Endophytic Bacillus subtilis antagonize soil-borne fungal pathogens and suppress wilt complex disease in chickpea plants (Cicer arietinum L.)

Mageshwaran, Vellaichamy; Gupta, Rishabh; Singh, Shailendra; Sahu, Pramod Kumar; Singh, Udai B.; Chakdar, Hillol; Bagul, Samadhan Yuvraj; Paul, Surinder; Singh, Harsh Vardhan

doi:10.3389/fmicb.2022.994847

Cited by 18 publications

(8 citation statements)

References 49 publications

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“…Siderophores have a high affinity for Fe 3+ and are capable of binding Fe 3+ to form complexes, which helps Fe uptake by microorganisms, as well as being taken up by plants to increase iron content within the tissues and promote plant growth [ 41 ]. A study by Mageshwaran et al revealed that one of the factors that Bacillus subtilis TRO4 promotes in the growth of chickpea plants is the production of siderophores [ 42 ]. In line with previous reports, the ability of JK-25 to produce siderophores was verified in this study by the CAS blue agar plate method as one of the reasons for the ability to promote wheat growth.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Kang

Niu

Zhang

et al. 2023

Plants

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Common root rot caused by Bipolaris sorokiniana infestation in wheat is one of the main reasons for yield reduction in wheat crops worldwide. The bacterium strain JK-25 used in the current investigation was isolated from wheat rhizosphere soil and was later identified as Bacillus halotolerans based on its morphological, physiological, biochemical, and molecular properties. The strain showed significant antagonism to B. sorokiniana, Fusarium oxysporum, Fusarium graminearum, and Rhizoctonia zeae. Inhibition of B. sorokiniana mycelial dry weight and spore germination rate by JK-25 fermentation supernatant reached 60% and 88%, respectively. The crude extract of JK-25 was found, by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), to contain the surfactin that exerted an inhibitory effect on B. sorokiniana. The disruption of mycelial cell membranes was observed under laser scanning confocal microscope (LSCM) after treatment of B. sorokiniana mycelium with the crude extract. The antioxidant enzyme activity of B. sorokiniana was significantly reduced and the oxidation product malondialdehyde (MDA) content increased after treatment with the crude extract. The incidence of root rot was significantly reduced in pot experiments with the addition of JK-25 culture fermentation supernatant, which had a significant biological control effect of 72.06%. Its ability to produce siderophores may help to promote wheat growth and the production of proteases and pectinases may also be part of the strain’s role in suppressing pathogens. These results demonstrate the excellent antagonistic effect of JK-25 against B. sorokiniana and suggest that this strain has great potential as a resource for biological control of wheat root rot strains.

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Section: Discussionmentioning

confidence: 99%

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Kang

Niu

Zhang

et al. 2023

Plants

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“…Siderophores have a high affinity for Fe 3+ and are capable of binding Fe 3+ to form complexes, which make Fe easier for uptaking by microorganisms, as well as being taken up by plants to increase iron content within the tissues and promote plant growth [41]. A study by Mageshwaran et al revealed that one of the factors that Bacillus subtilis TRO4 promotes the growth of chickpea plants is the production of siderophores [42]. In line with previous reports, the ability of JK-25 to produce siderophores was verified in this study by the CAS blue agar plate method as one of the reasons for the ability to promote wheat growth.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Strain <em>Bacillus Halotolerans</em> JK-25 Mediate the Biocontrol of Wheat Common Root Rot Caused by <em>Bipolaris Sorokiniana </em>

