Additive manufactured biodegradable poly(glycerol sebacate methacrylate) nerve guidance conduits

Singh, Dharaminder; Harding, Adam J.; Albadawi, Emad; Boissonade, Fiona M.; Haycock, John W.; Claeyssens, Frederik

doi:10.1016/j.actbio.2018.07.055

Cited by 91 publications

(96 citation statements)

References 50 publications

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“…[7][8][9]32,33,37,44 Additionally, it would be important to evaluate a combination of strategies to facilitate the application of oral-derived NCSCs in regenerative medicine, such as their incorporation in advanced manufactured scaffolds or conduits for nerve repair and tissue engineering (eg, bone regeneration). 9,45,46 Finally, the field of dental pulp stem cells and their applications would benefit from addressing specific diseases or targeted applications from preclinically relevant established cultures if we aim to advance the use of dental pulp cells in regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Solis-Castro

Boissonade

Rivolta

2020

Stem Cells Translational Medicine

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The potential of obtaining cell cultures with neural crest resemblance (neural crestderived stem cells [NCSCs]) from dental-related tissues, including human dental pulp cells (hDPCs), has been discussed in the literature. However, most reports include the use of serum-rich conditions and do not describe the potential for neural differentiation, slowing translation to the clinic. Therefore, we aimed to culture and characterize NCSCs from the human dental pulp in vitro and evaluate their abil

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Section: Discussionmentioning

confidence: 99%

Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Solis-Castro

Boissonade

Rivolta

2020

Stem Cells Translational Medicine

Self Cite

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“…Many grafts have been developed to facilitate nerve regeneration and sustain a pro-regenerative environment in the distal segment. 33,[41][42][43][44][45][46] However, there has been limited focus on the ability for a graft to influence the intrinsic regenerative capacity of the proximal neuron. To the best of our knowledge, this is the first study to investigate the effect of different peripheral nerve repair strategies on the proximal neuron health and regenerative capacity.…”

Section: Discussionmentioning

confidence: 99%

Tissue Engineered Axon-Based “Living Scaffolds” Promote Survival of Spinal Cord Motor Neurons Following Peripheral Nerve Repair

Maggiore

Burrell

Browne

et al. 2019

Preprint

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Peripheral nerve injury (PNI) impacts millions annually, often leaving debilitated patients with minimal repair options to improve functional recovery. Our group has previously developed tissue engineered nerve grafts (TENGs) featuring long, aligned axonal tracts from dorsal root ganglia (DRG) neurons that are fabricated in custom bioreactors using the process of axon "stretch-growth". We have shown that TENGs effectively serve as "living scaffolds" to promote regeneration across segmental nerve defects by exploiting the newfound mechanism of axonfacilitated axon regeneration, or "AFAR", by simultaneously providing haptic and neurotrophic support. To extend this work, the current study investigated the efficacy of living versus non-living regenerative scaffolds in preserving host sensory and motor neuron cell health, using retrograde dye transport as a proxy, following bridging of segmental nerve defects. Rats were assigned across five groups: naïve or repair using autograft, nerve guidance tube (NGT) with collagen, NGT + non-aligned DRG populations in collagen, or TENGs. We found that TENG repairs yielded equivalent regenerative capacity as autograft repairs based on preserved health of host spinal cord motor neurons and acute axonal regeneration, whereas NGT repairs or DRG neurons within an NGT exhibited reduced motor neuron preservation and diminished regenerative capacity.These acute regenerative benefits ultimately resulted in enhanced levels of functional recovery in animals receiving TENGs, at levels matching those attained by autografts. Our findings indicate that when used to bridge segmental nerve defects, TENGs preserve host spinal cord motor neuron health and regenerative capacity without sacrificing an otherwise uninjured nerve (as in the case of the autograft), and therefore represent a promising alternative strategy for neurosurgical repair following PNI.2 HIGHLIGHTS

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“…Poly(glycerol sebacate) (PGS) elastomer has been intensively studied in biomedical applications such as nerve conduits, heart patches, drug delivery systems, and adhesives, among others. [ 1–7 ] Of particular interest in our lab is to engineer an acellular fast‐degrading PGS vascular graft for small‐diameter arteries. [ 8–14 ] The synthetic graft is fully remodeled by host cells in months post‐implantation.…”

Section: Introductionmentioning

confidence: 99%

Control the Mechanical Properties and Degradation of Poly(Glycerol Sebacate) by Substitution of the Hydroxyl Groups with Palmitates

Ding

Chen

Chao

et al. 2020

Macromolecular Bioscience

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Mechanical properties and degradation profile are important parameters for the applications of biodegradable polyester such as poly(glycerol sebacate) in biomedical engineering. Here, a strategy is reported to make palmitate functionalized poly(glycerol sebacate) (PPGS) to alter the polymer hydrophobicity, crystallinity, microstructures and thermal properties. The changes of these intrinsic properties impart tunable degradation profiles and mechanical properties to the resultant elastomers depending on the palmitate contents. When the palmitates reach up to 16 mol%, the elastic modulus is tuned from initially 838 ± 55 kPa for the PGS to 333 ± 21 kPa for the PPGS under the same crosslinking conditions. The elastomer undergoes reversible elastic deformations for at least 1000 cycles within 20% strain without failure and shows enhanced elasticity. The polymer degradation is simultaneously inhibited because of the increased hydrophobicity. This strategy is different with other PGS modifications which could form a softer elastomer with less crosslinks but typically lead to a quicker degradation. Because the materials are made from endogenous molecules, they possess good cytocompatibility similar to the PGS control. Although these materials are designed specifically for small arteries, it is expected that they will be useful for other soft tissues too.

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Additive manufactured biodegradable poly(glycerol sebacate methacrylate) nerve guidance conduits

Cited by 91 publications

References 50 publications

Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Tissue Engineered Axon-Based “Living Scaffolds” Promote Survival of Spinal Cord Motor Neurons Following Peripheral Nerve Repair

Control the Mechanical Properties and Degradation of Poly(Glycerol Sebacate) by Substitution of the Hydroxyl Groups with Palmitates

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