Biologically active compounds from cyanobacteria extracts:in vivo and in vitro aspects

Carvalho, Luciana Retz de; Costa-Neves, Adriana; Conserva, Geanne A. Alves; Brunetti, Rafael Lanciani; Hentschke, Guilherme Scotta; Malone, Camila Francieli Silva; Torres, Luce Maria Brandão; Sant’Anna, Célia Leite; Rangel, Marisa

doi:10.1590/s0102-695x2013005000037

Cited by 14 publications

(17 citation statements)

References 17 publications

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“…The AEs of P. galeata CCIBt 3082 and G. splendidum CCIBt 3223 presented negative results [28,35]. As both strains are very frequent in our water reservoirs in Sao Paulo area, those where considered as of possible toxicological importance and mice toxicity experiments were conducted to detected the presence of other toxins.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

et al. 2014

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Cyanobacteria are common members of the freshwater microbiota in lakes and drinking water reservoirs, and are responsible for several cases of human intoxications in Brazil. Pseudanabaena galeata and Geitlerinema splendidum are examples of the toxic species that are very frequently found in reservoirs in Sao Paulo, which is the most densely populated area in Brazil. In the search for toxic strains collected from water reservoirs and maintained in the Cyanobacterial Culture Collection (CCIBt) of the Institute of Botany of Brazil, the acetic acid extracts (AE) of P. galeata CCIBt 3082 and G. splendidum CCIBt 3223 were analyzed by planar chromatography, which indicated the absence of cyanotoxins. Animal tests were then carried out, and both extracts were found to induce toxic effects in mice when administered intraperitoneally. The present study aimed to investigate whether the oral ingestion of the above mentioned cyanobacteria extracts would also induce toxic effects in mice. Necropsy and histopathological studies were conducted using tissue samples from the animals, which were euthanized one week after the administration of the extracts. The AE of P. galeata did not cause death but did induce transient symptoms, including eyebrow ptosis, straub tail, and pain. The euthanized animals presented hemorrhage in the liver, whereas the histological analysis showed disorganization of the hepatic parenchyma, necrosis, hyperemia, and proximity of the centrilobular vein in the liver. In addition, alterations in the convoluted tubules of the kidneys were observed, and the lungs were unaffected. The AE of G. splendidum caused only one death, and induced transient symptoms, such as dyspnea, paralysis, and pain, in the other mice. The necropsy of the euthanized mice showed hemorrhage in the lungs and liver. The lungs presented hemorrhagic focuses, alveolar collapse, and granulomatous foci. The liver presented hemorrhagic and enlarged sinusoids, hyperemia, proximity of the centrilobular vein, and disorganization of the hepatic parenchyma. Some areas also exhibited an inflammatory infiltrate and calcified tissue inside blood vessels. Necrosis and rupture of the convoluted tubule cells were observed in the kidneys. Further analysis of the both extracts indicated the lack of hemolytic activity, and the presence of two unknown anti-AChE substances in the AE of G. splendidum. Thus, P. galeata and G. splendidum are producers of novel toxins that affect mammals when administered orally.

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Section: Discussionmentioning

confidence: 99%

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

et al. 2014

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“…Chlorella vulgaris, Nostoc, and H. pluvialis and with high antimicrobial, anticoagulant, antiviral, antifungal, antienzymatic, anti-inflammatory, antioxidant, and antitumor activity [59][60][61][62][63]. Protoctists have a good ability to fold proteins into advanced three-dimensional structures.…”

Section: Veluchamy and Palaniswamymentioning

confidence: 99%

A Review on Marine Algae and Its Applications

Veluchamy

Palaniswamy²

2020

Asian J Pharm Clin Res

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The ocean is the major essential source of structurally unique natural products that are mainly present in living organisms. The essential products extracted from marine microbes and marine algae are highly analyzed areas in instinctive product research. Marine algae are the novel food with potential nutritional values used for multiple purposes in industry and medicine. They show pharmacological activities which are helpful for the invention of bioactive compounds. Furthermore, marine algae have shown to provide an abundant source of natural bioactive compounds with antidiabetic, anti-inflammatory, antiviral, antifungal, hypolipidemic, antioxidant, anti-hypercholesterolemia, antibacterial, and antineoplastic properties. They produce new secondary metabolites that possess biological activities and have the potential to be developed as therapeutic agents. Macroalgal lectins, fucoidans, kainoids, and other substances have been routinely used in the research of biomedical and also have biological activities. The potential pharmaceutical, medicinal, and research applications of these compounds are discussed.

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“…It has a specific locality of the fresh water system and does not bloom. It has been found that G. splendidum has a potential inhibitory effect on acetylcholinesterase activity [18].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Anti-Inflammatory Activity of Blue-Green Algae-Geitlerinema Splendidum Collected From Western Ghats, South India

Krishnaveni

Jency

2018

Asian J Pharm Clin Res

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Objective: Geitlerinema splendidum is non-edible blue-green algae available in freshwater systems. It belongs to Pseudanabaenacae genera. In exploring the medicinal properties of algae, our present work aimed to analyze the anti-inflammatory effect of the algae G. Splendidum.Methods: Anti-inflammatory activity was carried out by human red blood cell membrane stabilization assay for ethyl acetate and ethanol crude extracts of alga and found that the ethyl acetate extract has better inhibitory property than ethanol crude extract. Diclofenac was used as the standard drug. Results:The experimental results obtained are comparable to the reference drug and are found to be dose-dependent. Even at a minimum concentration of 12.5 µg/ml, the percentage inhibition was in the range of 56.6±0.12-58.5±0.03 %, which indicated that the alga G. splendidum has a satisfactory anti-inflammatory activity. Conclusion:Our present work is supposed to be the first report in exploring the anti-inflammatory effect of the algae G. splendidum.

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Biologically active compounds from cyanobacteria extracts:in vivo and in vitro aspects

Cited by 14 publications

References 17 publications

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

A Review on Marine Algae and Its Applications

In Vitro Anti-Inflammatory Activity of Blue-Green Algae-Geitlerinema Splendidum Collected From Western Ghats, South India

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