Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Solis-Castro, Oscar O.; Boissonade, Fiona M.; Rivolta, Marcelo N.

doi:10.1002/sctm.20-0037

Cited by 21 publications

(21 citation statements)

References 46 publications

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“…Additionally, it was found that a neural-like differentiation was more evident in cultures from serum-free conditions supplemented with BMP4 as judged by a more defined morphological change and the upregulation of GAP43 , NFH and SYN1 mRNA. The mentioned report presented a broader characterization of the NCSC phenotype in human dental pulp cells, and also supported how 3D culturing is important to enhance a NCSC signature and downstream differentiation to neural-like cells [ 40 ].…”

Section: Dental-related Sources Of Human Neural Crest-derived Stem Cellsmentioning

confidence: 76%

“…A publication from our group explored in more detail the NCSC phenotype in human dental pulp cells as monolayer and as neurosphere cultures [ 40 ]. Contrary to most reports, the tissue was established from the beginning into neurogenic, serum-free culture conditions.…”

Section: Dental-related Sources Of Human Neural Crest-derived Stem Cellsmentioning

confidence: 99%

“…Results from human gingival and apical pulp-derived NCSCs suggests that neurosphere formation increased NCSC markers and decreased mesenchymal markers, indicating a molecular resemblance to a neural crest phenotype [ 25 , 32 ]. Notably, some authors describe an intrinsic ability of the monolayer cells to detach from the surface ( Figure 3 A; [ 26 ]) while others grow the cells directly in suspension in low-attachment culture wells ( Figure 3 B; [ 37 , 40 ]).…”

Section: Considerations Of Growth Conditions and Strategies For Isola...mentioning

confidence: 99%

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Neural Crest-Derived Stem Cells (NCSCs) Obtained from Dental-Related Stem Cells (DRSCs): A Literature Review on Current Knowledge and Directions toward Translational Applications

Solis-Castro

Rivolta

Boissonade

2022

IJMS

Self Cite

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Evidence from dental-related stem cells (DRSCs) suggests an enhanced potential for ectodermal lineage differentiation due to their neural crest origin. Growing evidence that DRSC cultures can produce cells with a neural crest-derived stem cell (NCSC)-like phenotype supports their potential for future therapeutic approaches for neurodegenerative diseases and nerve injuries. However, most of the evidence is limited to the characterization of DRSCs as NCSCs by detecting the expression of neural crest markers. Only a few studies have provided proof of concept of an improved neuro-glial differentiation or direct applicability in relevant models. In addition, a current problem is that several of the existing protocols do not meet manufacturing standards for transferability to a clinical scenario. This review describes the current protocols to obtain NCSCs from DRSCs and their characterization. Also, it provides important considerations from previous work where DRSCs were established and characterized as mesenchymal stromal cells but studied for their neuro-glial differentiation potential. The therapeutic advancement of DRSCs would depend on establishing protocols that can yield a neural crest-like phenotype efficiently, using appropriate manufacturing standards and testing them in relevant models of disease or injury. Achieving these conditions could then facilitate and validate the therapeutic potential of DRSC-NCSCs in regenerative therapies.

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Section: Dental-related Sources Of Human Neural Crest-derived Stem Cellsmentioning

confidence: 76%

Section: Dental-related Sources Of Human Neural Crest-derived Stem Cellsmentioning

confidence: 99%

Section: Considerations Of Growth Conditions and Strategies For Isola...mentioning

confidence: 99%

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Neural Crest-Derived Stem Cells (NCSCs) Obtained from Dental-Related Stem Cells (DRSCs): A Literature Review on Current Knowledge and Directions toward Translational Applications

Solis-Castro

Rivolta

Boissonade

2022

IJMS

Self Cite

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“…Developmentally, most cranial/dental tissues are derived from neural crest (37,38). A unique subpopulation of multipotent mesenchymal stem cells with NC-derived stem cell properties have been isolated from various adult human dental tissues, including dental pulp (DPSCs) (12,40), oral mucosa and gingiva (41)(42)(43)(44), apical papilla (APSC) (45), dental follicle (DFSC) (46), and periodontal ligament (PDLSC) (47). These studies suggest that dental tissues serve as important reservoirs of NC-derived multipotent stem cells.…”

Section: Isolation and Characterization Of Human Gingiva-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

Kim

Lee

et al. 2021

Front. Immunol.