Kang¹,

Zhang²,

Niu³

et al. 2023

Preprint

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Common root rot caused by Bipolaris sorokiniana infestation of wheat is one of the main reasons of yield reduction in wheat crops worldwide. In current study, strain JK-25 was isolated from soil of wheat rhizosphere and identified as Bacillus halotolerans based on morphological, physiological, biochemical characteristics and molecular identification. The strain showed significant antagonism to B.sorokiniana and broad-spectrum resistance to Fusarium oxysporum, Fusarium graminearum and Rhizoctonia zeae. Inhibition of Bipolaris sorokiniana mycelial dry weight and spore germination rate by JK-25 fermentation supernatant reached 60% and 88% respectively. The crude extract of JK-25 was found by MALDI-TOF-MS to contain the surfactin that exerted an inhibitory effect on B.sorokiniana. The disruption of mycelial cell membranes was observed under microscopy (LSCM) after treatment of B.sorokiniana mycelium with the crude extract. The antioxidant enzyme activity of B.sorokiniana was significantly reduced and the oxidation product MDA content increased after treatment with the crude extract. The incidence of root rot was significantly reduced in pot experiments with the addition of JK-25 culture ferment, which had a significant biological control effect of 72.06%. Its ability to produce siderophores may help to promote wheat growth, and the production of proteases and pectinases may also be part of the strain's role in suppressing pathogens. These results demonstrate the excellent antagonistic effect of JK-25 against B.sorokiniana and suggest that this strain has great potential as a resource for biological control of wheat root rot strains.

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“…It was established that the P. aeruginosa C10 strain has the highest bactericidal activity against pathogenic bacteria: Bacillus badius, B. mesentericus, B. mojavensis (Kadyrova et al, 2022). Mageshwaran et al (2022) investigated the antagonism of endophytic bacteria. 255 bacterial endophytes were isolated from the leaves, stems and roots of seven different cultivated plants (chickpea, tomato, wheat, bersem, mustard, potato and green peas).…”

Section: Discussionmentioning

confidence: 99%

Antifungal properties of rhizobacterial strains in relation to fungi of agricultural crops

Shakirov¹,

Mamanazarova²,

Sherembetov³

et al. 2022

Biosys. divers.

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On earth, pathogenic fungal infections are considered one of the most common crop problems, accounting for more than 80% of all plant diseases. The production of biologics and the fight against pathogens are highly relevant priorities. In this article, rhizosphere cultures isolated from wheat root tubers have been studied in detail for the development of pathogenic fungi. In the course of research, the inhibitory properties of rhizobacteria against common pathogenic fungi were studied. The main mechanism of antagonism is that bacteria directly affect phytopathogens or increase plant resistance to diseases by enhancing immunity. The antagonistic activity of rhizospheric microorganisms was studied and the level of their action was determined. It has been established that wheat rhizospheric bacteria Escherichia hermannii, Enterobacter cloacae and Rahnella aquatilis affect the development of pathogens that cause Alternaria and Fusarium wilt. In the experiments, 46 strains of rhizobacteria of the genus Enterobacter (strain E. cloacae CCIM1022), Rahnella (R. aquatilis CCIM1023), Pantoea, Pseudomonas, Bacillus, Escherichia were isolated and the effectiveness of their antifungal properties on the reproduction of pathogenic fungi was tested. Pathogenic fungi Alternaria, Fusarium, causing diseases of vegetable and melon crops, were isolated from host plants. It is noted that rhizobacteria have a negative impact and block the development of pure cultures of fungi isolated from tomatoes, zucchini, figs, melons, causing fungal diseases on cups. The 46 isolated and tested strains of rhizobacteria effectively inhibited the development of pathogenic fungi. Of these, Rahnella is the first bacterium studied for this purpose, and has been given great importance in experiments to block the development of Alternaria (A. alternata CCIM 1021). The causative agents of alternariosis are widely open. However, large-scale analysis of their presence and control of pathogens has always been difficult. In experiments to combat the pathogen in the laboratory, a new agent was used to spread nitrogen-fixing bacteria.

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Endophytic Bacillus subtilis antagonize soil-borne fungal pathogens and suppress wilt complex disease in chickpea plants (Cicer arietinum L.)

Cited by 18 publications

References 49 publications

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Antagonistic Strain <em>Bacillus Halotolerans</em> JK-25 Mediate the Biocontrol of Wheat Common Root Rot Caused by <em>Bipolaris Sorokiniana </em>

Antifungal properties of rhizobacterial strains in relation to fungi of agricultural crops

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