Biomass production of Cymbopogon citratus (D.C.) stapf., a medicinal plant, in temporary immersion systems

Quiala, Elisa; Barbón, Raúl; Jiménez, Elio; Feria, Manuel de; Chávez, Maité; Capote, Alina; Pérez, Naivy

doi:10.1079/ivp2006765

Cited by 41 publications

(14 citation statements)

References 7 publications

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“…In the last decade an important number of work has confirmed that temporary immersion stimulates shoot proliferation and growth (see review Etienne and Berthouly, 2002;Quiala et al 2006;Pérez-Alonso et al 2009;Sankar-Thomas and Lieberei, 2011;Yan et al 2011). The high number of shoots obtained in the TIS may be a consequence of the efficient gaseous exchange between the plant tissue and the gas phase inside the vessel.…”

Section: Discussionmentioning

confidence: 98%

“…Different procedures have been developed to avoid these problems (Ziv, 2005). Among them, the use of temporary immersion systems (TIS) to improve in vitro growth and plant quality in different species (see review Etienne and Berthouly, 2002;Quiala et al 2006;Roels et al 2006;Aragón et al 2009, Pérez-Alonso et al, 2009Yan et al, 2010, Sankar-Thomas andLieberei, 2011). However, few forestry species, Eucalyptus sp (Castro and González, 2002;McAlister et al 2005), Pinus radiata D. Don (AitkenChristie and Jones, 1987) and Crescentia cujete L. (Murch et al 2004) have been successfully propagated by TIS.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Quiala

Cañal

Meijón

et al. 2011

Plant Cell Tiss Organ Cult

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In vitro shoot proliferation of Tectona grandis L. (teak) using temporary immersion system (TIS) and different concentrations of BA is presented. Shoots grown on semi-solid MS medium supplemented with 2% (w/v) sucrose and 4.44 µM of BA were used as starting materials. In the TIS, three BA concentrations (2.22, 4.44 and 6.66 µM) and a cytokinin-free medium (CK-free medium) were assayed. A high average number of shoot was reached, applying 4.44 and 6.66 µM of BA (7.7 and 10.3 shoots/explant), respectively. The high BA concentrations decreased the accumulation of phenolic compounds and the deposition of lignin in the vascular cells of the teak shoots. The morphometric analysis by scanning microscopy revealed that the leaves of the shoots cultured in the TIS in Ck-free medium and with 2.22 and 4.44 µM of BA showed elliptical stomata; however, in the leaves developed with 6.66 µM of BA, the stomata were majorly ring-shaped, raised and wide open. Deformed stomata with broken epidermis of the guard cells, typical of hyperhydric leaves, were also observed. The survival percentages after ex vitro rooting on the IBA (492.1 µM) solution were higher in plantlets from CK-free medium and with 2.22 µM of BA, (96.7 and 91.7%, respectively) than those cultured on semi-solid medium (73%). The shoots from both TIS treatments developed a good root system. Plantlets from 6.66 µM of BA showed the lowest survival percentages (60%). A survival percentage was 100% two weeks after transplanting, and three months after ex vitro transfer, the plants were ready for field plantation. Here we present the first report of the successful propagation of teak by TIS.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Quiala

Cañal

Meijón

et al. 2011

Plant Cell Tiss Organ Cult

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“…Bioreactors are usually designed for intensive culture and affording maximal opportunity for monitoring and control over microenvironmental conditions (Paek et al 2005). Several configurations of organ bioreactors are used for this purpose such as temporary immersion systems (Wilken et al 2005;Quiala et al 2006;Cheel et al 2007;Georgiev et al 2008). …”

Section: Introductionmentioning

confidence: 99%

Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems

Pérez-Alonso

Wilken²,

Gerth³

et al. 2009

Plant Cell Tiss Organ Cult

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Cardiotonic glycosides are extracted mostly from leaves of Digitalis plants. Commercial production of bioactive secondary metabolites by traditional agriculture is an inefficient process and can be affected by climatic and soil conditions. Strategies, based on in vitro culture methods, have been extensively studied to improve the production of specific plant derived chemicals. The aim of the present research was to obtain biomass of D. purpurea using the temporary immersion system (TIS) and to determine the content of cardiotonic glycosides (digitoxin, digoxin and lanatoside C) as secondary metabolites of commercial value for the pharmaceutical industry. Shoots were cultured in 1,000 ml TIS during 28 days. The effect of four immersion frequencies (once every 2, 4, 6, and 12 h) was studied. Biomass accumulation was influenced by immersion frequency. The maximum biomass accumulation (values in respect of fresh and dry weight) was obtained with immersions every 4 h (six immersions per day). HPLC analysis revealed the presence of digoxin and digitoxin for all immersion frequencies. No lanatoside C was detected in the biomass cultured in TIS. Digoxin concentrations varied depending on the frequencies tested. In contrast, the digitoxin content showed no dependency on the immersion frequency. Net production of digoxin and digitoxin per TIS were found to be higher with immersions every 4 h. The best net production of digitoxin and digoxin per TIS were 167.6 and 119.9 lg, respectively. The development of organ culture based on temporary immersion system can be a reliable method for the steady production of biomass for cardiotonic glycosides production, which is reported for the first time for Digitalis genus in this communication.

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“…However, in the classes with longest shoots (5-6 cm, 6-7 cm, and higher than 7 cm), a higher regeneration was observed in TIS-TF. Similar results indicating the superiority of TIS for the production of shoots and plantlets with larger size were observed in peach palm (Steinmacher et al 2011), eucalyptus (McAlister et al 2005, Oliveira et al 2011), strawberry (Hanhineva et al 2005), Spanish red cedar (Peña-Ramíres et al 2010), lemon grass (Quiala et al 2006), sugarcane (Lorenzo et al 1998) and rubber tree (Etienne et al 1997). It was also observed that TIS-TF resulted in greater stability in the regeneration rate between different classes of shoot height.…”

Section: In Vitro Phase: Shoot Regenerationmentioning

confidence: 54%

Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

Scherer

Holderbaum

Garcia

et al. 2015

Crop Breed. Appl. Biotechnol.

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Biomass production of Cymbopogon citratus (D.C.) stapf., a medicinal plant, in temporary immersion systems

Cited by 41 publications

References 7 publications

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems

Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

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