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A unique subpopulation of mesenchymal stem cells (MSCs) has been isolated and characterized from human gingival tissues (GMSCs). Similar to MSCs derived from other sources of tissues, e.g. bone marrow, adipose or umbilical cord, GMSCs also possess multipotent differentiation capacities and potent immunomodulatory effects on both innate and adaptive immune cells through the secretion of various types of bioactive factors with immunosuppressive and anti-inflammatory functions. Uniquely, GMSCs are highly proliferative and have the propensity to differentiate into neural cell lineages due to the neural crest-origin. These properties have endowed GMSCs with potent regenerative and therapeutic potentials in various preclinical models of human disorders, particularly, some inflammatory and autoimmune diseases, skin diseases, oral and maxillofacial disorders, and peripheral nerve injuries. All types of cells release extracellular vesicles (EVs), including exosomes, that play critical roles in cell-cell communication through their cargos containing a variety of bioactive molecules, such as proteins, nucleic acids, and lipids. Like EVs released by other sources of MSCs, GMSC-derived EVs have been shown to possess similar biological functions and therapeutic effects on several preclinical diseases models as GMSCs, thus representing a promising cell-free platform for regenerative therapy. Taken together, due to the easily accessibility and less morbidity of harvesting gingival tissues as well as the potent immunomodulatory and anti-inflammatory functions, GMSCs represent a unique source of MSCs of a neural crest-origin for potential application in tissue engineering and regenerative therapy.

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“…Neural crest stem cells (NCSCs) are known as a kind of multipotent progenitor cells that give rise various phenotypic lineages including SCs. In the craniofacial region, these stem cells are found in some tissues such as dental pulp (11)(12)(13), periodontal ligament (11), dermal papilla (14) and nasal mucosa (15). Olfactory ecto-mesenchymal stem cells (OE-MSCs) are a new population of neural crest-derived stem cells (15) that can be obtained from the olfactory nasal mucosa niche using non-invasive tecniques (16,17).…”

Section: Introductionmentioning

confidence: 99%

Human Olfactory Ecto-mesenchymal Stem Cells Displaying Schwann-cell-like Phenotypes and Promoting Neurite Outgrowth in Vitro

Entezari¹,

Cellular²,

Bakhtiari³

et al. 2023

BCN

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Strategies of Schwann cell (SC) transplantation for regeneration of peripheral nerve injury involve many limitations. Stem cells can be used as alternative cell source for diﬀerentiation into Schwann cells. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ectomesenchymal stem cells (OE-MSCs) derived from neural crest can spontaneously differentiate into SC lineage. OE-MSCs were isolated from human nasal mucosa and characterized by the mesenchymal and neural crest markers. The cells were cultured in glial growth factors-free medium and further investigated in terms of the phenotypic and functional properties. Immunocytochemical staining and real-time PCR analysis indicated that the cultured OE-MSCs expressed SCs markers, SOX10, p75, S100, GFAP and MBP, differentiation indicative. It was found that the cells could secrete neurotrophic factors, including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Furthermore, after co-cultured with PC12, the mean neurite length was enhanced by OE-MSCs. The findings indicated that OE-MSCs could be differentiated spontaneously into SC-like phenotypes, suggesting their applications for transplantation in peripheral nerve injuries.Strategies of Schwann cell (SC) transplantation for regeneration of peripheral nerve injury involve many limitations. Stem cells can be used as alternative cell source for diﬀerentiation into Schwann cells. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ectomesenchymal stem cells (OE-MSCs) derived from neural crest can spontaneously differentiate into SC lineage. OE-MSCs were isolated from human nasal mucosa and characterized by the mesenchymal and neural crest markers. The cells were cultured in glial growth factors-free medium and further investigated in terms of the phenotypic and functional properties. Immunocytochemical staining and real-time PCR analysis indicated that the cultured OE-MSCs expressed SCs markers, SOX10, p75, S100, GFAP and MBP, differentiation indicative. It was found that the cells could secrete neurotrophic factors, including brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Furthermore, after co-cultured with PC12, the mean neurite length was enhanced by OE-MSCs. The findings indicated that OE-MSCs could be differentiated spontaneously into SC-like phenotypes, suggesting their applications for transplantation in peripheral nerve injuries.

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Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions

Cited by 21 publications

References 46 publications

Neural Crest-Derived Stem Cells (NCSCs) Obtained from Dental-Related Stem Cells (DRSCs): A Literature Review on Current Knowledge and Directions toward Translational Applications

Neural Crest-Derived Stem Cells (NCSCs) Obtained from Dental-Related Stem Cells (DRSCs): A Literature Review on Current Knowledge and Directions toward Translational Applications

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

Human Olfactory Ecto-mesenchymal Stem Cells Displaying Schwann-cell-like Phenotypes and Promoting Neurite Outgrowth in Vitro

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