Biological properties of African swine fever virus Odintsovo 02/14 isolate and its genome analysis

Yelsukova, A. A.; Шевченко, И. В.; Варенцова, А. А.; Puzankova, O S; Zhukov, I. Yu.; Першин, А. С.; Ремыга, С. Г.; Zinyakov, N. G.; Mazloum, Ali; Vlasov, Ivan N.; Иголкин, А. С.; Lozovoy, D. A.; Груздев, К. Н.; Власова, Н. Н.

doi:10.25125/agriculture-journal-ijoear-oct-2017-15

Cited by 5 publications

(1 citation statement)

References 23 publications

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“…С целью выделения геномной ДНК (гДНК) использовали различные варианты вируса АЧС: исходный изолят Odintsovo 02/14 и 2 штамма адаптированного вируса ASFV/ARRIAH/CV-1/30 и ASFV/ARRIAH/ CV-1/50. Генетический материал выделяли фенолхлороформным методом, а осадок гДНК элюировали в воде, не содержащей нуклеаз [5] [6], поэтому данную его разновидность использовали для адаптации к росту в перевиваемой культуре фибробластоподобных псевдодиплоидных клеток африканской зелёной мартышки (Cercopithecus aethiops) (CV-1).…”

Section: материал и методыunclassified

Changes in the genome of African swine fever virus (Asfarviridae: Asfivirus: African swine fever virus) associated with adaptation to reproduction in continuous cell culture

Mazloum

Иголкин

Zinyakov

et al. 2021

Problems of Virology

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Введение. Возбудитель африканской чумы свиней (Suidae) (АЧС) -крупный (175-215 нм) двухцепочечный ДНК-вирус, относящийся к семейству Asfarviridae. К настоящему времени секвенирован и подробно проанализирован только геном штамма BA71V, адаптированного к клеточной культуре Vero. Целью данной работы явился сравнительный анализ полногеномного сиквенса исходного изолята вируса АЧС Odintsovo 02/14 и 2 штаммов, полученных на уровне 30 и 50 пассажей и адаптированных к росту в клеточной культуре CV-1. Материал и методы. В работе использованы различные варианты вируса АЧС: исходный изолят Odintsovo 02/14 и 2 штамма адаптированного вируса: ASFV/ARRIAH/CV-1/30 и ASFV/ARRIAH/CV-1/50. Библиотеку последовательностей конструировали с использованием набора «Nextera XT DNA library preparation kit» («Illumina», США). Результаты. Длина геномов штаммов ASFV/ARRIAH/CV-1/30 и ASFV/ARRIAH/CV-1/50 составила 186 529 и 186 525 п.н. соответственно. Всего между исходным и адаптированными вариантами обнаружены 78 однонуклеотидных полиморфизмов (single nucleotide polymorphisms, SNP); кроме того, установлено наличие крупноразмерной делеции величиной 2947 п.н. в правом (3'-концевом) вариабельном регионе у обоих адаптированных штаммов. Обсуждение. Возбудитель АЧС как ДНК-содержащий вирус может не иметь высокого мутационного статуса. Однако в данном исследовании повторно установлено, что адаптация этого инфекционного агента к росту в перевиваемой культуре клеток (КК) приводит к появлению крупноразмерной делеции в 3'-вариабельной области генома. Заключение. В связи с недостаточной изученностью данной проблемы необходимо проведение дополнительных исследований, что позволит подтвердить имеющиеся данные относительно влияния каждой из описанных мутаций на характер размножения вируса и степень его вирулентности.

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Section: материал и методыunclassified

Changes in the genome of African swine fever virus (Asfarviridae: Asfivirus: African swine fever virus) associated with adaptation to reproduction in continuous cell culture

Mazloum

Иголкин

Zinyakov

et al. 2021

Problems of Virology

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African swine fever virus – persistence in different environmental conditions and the possibility of its indirect transmission

Mazur-Panasiuk

Żmudzki

Woźniakowski

2019

Journal of Veterinary Research

150

120

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Since 2007, African swine fever (ASF) has posed a serious threat to the European swine industry. In Poland, the numbers of reported outbreaks in pigs and affected areas grow every year. In 2018, the disease was noted in Western Europe, in Belgium specifically, where several hundred infected wild boars have been detected so far. In 2018, the virus unexpectedly emerged in pig holdings in eastern China, northern Mongolia, Vietnam, and Cambodia, causing worldwide concern about its further spread. Since there is still no vaccine available, the only approach to control the disease is biosecurity. Identification of potential sources of the virus is extremely important in light of its phenomenal survivability. The review summarises the current knowledge about ASFV survivability and resistance to environmental conditions, and discusses the role of indirect contact in spreading the disease.

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Analysis of Changes in African Swine Fever Virus Genetic Structure and Biological Properties During Adaptation to Continuous Cell Culture

Mazlum

Зиняков

Першин

et al. 2019

Veterinariâ segodnâ

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Functions of many African swine fever virus genes and multigene family members have not been yet understood. In particular, no virus genes directly associated with pig virulence have been identifed. Identifcation of such genes will enable preparation of deletion mutant ASF virus strains as well as development and testing of pilot safe vaccines based on the said virus strains. Comparative analysis of the virus biological characteristics and detection of diﬀerences in its genome structure aﬀecting certain phenotypic features is a main method used for the virus basic pathogenicity and immunogenicity examination. The most interesting and eﬀective approach to addressing this problem is an analysis of changes in the gene structure during ASF virus adaptation to replication in continuous cell culture. The said factors have made continuous cell culture-adapted variant ASF virus preparation necessary. Variant viruses with modifed biological features were prepared during adaptation of ASFV Odintsovo 02/14 isolate to replication in CV-1 cell culture. Lethality level was 16.7% when pigs were infected with adapted variant virus at 30th passage and survived animals became resistant to reinfection with homologous virulent ASFV Arm07 isolate. It should be noted that the virus passage in non-permissive cell culture up to 30 serial passages did not result in changes in its genotype; however, a large 3,000 bp deletion similar to that one in continuous Vero-cell culture-adapted BA71V strain genome appeared in right terminal variable region of the genome.

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Biological properties of African swine fever virus Odintsovo 02/14 isolate and its genome analysis

Abstract: Abstract-We performed analysis of the biological properties of African swine fever virus (ASF) isolate

Cited by 5 publications

References 23 publications

Changes in the genome of African swine fever virus (Asfarviridae: Asfivirus: African swine fever virus) associated with adaptation to reproduction in continuous cell culture

Changes in the genome of African swine fever virus (Asfarviridae: Asfivirus: African swine fever virus) associated with adaptation to reproduction in continuous cell culture

African swine fever virus – persistence in different environmental conditions and the possibility of its indirect transmission

Analysis of Changes in African Swine Fever Virus Genetic Structure and Biological Properties During Adaptation to Continuous Cell Culture

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