Biomarkers in Nile Tilapia &amp;lt;i&amp;gt;Oreochromis niloticus niloticus&amp;lt;/i&amp;gt; (Linnaeus, 1758) to Assess the Impacts of River Nile Pollution: Bioaccumulation, Biochemical and Tissues Biomarkers

Shehata

Egypt. J. of Aquatic Biolo. and Fish.

2016

The present study aims to describe the effect of heavy metals: copper, manganese, iron and zinc on the water quality of El-Bagouria Canal at Kafr El-Zayat region, El-Gharbiah Governorate. Seasonal variations of physico-chemical parameters were studied during the period from summer, 2013 to spring, 2014. Also, to study their effects on the different organs of the commercial fish species; Nile tilapia, Oreochromis niloticus by studying some of biochemical parameters of this fish species (total protein, total lipids, total carbohydrates, ASAT and ALAT) during the study period.Results revealed that, heavy metals in the water showed seasonal variations. Also, data exhibited the highest values of heavy metals in the different organs of O. niloticus were recorded during winter in the liver and kidney and the lowest were observed in the muscles. Biochemical analysis indicated devastating effects, especially enzymes activities, in the metabolic organs. Statistical analysis using analysis of variance (ANOVA, at p<0.05) based on data represent the measured values of heavy metals in the different organs of O. niloticus during the different seasons. One way analysis proved that, there were highly significant differences between the metals and between the different seasons also between different organs. On the other hand, two ways of ANOVA provided highly significant differences between organs and metals at the same seasons. While, non-significant differences were detected at the interactions between seasons and organs at the same metals and between seasons and metals at the same organs. One way analysis for biochemical parameters in the different organs of O. niloticus during the different seasons proved that, there were highly significant differences between the parameters. Meanwhile, there were significant differences between the different seasons and between target organs. Moreover, two ways of ANOVA provided highly significant differences between organs and parameters at the same seasons. While, non-significant differences were found at the interactions between seasons and organs at the same parameters and between seasons and parameters at the same organs.

Section: Zinc Concentrationsupporting

confidence: 67%

Influences of some pollutants on water quality of El-Bagouria Canal at Kafr El-Zayat Region, El-Gharbia Governorate, Egypt

Shehata

Egypt. J. of Aquatic Biolo. and Fish.

2016

Int. J. Adv. Res. Biol. Sci

“…Although, the concentration level of Mn detected in this study increased slightly during hotter seasons, it was still within the lower range of the permissible level of WHO (2004). Furthermore, Sudha Rani & Manikya Reddy (2003), Khaled (2004) and Osman (2012) testified the enrichment of this element in particulate matter due to the domestic and industrial waste inputs resulted in low dissolved oxygen content with H 2 S formation by bacterial. Zn and Cu concentrations of sediment samples detected in this stream were lower than permissible level of WHO (2004), and the lower concentrations of Zn might be due to leaching of heavy metals into the deeper layer of the soil and to the ground water (Gil-Díaz et al, 2014).…”

Section: Seasonal Variation Of Heavy Metals In Sedimentmentioning

confidence: 99%

Valuation of water pollution using enzymatic biomarkers in aquatic insects as bioindicators from El-Mansouriya stream, Dakahlia, Egypt

Bream¹,

Amer²,

Haggag³

et al. 2017

This investigation was aimed to evaluate water pollution with heavy metals using three biomarkers, as well as the study of the aquatic insects-seasons interaction. Three species of aquatic insects collected from El-Mansouriya stream (Dakahlia, Egypt), during the different seasons from spring 2014 to winter 2015. Water samples, sediments and whole body of the three aquatic insects were analyzed for heavy metal contents. As well as, two enzyme biomarkers, Acetylcholinesterase (AChE) and glutathione-S-transferase (GST) were used to monitor aquatic insects' response to water pollutants. Results showed various activities of the three biomarkers at the different studied seasons. Where, the concentration of selected heavy metals, in the whole body of the three insects, showed a relatively wide range for each metal analyzed. Also, higher values of metal pollution index (MPI) were attained in hotter seasons (spring and summer) and its decrements were observable during cooler seasons (autumn, winter), that provides a representative picture to the environmental state of environmental impacts on the aquatic ecosystem. While the heavy metals were found to be highly accumulated in selected aquatic insects body, as well as the variation of enzymes activity, thus both enzymes could be used as biomarker of water and sediment contamination.

African Journal of Aquatic Science

“…In order to evaluate the diverse effects of these complex chemical mixtures on aquatic organisms, there is a worldwide trend to complement chemical and physical parameters with biomarkers in aquatic pollution monitoring (Au 2004). The use of biomarkers has become an important tool for modern environmental assessments to predict pollutants involved in monitoring programmes (Osman et al 2010;Osman 2012;Badr et al 2014).…”

mentioning

confidence: 98%

“…While the quality of most of the Nile's water is within acceptable levels, there are several hot spots of pollution. Along the Damietta tributary, for example, industrial activities include the Talkha fertiliser plant, Kafersaad electric power station, Delta milk factory and Edfina preserved food factories; in addition, untreated sewage and domestic wastes are discharged from El-Serw and Ras El-Bar cities into the tributary (Abdo 2004;Osman 2012). For this reason, continuous monitoring of water quality parameters is necessary.…”

mentioning

confidence: 98%

“…Pollutants enter the organism through the gills and exert their primary toxic effects on the branchial epithelium (Playle et al 1992). Thus, changes in fish gills are among the most commonly recognised responses to environmental stressors and are indicative of physical and chemical stress (Au 2004;Osman 2012). The liver plays an important role in the vital functions of basic metabolism and is the major organ of accumulation, bio-transformation and excretion of contaminants in fish (Figueiredo-Fernandes et al 2006).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Histopathological biomarkers and genotoxicity in gill and liver tissues of Nile tilapiaOreochromis niloticusfrom a polluted part of the Nile River, Egypt

Baiomy

2016

Fish health is affected by water pollution. Oreochromis niloticus collected during summer 2014 from El-Serw, a polluted site on the Nile River, were compared with fish from a reference site, El-Zamalek. Histopathological changes were detected in gill and liver tissue samples using light and electron microscopy. In addition, the degree of DNA damage was measured using the comet assay. To indicate the severity of water pollution at the two sites, physico-chemical properties and heavy metal concentrations were investigated. Gill damage, including lamellar cell hyperplasia and aneurysm, was observed in the fish samples from the polluted site. The livers of fish from the polluted area showed necrosis and an increase in melanomacrophage centres. Histochemical results confirmed a marked rise of gill mucopolysaccharides and a reduction of carbohydrate stored in hepatocytes. Electron microscopy revealed clear alterations in gill and liver tissue of fish from the polluted site. The comet assay showed highly significant DNA damage in tilapia collected from the polluted site, compared to those from the reference site. Histopathological biomarkers and the comet assay may therefore be sensitive indicators of exposure to mixtures of aquatic pollutants in Nile tilapia.