Biological Control of Chickpea Fusarium Wilts Using Rhizobacteria “PGPR”

Zaim, Souad; Belabid, L.; Bayaa, B.; Bekkar, Ahmed Amine

doi:10.1007/978-981-10-0388-2_10

Cited by 8 publications

(7 citation statements)

References 86 publications

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“…The induction of systemic resistance in chickpea plants pre-challenged with soil-borne fungal pathogens by seed priming with bacterial endophytes was evaluated. Bacterial endophytes stimulate the expression of PR genes and phenol content and increase the activity of PR proteins such as PO, PPO, PAL, chitinases, lipoxygenases, and glucanases in the host plant as a defense mechanism to suppress the pathogenic effect ( Zaim et al, 2016 ; Bekkar et al, 2018 ; Kumari and Khanna, 2019 ). In the present study, we examined the accumulation of superoxide radicals, defense-related enzymes (PO and PAL), and the up-regulation of defense-related genes ( 60srp and IFR ) to evaluate bacterial endophytes for the induction of systemic resistance in chickpea plants pre-challenged with soil-borne fungal pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…Looking at the importance of the crops and the problem therein, biological control of these notorious pathogens is a viable and sustainable management option as chemical fungicides harm the environment and human health ( Sarma et al, 2015 ; Singh et al, 2020a ). In the recent past, several researchers have reported that plant growth promoting rhizobacteria (PGPR) mediates modulation of systemic resistance against wilt and root rot pathogens in many crops including chickpea ( Rudresh et al, 2005 ; Zaim et al, 2016 ; Bekkar et al, 2018 ; Kumari and Khanna, 2019 ). Besides PGPRs, bacterial endophytes are a group of bacteria that have a special ability to colonize and reside inside the plants and protect the host plant from biotic and abiotic stresses ( Ziedan, 2006 ; Sahu et al, 2020 , 2021 ; Singh et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Endophytic Bacillus subtilis antagonize soil-borne fungal pathogens and suppress wilt complex disease in chickpea plants (Cicer arietinum L.)

et al. 2022

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The present study aimed to identify potential endophytic bacteria antagonistic against three soil-borne fungal pathogens, Rhizoctonia solani, Sclerotium rolfsii, and Fusarium oxysporum f.sp. ciceri causing root rot, collar rot, and fungal wilt diseases in chickpea plants, respectively. A total of 255 bacterial endophytes were isolated from the leaves, stems, and roots of seven different crop plants (chickpea, tomato, wheat, berseem, mustard, potato, and green pea). The dual culture-based screening for antifungal properties indicated that three endophytic isolates had strong inhibition (>50%) against all three pathogens tested. Based on morphological, biochemical, and molecular characterization, the selected isolates (TRO4, CLO5, and PLO3) were identified as different strains of Bacillus subtilis. The bacterial endophytes (TRO4 and CLO5) were positive for plant growth promoting (PGP) traits viz., ammonia, siderophore, and indole-3-acetic acid (IAA) production. The bio-efficacy of the endophytes (TRO4, CLO5, and PLO3) was tested by an in planta trial in chickpea pre-challenged with R. solani, S. rolfsii, and F. oxysporum f.sp. ciceri. The B. subtilis strains TRO4 and CLO5 were found to be effective in reducing percent disease incidence (p ≤ 0.05) and enhancing plant growth parameters. The different root parameters viz. root length (mm), surface area (cm2), root diameter (mm), and root volume (cm3) were significantly (p ≤ 0.05) increased in TRO4 and CLO5 inoculated chickpea plants. Confocal Scanning Laser Microscopy showed heavy colonization of bacteria in the roots of endophyte-inoculated chickpea plants. The inoculation of endophytic Bacillus subtilis strains TRO4 and CLO5 in chickpea plants through seed biopriming reduced the accumulation of superoxide, enhanced the plant defense enzymes, and induced the expression of Pathogenesis-Related (PR) genes. Semi-quantitative analysis of defense-related genes showed differential activation of PR genes (60srp and IFR) by endophyte inoculation. The results of the present study reveal the antagonistic potential of B. subtilis strains TRO4 and CLO5 against three major soil-borne fungal pathogens and their ability to suppress wilt complex disease in chickpea plants. This is the first report on the simultaneous suppression of three major soil-borne fungal pathogens causing wilt complex in chickpea plants by endophytic B. subtilis strains.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endophytic Bacillus subtilis antagonize soil-borne fungal pathogens and suppress wilt complex disease in chickpea plants (Cicer arietinum L.)

