Biomimetic poly(glycerol sebacate) (PGS) membranes for cardiac patch application

Abstract

In this study biomimetic poly(glycerol sebacate) PGS matrix was developed for cardiac patch application. The rationale was that such matrices would provide conducive environment for the seeded cells at the interphase with PGS. From the microstructural standpoint, PGS was fabricated into dense films and porous PGS scaffolds. From the biological aspect, biomimetic PGS membranes were developed via covalently binding peptides Tyr-Ile-Gly-Ser-Arg (YIGSR) and Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP), corresponding to the epitope sequences of laminin and fibronectin, respectively onto the surface. To improve and enhance homogenous binding of peptides onto the PGS surface, chemical modification of its surface was carried out. A sequential regime of alkaline hydrolysis with 0.01 M NaOH for 5 min and acidification with 0.01 M HCl for 25s was optimal. More COOH chemical group was exposed without causing deleterious effect on the bulk properties of the polymer as revealed by the physicochemical analysis carried out. HPLC analysis, chemical imaging and ToF-SIMS were able to establish the successful homogenous functionalization of PGS membranes with the peptides. Finally, the developed biomimetic membranes supported the adhesion and growth of rat and human cardiac progenitor cells.

Poly(glycerol sebacate) biomaterial: synthesis and biomedical applications

“…These scaffolds were combined with heart cells, and cultured with perfusion to engineer contractile cardiac muscle constructs. 61 The adhesion and growth of rat and human cardiac progenitor cells were well supported on the biomimetic membranes than unmodified PGS scaffolds. The second layer consisted of fully interconnected 3D pore meshes fabricated by oxygen plasma treatment of the first layer of scaffolds followed by stacking with off-set laminae to produce a tightly bonded composite.…”

Additive manufactured biodegradable poly(glycerol sebacate methacrylate) nerve guidance conduits

“…Sebacic acid copolymers have been FDA approved for use as drug delivery matrices [19], The use of PGS in tissue engineering has also been explored for cardiac patches [20,21], microfluidics [22], tympanic perforations [23], drug delivery [19], retinal transplantation [24] and neural repair [25]. PGS has previously been explored for its use as nerve guide material by Sundback et al [25].…”

Vibrational spectroscopy and imaging: applications for tissue engineering

“…In the tissue engineering field, MIR spectroscopy has been used for characterizing biomaterials and scaffolds, and ECM. This includes constructs designed for articular cartilage 48–50 , bone 51, 52 , heart 53, 54 , skin 55, 56 , cornea 57, 58 , and bladder 59 . However, the use of MIR to evaluate ECM produced by cells on engineered tissue constructs has focused primarily on studies of articular cartilage and bone, which is what we focus on here.…”