Biomimetic, highly elastic conductive and hemostatic gelatin/rGO-based nanocomposite cryogel to improve 3D myogenic differentiation and guide in vivo skeletal muscle regeneration

Zhao, Xin; Zhang, Zhiyi; Luo, Jinlong; Wu, Zihao; Yang, Zhuofan; Zhou, Shaowen; Tu, Youpeng; Huang, Ying; Han, Yong; Guo, Baolin

doi:10.1016/j.apmt.2022.101365

Cited by 17 publications

(13 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biocompatibility. The biocompatibility of QCS/OD/TOB/ PPY@PDA hydrogels was evaluated by hemocompatibility test and cytocompatibility test, 71 which are detailed in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Growth-Induced Tobramycin Smart Release Self-Healing Hydrogel for Pseudomonas aeruginosa-Infected Burn Wound Healing

et al. 2022

Self Cite

View full text Add to dashboard Cite

Burns are a common health problem worldwide and are highly susceptible to bacterial infections that are difficult to handle with ordinary wound dressings. Therefore, burn wound repair is extremely challenging in clinical practice. Herein, a series of self-healing hydrogels (QCS/OD/TOB/PPY@PDA) with good electrical conductivity and antioxidant activity were prepared on the basis of quaternized chitosan (QCS), oxidized dextran (OD), tobramycin (TOB), and polydopaminecoated polypyrrole nanowires (PPY@PDA NWs). These Schiff base crosslinks between the aminoglycoside antibiotic TOB and OD enable TOB to be slowly released and responsive to pH. Interestingly, the acidic substances during the bacteria growth process can induce the on-demand release of TOB, avoiding the abuse of antibiotics. The antibacterial results showed that the QCS/OD/TOB/PPY@PDA9 hydrogel could kill high concentrations of Pseudomonas aeruginosa (PA), Staphylococcus aureus, and Escherichia coli in a short time and showed a bactericidal effect for up to 11 days in an agar plate diffusion experiment, while showing good in vivo antibacterial activity. Excellent and long-lasting antibacterial properties make it suitable for severely infected wounds. Furthermore, the incorporation of PPY@PDA endowed the hydrogel with near-infrared (NIR) irradiation assisted bactericidal activity of drug-resistant bacteria, conductivity, and antioxidant activity. Most importantly, in the PA-infected burn wound model, the QCS/OD/TOB/PPY@PDA9 hydrogel more effectively controlled wound inflammation levels and promoted collagen deposition, vascular generation, and earlier wound closure compared to Tegaderm dressings. Therefore, the TOB smart release hydrogels with on-demand delivery are extremely advantageous for bacterial-infected burn wound healing.

show abstract

Section: Methodsmentioning

confidence: 99%

Bacterial Growth-Induced Tobramycin Smart Release Self-Healing Hydrogel for Pseudomonas aeruginosa-Infected Burn Wound Healing

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…118 The good cytocompatibility of NNPHs means that they can maintain cell communication, viability, proliferation, and differentiation. 119,120 Blood plays an important role in the transport of nutrients, oxygen, and waste, so it is necessary to maintain the stability of the blood environment. 121,122 The good blood compatibility of NNPHs requires that NNPHs will not cause damage to the blood, such as changing the composition of blood and destabilization of the blood environment, leading to blood coagulation.…”

Section: Biocompatibilitymentioning

confidence: 99%

“…189 To overcome these challenges, researchers fabricated GO and rGO to ameliorate dispersity of graphene related nanomaterials in water and solvents. 82,119 For instance, Liang et al…”

Section: Carbon-based Nanomaterials Of Nnphs For Wound Healingmentioning

confidence: 99%

“…189 To overcome these challenges, researchers fabricated GO and rGO to ameliorate the dispersity of graphene related nanomaterials in water and solvents. 82,119 For instance, Liang et al prepared an injectable NNPH through free radical polymerization using well-dispersed GO, methacrylate gelatin and methacrylate quaternized chitosan to promote the MRSA-infected mouse fullthickness wound healing. The NNPH had electrical conductivity from 0.0097 to 0.1007 S m −1 , inherent antibacterial properties against normal bacteria with a 95% killing ratio and drug resistant bacteria with a >90% killing ratio, and photothermal antibacterial properties against all types of bacteria with a 100% killing ratio aer exposure to NIR light for 10 minutes.…”

Section: Carbon-based Nnphs For Wound Healingmentioning

confidence: 99%

See 1 more Smart Citation

Engineering functional natural polymer-based nanocomposite hydrogels for wound healing

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In that case, temperature cooling helps gelatin to form porous structures and thus better mimic tissue and promote nerve regeneration [12]. Furthermore, this technique has become widespread, as it has also proven to be advantageous for cell protection as well as for the release of biologically active agents [17,36,54,55]. The freeze-drying technique enabled gelatin to form a porous hydrogel, which helped to attract cells responsible for osteogenesis [18,30].…”

Section: Freeze-drying Techniquementioning

confidence: 99%

Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

View full text Add to dashboard Cite

Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

show abstract

Biomimetic, highly elastic conductive and hemostatic gelatin/rGO-based nanocomposite cryogel to improve 3D myogenic differentiation and guide in vivo skeletal muscle regeneration

Cited by 17 publications

References 57 publications

Bacterial Growth-Induced Tobramycin Smart Release Self-Healing Hydrogel for Pseudomonas aeruginosa-Infected Burn Wound Healing

Bacterial Growth-Induced Tobramycin Smart Release Self-Healing Hydrogel for Pseudomonas aeruginosa-Infected Burn Wound Healing

Engineering functional natural polymer-based nanocomposite hydrogels for wound healing

Progress in Gelatin as Biomaterial for Tissue Engineering

Contact Info

Product

Resources

About