Biology and Molecular Biology of Furoviruses

Brunt, A. A.; Richards, K.

doi:10.1016/s0065-3527(08)60581-3

Cited by 53 publications

(33 citation statements)

References 89 publications

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“…They are superficially similar to authentic tobamoviruses, but they differ in their relative instability in vitro, their low concentration in infected plants, and the transmission of most by plasmodiophoromycete fungi. The members which have been characterized have a bipartite genome (Koenig & Huth, 1988;Batista et al, 1989;Brunt & Richards, 1989). Additional RNAs have been identified in BNYVV in which although RNA 1 and RNA 2 are essential for infectivity, RNAs 3 and 4 seem to be required for an ability to infect sugar beet roots systemically (Lemaire et al, 1988;Koenig & Burgermeister, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Particle morphology (a helical capsid of 2.9 nm pitch, diameter of 18 nm) and coat protein Mr value 21400 (Randles et al, 1976) could place NVMV in either the tobamo-, hordei-, tobravirus (Matthews, 1982), or furovirus (fungus-transmitted labile rod-shaped viruses with a bipartite genome; Brunt & Shikata, 1986; 0000-9385 © 1990 SGM Richards, 1989;Brown, 1989) groups. However, no serological relationship to tobacco mosaic tobamovirus (TMV), beet necrotic yellow vein (BNYVV), soil-borne wheat mosaic (SBWMV), or potato mop top furoviruses, barley stripe mosaic hordeivirus (BSMV) or tobacco rattle tobravirus has been detected (Randles et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

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Nicotiana Velutina Mosaic Virus: Evidence for a Bipartite Genome Comprising 3 kb and 8 kb RNAs

Randles

Rohde

1990

Journal of General Virology

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DNA complementary to Nicotiana velutina mosaic virus (NVMV) RNA was 0oned and five segments larger than 0.9 kb were ~used in Northern blot hybridization analysis to identify two virus-specific RNAs, approximately 8 kb (RNA l) and 3 kb (RNA 2) in size. The clones selected as probes did not hybridize with RNA from various tobamoviruses, or from beet necrotic yellow vein (BNYVV) and peanut clump furoviruses. In an attempt to determine the taxonomic position of the virus, about 75 % of the NVMV RNA 2 was sequenced and four open reading frames (ORFs) were identified. ORFs 1, 2 and 3 encode proteins of Mr 20K, 39K and 13K, whereas ORF 4 was incomplete. ORFs 2, 3 and 4 overlapped in an arrangement closely resembling the triple gene block identified in BNYVV RNA 2, barley stripe mosaic virus (BSMV) RNA 2, potato virus X and potato virus M RNA. The presumed coat protein gene ofNVMV RNA 2 (ORF l) is situated to the 5' side of the triple gene block as for BNYVV and BSMV RNA 2. Amino acid homologies were detected among the 13K and 14K proteins of NVMV RNA 2, BNYVV RNA 2 and BSMV RNA 2. Significant homology was also detected between the 39K protein of NVMV RNA 2 and the 42K protein of BNYVV RNA 2, with a motif specific for ATP-and GTPbinding (NTP-binding motif), and a conserved viral DNA polymerase domain. The presence of a triple gene block in NVMV RNA 2 indicates that NVMV has affinities with members of the hordei-, furo-, potexand carlavirus groups but not with the tobamovirus group. The divided RNA genome of NVMV, and the sizes of the two RNAs suggest that NVMV is most closely allied to the furoviruses, but the unique nature of its different biological properties and lack of any serological relationships with furoviruses lead us to conclude that NVMV has no clear relatedness to any taxonomic group of plant viruses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nicotiana Velutina Mosaic Virus: Evidence for a Bipartite Genome Comprising 3 kb and 8 kb RNAs

Randles

Rohde

1990

Journal of General Virology

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“…After overwintering, infected plants start showing conspicuous mosaic symptoms on newly developing leaves, usually accompanied by stunting or rosetting of the plants when the temperature increases during the early and middle spring. Those plants that do not develop severe symptoms will recover from the disease and grow as vigorously as uninfected plants in the late spring and early summer (Brunt & Richards, 1989;Shirako & Wilson, 1999).…”

Section: Introductionmentioning

confidence: 99%

The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17 °C

Ohsato

Miyanishi

Shirako

2003

Journal of General Virology

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Systemic infection of wheat plants with Soil-borne wheat mosaic virus (SBWMV) requires temperatures below 20˚C. Here we examine the cause of the temperature sensitivity by inoculating infectious in vitro transcripts of SBWMV RNA1 and RNA2 to barley mesophyll protoplasts. After RNA inoculation, protoplasts were incubated at temperatures between 15 and 25˚C for up to 48 h. Western blot analysis showed that the capsid protein accumulated most abundantly at 17˚C but was not detectable at 25˚C. Northern blot analysis showed that the wild-type RNA1 and RNA2 and their subgenomic RNAs accumulated most abundantly at 17˚C but were barely detectable at 25˚C. An RNA1 mutant in which the p152 and p211 replicase genes were placed between the 59-and 39-untranslated regions also replicated most efficiently at 17˚C but not at 25˚C. Thus, the requirement for temperatures lower than 20˚C for SBWMV infection is primarily determined by replication of RNA1, which encodes the viral RNA replicase.

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“…Individual plants show the characteristic proliferation of fibrous roots around the tap root, "the root madness symptoms" of rhizomania. In severely infected plants, the tap root and lateral roots become necrotic and die then and the vascular tissue develops a pale brown coloration (Brunt & Richards, 1989).…”

Section: Rhizomania Is Caused By Beet Necrotic Yellow Vein Virus (Bnyvv)mentioning

confidence: 99%

Detection of Beet Necrotic Yellow Vein Virus by Double Stranded RNA Analysis

Kiliç

Yardımcı²,

Ürgen³

2013

IJB

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Beet necrotic yellow vein virus (BNYVV), the type member of the Benyvirus genus, has a multipartite, positive-sense single-stranded RNA genome, which consists generally of four, or in some isolates five, distinct RNA species. In this study, 108 BNYVV infected soil samples were collected from Isparta province, Turkey. Sugar beet plants cv Kasandra were grown in these soil samples using bait plant techniques and root samples were then analyzed by dsRNA analysis. The RNA was purified by CF-11 cellulose chromatography and gel electrophoresis. In 108 samples tested, dsRNA profiles were observed in 53 samples. No dsRNA bands were observed in negative control used in the analysis.

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Biology and Molecular Biology of Furoviruses

Cited by 53 publications

References 89 publications

Nicotiana Velutina Mosaic Virus: Evidence for a Bipartite Genome Comprising 3 kb and 8 kb RNAs

Nicotiana Velutina Mosaic Virus: Evidence for a Bipartite Genome Comprising 3 kb and 8 kb RNAs

The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17 °C

Detection of Beet Necrotic Yellow Vein Virus by Double Stranded RNA Analysis

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