Biological control of garlic (Allium) white rot disease using antagonistic fungi-based bioformulations

Abstract

White rot disease caused by Sclerotium cepivorum is a major yield reducing fungal disease of garlic found throughout the world, including Iran. The use of chemical fungicides is the most common control method for the disease at the present time. This control measure is costly, contaminates the environment, and harms non-target organisms. Moreover, since the pathogen is soil-borne, chemical control strategy is not quite effective against the disease. In this study, we tried to develop and prepare some new bioformulations based on three antagonistic fungal species: Trichoderma harzianum, T. asperellum, and Talaromyces flavus. Six isolates of the above-mentioned fungi were used along with the organic and inorganic carriers, rice bran and talc, to develop twelve new bioformulations. The effectiveness of the bioformulations were then evaluated in the control of garlic white rot disease in the greenhouse conditions in comparison with the healthy control, infected control, and the commonly used fungicide Carbendazim. The design of the experiment was completely randomised. There were 15 treatments each, with four replicates. The results of the greenhouse experiments indicated that almost all the developed bioformulations resulted in significant reductions (34.50 to 64.50%) in the incidence of white rot disease. In general, bioformulations which contained the organic carrier (rice bran) performed more effectively than those that contained the inorganic carrier (talc). Bioformulations which contained an organic carrier (rice bran) were as effective as the fungicide Carbendazim.

Systemic protection against pearl millet downy mildew disease induced by cell wall glucan elicitors from Trichoderma hamatum UOM 13

“…Our results corroborate earlier studies which have shown that the plant protecting ability of Trichoderma is by induced resistance and a wide range of Trichoder ma based formulations are commercially available which are known to induce resistance against a broad spectrum of plant pathogens in several crops (Woo et al 2006;Regliński et al 2012;Mahdizadehnaraghi et al 2015). Particularly, T. hamatum have been known to induce effective resistance against several plant pathogens.…”

Effect of inoculum density of Stromatinia cepivora on the amount of white rot reduced by Trichoderma species in garlic

“…Preparation of Trichoderma species inocula T. harzianum, T. koningii, and T. virens used in this study were grown on sterilized (121°C for 60 min) wheat bran as a carrier preparation (Mahdizadehnaraghi et al 2015). Carboxymethyl cellulose (1%) was used as an adhesive at the rate of 1:10 (v/w).…”

Trichoderma harzianum Inoculation Reduces the Incidence of Clubroot Disease in Chinese Cabbage by Regulating the Rhizosphere Microbial Community

“…Trichoderma is one of the most widespread biological agents currently used in agriculture to control diseases [ 18 , 19 , 20 , 21 , 22 , 23 , 24 ], being present in more than 60% of registered biological pesticides worldwide [ 25 , 26 , 27 ]. T. harzianum [ 28 , 29 ] was found to have a controlling effect on clubroot disease of Chinese cabbage in pot experiments [ 23 ].…”