The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17 °C

Ohsato, Shuichi; Miyanishi, M.; Shirako, Yukio

doi:10.1099/vir.0.19021-0

Cited by 39 publications

(24 citation statements)

References 12 publications

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“…By Western blot analysis, the largest amounts of CP and P1 were detected at 15 °C, followed by 17 °C, with lesser amounts at 12 °C and the lowest amounts at 20 °C (Fig. 2B), indicating that the optimal temperature for BaYMV RNA replication is 15 °C, corresponding closely to the optimum for disease manifestation in fields, and similar to the case of Soil‐borne wheat mosaic furovirus for which the optimal temperature for RNA replication is 17 °C (Ohsato et al. , 2003).…”

Section: Resultssupporting

confidence: 56%

“…Minorimugi and cv. Ryofu) and transfected with in vitro transcripts using a polyethyleneglycol (PEG) method, as described previously (Ohsato et al. , 2003).…”

Section: Methodsmentioning

confidence: 99%

“…Minorimugi and cv. Ryofu) and transfected with in vitro transcripts using a polyethyleneglycol (PEG) method, as described previously (Ohsato et al, 2003). Protoplasts were incubated at 15°C for 48 or 60 h and harvested for the detection of expressed viral proteins by Western blotting.…”

Section: In Vitro Transcription and Transfection To Barley Mesophyll mentioning

confidence: 99%

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Bymovirus reverse genetics: requirements for RNA2‐encoded proteins in systemic infection

You

Shirako

2010

Molecular Plant Pathology

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Barley yellow mosaic virus (BaYMV), the type species of the genus Bymovirus in the family Potyviridae in the picornavirus-like superfamily, causes a yellow mosaic disease of winter barley with significant yield losses in Europe and East Asia. Until now, infectious in vitro transcripts for the bipartite plus-sense RNA genome of any bymovirus species have not been available, rendering molecular analyses of bymovirus pathogenicity and the host resistance mechanisms difficult. In this study, we constructed the first cDNA clones of BaYMV RNA1 and RNA2, from which infectious RNA can be transcribed in vitro. Using in vitro transcripts, we showed that RNA1, which encodes eight proteins, including a viral proteinase NIa-Pro, the RNA-dependent RNA polymerase NIb, genome-linked viral protein VPg and the capsid protein CP, replicated autonomously in barley mesophyll protoplasts in the absence of RNA2 optimally at 15 degrees C, a temperature similar to the optimum for causing disease in barley fields. For systemic infection of barley plants, RNA1 alone was not sufficient and RNA2 was also required. Of the two proteins encoded on RNA2 (P1 with cysteine proteinase activity and P2 with unknown functions), P1 was essential and P2 was dispensable for systemic infectivity. The expression of both P1 and P2, but not the precursor polyprotein, together with RNA1 increased systemic infection and caused mosaic leaf symptoms. The infectious cDNA clones of BaYMV will be vital for future studies of bymovirus-host-vector interactions at the molecular level.

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Section: Resultssupporting

confidence: 56%

“…Minorimugi and cv. Ryofu) and transfected with in vitro transcripts using a polyethyleneglycol (PEG) method, as described previously (Ohsato et al. , 2003).…”

Section: Methodsmentioning

confidence: 99%

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Bymovirus reverse genetics: requirements for RNA2‐encoded proteins in systemic infection

You

Shirako

2010

Molecular Plant Pathology

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“…, Ohsato et al. ). Therefore, phenotyping in multiple years and locations is indispensable for reliable evaluation of SBCMV and WSSMV response.…”

mentioning

confidence: 98%

“…Efficient phenotyping for resistance to SBCMV and WSSMV is dependent on equal distribution of their vector as well as on its uniform load with the viruses on the trial site. In addition, both the transmission of the viruses by P. graminis (Slykhuis and Barr 1978) and the establishment or distribution of Furoand Bymoviruses in their host were described to be temperaturedependent (Slykhuis 1974, Drumm-Myers et al 1993, Ohsato et al 2003. Therefore, phenotyping in multiple years and locations is indispensable for reliable evaluation of SBCMV and WSSMV response.…”

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confidence: 99%

Oligogenic control of resistance to soil‐borne viruses SBCMV and WSSMV in rye (Secale cereale L.)

et al. 2016

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Rye production in European growing areas is constrained by the soilborne cereal mosaic virus (SBCMV) and the wheat spindle streak mosaic virus (WSSMV). To date, no European rye cultivars are known to exhibit resistance against these viruses. In this study, we pursued a quantitative trait locus (QTL) mapping strategy to identify genomic regions for resistance to SBCMV and WSSMV in rye. Three populations, each comprising 100 lines segregating for resistance to SBCMV and/or WSSMV, were evaluated for disease response at two years in three locations in Germany where soils are naturally infested with SBCMV and WSSMV. In the combined analysis across environments, one QTL for SBCMV resistance on chromosome 5R explained 31.9% of the phenotypic variation in one of the populations. For WSSMV resistance, one QTL explaining up to 64.0% of the phenotypic variation was detected on chromosome 7R in each of the three populations. On the Triticeae homoeologous group 5, we found evidence for synteny of the major QTL for SBCMV resistance between the wheat and rye genomes.

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Growth Conditions for Plant Virus–Host Studies

2009

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Plant virus replication and spread require specific interactions between the obligate organism, the virus, and its host, the plant. Both virus and plant factors, whether they are involved in virus accumulation or in defense against virus accumulation, require specific environmental conditions to maximize their activity. In order to understand how virus and plant factors function, it is therefore necessary to understand and control the environment that influences their activity. Temperature, light, moisture, air movement, soil status, and plant nutrition are the major environmental factors that influence a virus' accumulation in its host. This unit discusses each of these environmental factors, documenting their influence on virus accumulation in the plant, and discusses methods to best control and monitor their variation. Controlling the environment will limit experimental variation and lead to more meaningful and reproducible experimental results. Curr. Protoc. Microbiol. 14:16A.1.1‐16A.1.16. © 2009 by John Wiley & Sons, Inc.

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The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17 °C

Cited by 39 publications

References 12 publications

Bymovirus reverse genetics: requirements for RNA2‐encoded proteins in systemic infection

Bymovirus reverse genetics: requirements for RNA2‐encoded proteins in systemic infection

Oligogenic control of resistance to soil‐borne viruses SBCMV and WSSMV in rye (Secale cereale L.)

Growth Conditions for Plant Virus–Host Studies

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