Biological responses of human bone mesenchymal stem cells to Ti and TiZr implant materials

Abstract

Background: Titanium-zirconium alloy (TiZr1317) is a new material used for biological implants.There are several studies on the effects of TiZr implants on the biological characteristics of human bone mesenchymal stem cells (hBMSCs).Purpose: The purpose of this study was to investigate the biological responses of hBMSCs to implant holes affected by the physicochemical properties of oral implants (Ti SLA , Ti SLActive , TiZr SLA , and TiZr SLActive ). Materials and Methods: Grade 4 Ti and TiZr (13-17% Zr) substrates were modified by sandblasted large-grit acid-etched (SLA) or hydrophilic sand-blasted large-grit acid-etched (SLActive), resulting in four types of surface with complex microstructures corresponding to the commercially-available implants SLA, RoxolidSLA, SLActive, and RoxolidSLActive (Institute Straumann AG, Basel, Switzerland). Physicochemical properties were detected and the biological responses of hBMSCs were observed.Results: Surface morphology characterization by scanning electron microscopy and atomic force microscopy revealed differences between the four groups. SLA ctive had higher surface energy/ wettability than SLA, indicating that increased surface energy/wettability can promote the absorption of osteogenic proteins and enhance osseointegration. hBMSCs seeded on SLActive substrates exhibited better performance in terms of cell attachment, proliferation and osteoblastic differentiation than cells seeded on SLA.

A review in titanium-zirconium binary alloy for use in dental implants: Is there an ideal Ti-Zr composing ratio?

“…On both Ti SLA and Ti-13–17 %Zr SLA surfaces, the osteoblastic differentiation and maturation markers of human MSCs and osteoblasts were enhanced to a comparable extent [3] . The SLActive Ti and Ti-13–17 %Zr surfaces exhibited increased cell attachment, proliferation, and osteoblastic differentiation compared to the SLA surfaces [4] . The long-term vertical osteoconductivity of SLActive Ti-Zr dental implants is comparable to those of Ti implants in rabbits [5] .…”

The Trends of TiZr Alloy Research as a Viable Alternative for Ti and Ti16 Zr Roxolid Dental Implants

“…Sandblasted, large grit, acid-etched TiZr dental implant surfaces enhanced hydrophilicity significantly, promoting better adsorption of certain proteins and osteoblastic differentiation and maturation of human bone mesenchymal stem cells (hBMSCs) [80][81][82][83]. The mRNA levels of bone morphogenic proteins (BMPs) were increased.…”

“…The mRNA levels of bone morphogenic proteins (BMPs) were increased. They are known to promote osteogenesis, which facilitates osteoblastic differentiation and maturation [83].…”

Construction of a Rough Surface with Submicron Ti2Cu Particle on Ti-Cu Alloy and Its Effect on the Antibacterial Properties and Cell Biocompatibility

“…Nedir et al reported that SLA treated surface was more conducive to the adhesion of osteoblasts compared with the acid-etched one [10,11]. Kaluderovic et al performed sandblasting acid etching on the surface of titanium and titanium alloys, and the results of in vitro experiments showed that Ti-SLA and TiZr-SLA had better osteogenic differentiation ability, significantly promoted the formation of bone nodules and cell mineralization, and accelerated the process of bone integration [12]. De Tullio et al implanted sandblasted and acid-etched titanium implants in sheep, and the results showed that the sandblasted and acid-etched titanium implants had higher binding strength with bone in a shorter healing time than mechanical titanium implants [13].…”