et al. 2022

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“…They include anti-fungal secondary metabolites, sometimes termed antibiotics (e.g. fengycin, iturin, surfactin ( Chen et al., 2018 ), fusaricidin and polymyxin ( Zalila-Kolsi et al., 2016 )), as well as Volatile Organic Compounds (VOCs; Zaim et al., 2016 ; Legrand et al., 2017 ) ( Table 2 ). Extracellular lytic enzymes such as cellulase, chitinase, pectinase, xylanase ( Khan et al., 2018 ), protease and glucanase ( Saravanakumar et al., 2017 ), can also interfere with Fusarium growth or activity.…”

Section: Biocontrol Agents Against Fusarium and Th...mentioning

confidence: 99%

Microbial diversity in soils suppressive to Fusarium diseases

Todorović,

Moënne-Loccoz,

Raičević

et al. 2023

Front. Plant Sci.

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Fusarium species are cosmopolitan soil phytopathogens from the division Ascomycota, which produce mycotoxins and cause significant economic losses of crop plants. However, soils suppressive to Fusarium diseases are known to occur, and recent knowledge on microbial diversity in these soils has shed new lights on phytoprotection effects. In this review, we synthesize current knowledge on soils suppressive to Fusarium diseases and the role of their rhizosphere microbiota in phytoprotection. This is an important issue, as disease does not develop significantly in suppressive soils even though pathogenic Fusarium and susceptible host plant are present, and weather conditions are suitable for disease. Soils suppressive to Fusarium diseases are documented in different regions of the world. They contain biocontrol microorganisms, which act by inducing plants’ resistance to the pathogen, competing with or inhibiting the pathogen, or parasitizing the pathogen. In particular, some of the Bacillus, Pseudomonas, Paenibacillus and Streptomyces species are involved in plant protection from Fusarium diseases. Besides specific bacterial populations involved in disease suppression, next-generation sequencing and ecological networks have largely contributed to the understanding of microbial communities in soils suppressive or not to Fusarium diseases, revealing different microbial community patterns and differences for a notable number of taxa, according to the Fusarium pathosystem, the host plant and the origin of the soil. Agricultural practices can significantly influence soil suppressiveness to Fusarium diseases by influencing soil microbiota ecology. Research on microbial modes of action and diversity in suppressive soils should help guide the development of effective farming practices for Fusarium disease management in sustainable agriculture.

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“…Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is considered one of the major diseases of chickpea (Zaim et al, 2016). The pathogen causes wilting and yellowing of chickpea plants, resulting in losses of 10-40% of the annual crop depending on the variety and severity of the disease and can completely destroy crops if conditions are favorable for the disease development (Khan et al, 2014;Guerrero et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Toprak örneklerinden bakteri izolatlarının izolasyonu ve nohut solgunluğu hastalığına neden olan Fusarium oxysporum f. sp. ciceris’e karşı in vitro antagonistik potansiyellerinin belirlenmesi

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ENDES

2023

Harran Tarım Ve Gıda Bilimleri Dergisi

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In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates (YBUFoc9 and YBUFoc2) of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens (BM23 and BM40), 3 isolates as B. subtilis (BM8, BM32, and BM105), 6 isolates as B. cereus (BM10, BM69, BM70, BM104, BM111 and BM215), 2 isolates as B. megaterium (BM44 and BM135) and 1 isolate as B. pumilus (BM28). No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50% and from 0 to 58.33%, respectively. Among the 5 different species, B. amyloliquefaciens (BM23 and BM40) and B. subtilis (BM8, BM32, and BM105) showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates (BM23, BM40, BM8, BM32 and BM105) will be investigated and compared with approved fungicides for their fungicidal activity.

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Biological Control of Chickpea Fusarium Wilts Using Rhizobacteria “PGPR”

Cited by 8 publications

References 86 publications

Endophytic Bacillus subtilis antagonize soil-borne fungal pathogens and suppress wilt complex disease in chickpea plants (Cicer arietinum L.)

Endophytic Bacillus subtilis antagonize soil-borne fungal pathogens and suppress wilt complex disease in chickpea plants (Cicer arietinum L.)

Microbial diversity in soils suppressive to Fusarium diseases

Toprak örneklerinden bakteri izolatlarının izolasyonu ve nohut solgunluğu hastalığına neden olan Fusarium oxysporum f. sp. ciceris’e karşı in vitro antagonistik potansiyellerinin belirlenmesi

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