd Ahmar; Owais, Mohammad; Rajpoot, Ravikant; Faraz, Ahmad; Khan, Nabiullah; Zubair, Swaleha

doi:10.1039/c7ra05040b

Cited by 103 publications

(43 citation statements)

References 51 publications

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“…The synthesized ZnONPs also inhibited the biolm synthesis of tested isolates in vitro and successfully treated the S. aureusmediated experimental skin infection in BALB/c mice. 31 The penetration of CEN-ZnO NPs into the bacterial cells (in line with the results obtained from ow cytometry analysis), increases intracellular accumulation of nanoparticles, thereby destroying bacterial cells. 32 Malaikozhundan et al 33 tested the cytotoxic effects of Pp-ZnO NPs on MCF-7 breast cancer cells and found that single-treatment with Pp-ZnO at concentrations greater than 50 mg ml À1 reduces cell viability.…”

Section: Discussionsupporting

confidence: 66%

A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

et al. 2019

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Cyanobacteria, as one of the largest groups of phototrophic bacteria, have a high potential as an excellent source of fine chemicals and bioactive compounds, including lipid-like compounds, amino acid derivatives, proteins, and pigments. This study aimed to synthesize ZnO nanoparticles using the cell extract of the cyanobacterium Nostoc sp. EA03 (CEN-ZnO NPs) through a rapid and eco-friendly approach. The biosynthesized nanoparticles, CEN-ZnO NPs, were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), zeta potential measurement, differential scanning calorimetry (DSC)/thermogravimetric analysis (TGA), FTIR, SEM, TEM, and EDX spectroscopy. The UV-Vis spectrum showed an absorption peak at 370 nm. The star-shaped CEN-ZnO NPs, as observed in the TEM and SEM images, had an average diameter of 50-80 nm. MIC and MBC values for E. coli, P. aeruginosa and S. aureus, were determined to be, respectively, 2000, 2000, and 64 mg ml À1 , and 2500, 2500 and 128 mg ml À1 . Further analysis through confocal laser scanning microscopy (CLSM) provided the observable confirmation that the CEN-ZnO NPs stunted the bacterial growth, preventing the formation of exopolysaccharides. The AFM analysis of surface topography of bacterial biofilm samples treated with CEN-ZnO NPs showed a rugged topography in some parts of the biofilm surface, indicating the destruction of biofilms. In contrast, in the untreated control samples, the structured biofilms were flat and prominent. MTT assay indicated that CEN-ZnO NPs had less cytotoxicity on the MRC-5 lung fibroblast cells compared with the cancerous treated A549 cells. As the concentration of the CEN-ZnO NPs increased, the amount of ROS produced in the tested bacterial strains also increased. Analyzing the data obtained from flow cytometry showed that the higher concentrations of CEN-ZnO NPs lead to a reduction in the viability of P. aeruginosa PAO1, E. coli and S. aureus. The biosynthesized ZnO nanoparticles using Nostoc cell extracts exhibited different attributes, inspiring enough to be considered for further investigation.

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Section: Discussionsupporting

confidence: 66%

A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

et al. 2019

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“…Finally, bandgap of prepared nanomaterials were calculated by Tauc transformation spectra and extrapolation used on X-axis. Energy bandgap of pure ZnO found 3.28 eV [43] and with GO bandgap energy increased around 3.32 eV.…”

Section: Resultsmentioning

confidence: 91%

Enhanced drug efficiency of doped ZnO–GO (graphene oxide) nanocomposites, a new gateway in drug delivery systems (DDSs)

Afzal

Ikram

Ali

et al. 2020

Mater. Res. Express

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Graphene oxide (GO) nanosheets were doped to zinc oxide (ZnO) nanoparticles using a facile chemical deposition method. X-ray diffraction (XRD) confirmed the presence of hexagonal structure and increased peaks broadening upon doping. Undoped and ZnO doped GO sheets showed morphology like nanoparticles, nanorods and flakes were observed under transmission and field emission electron microscopies respectively. An increase in absorption was observed in absorption spectra upon doping recorded via UV-visible spectroscopy. The hydrogen bonding between functional groups of GO and ZnO is responsible for limiting maximum drug loading efficiency. GO doped ZnO has higher drug loading efficiency of about 89% compared to ZnO (82%) and this trend reverse in drug releasing process. This study will provide an efficient design of the drug delivery system for dissolution enhancement according to the required drug release.

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“…Sterile disks were impregnated with ZnO-NRs and DAS emulsion and placed on bacteria harboring plates followed by incubation at 37°C for 24 h. Antibacterial activity was expressed in terms of diameter of the zone of inhibition, measured in millimeters. The assays were performed in triplicate ( Wiegand et al, 2008 ; Rauf et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…After the stipulated incubation time, the cells were washed with cold sterile PBS and mounted on glass slide. The cells were observed under fluorescence microscope (Zeiss, United States) ( Miller and Quarles, 1990 ; Rauf et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…For histopathological studies, infected skin tissues was sliced (from mice belonging to various groups) and fixed in 10% formaldehyde solution, dehydrated in ascending grades of ethyl alcohol, cleared in xylol and mounted in molten paraplast at 58–62°C. The finely cut thin section was stained with hematoxylin and eosin stain and evaluated for any morphological changes under an Olympus BX40 microscope (PA, United States) for the comparative study ( Kugelberg et al, 2005 ; Rauf et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

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Synergistic Effect of Diallyl Sulfide With Zinc Oxide Nanorods: A Novel and Effective Approach for Treatment of Acute Dermatitis in Model Animals

et al. 2018

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Besides inciting persistent and recurrent nosocomial afflictions, Staphylococcus aureus (S. aureus), a biofilm forming pathogen, poses an increased risk of several skin as well as respiratory tract infections as well. Emerging antimicrobial resistance trend asks to search for an alternate non-antibiotic based option to combat S. aureus pathogen. In the present study, we evaluated synergistic antimicrobial potential of Zinc oxide nanorods (ZnO-NRs) and diallyl sulphide (DAS) emulsion against methicillin resistant Staphylococcus aureus (MRSA). The antimicrobial assessment study suggests that the ZnO-NR and DAS emulsion effectively suppressed both sensitive S. aureus as well as MRSA isolates. The combination treatment showed enhanced activity even at a lower concentration as compared to the single treatment based on ZnO-NRs and DAS emulsion alone. The ZnO-NRs-DAS combination showed significant inhibition of MRSA biofilm as well. The data suggest that a combination therapy, comprising of ZnO-NRs and DAS emulsion, successfully treated experimental dermatitis infection caused by MRSA in mice model.

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Biomimetically synthesized ZnO nanoparticles attain potent antibacterial activity against less susceptible S. aureus skin infection in experimental animals

Abstract: Biomimetic synthesis of ZnO–NPs and their topical application on S. aureus induced skin infection leads to reduction in infection in mice model.

Cited by 103 publications

References 51 publications

A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation

Enhanced drug efficiency of doped ZnO–GO (graphene oxide) nanocomposites, a new gateway in drug delivery systems (DDSs)

Synergistic Effect of Diallyl Sulfide With Zinc Oxide Nanorods: A Novel and Effective Approach for Treatment of Acute Dermatitis in Model Animals

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