Biological and Phytochemical Screening of Plants

“…However, according to Farnsworth (1966), acid-fast bacilli, yeasts and fi lamentous fungi should be incorporated into the fi rst screening and, in order partially to fulfi ll this aim strains of Mycobacterium and of Candida were also included in our assays.…”

Section: Introductionmentioning

confidence: 99%

The antibiotic activity of some Brazilian medicinal plants

Lima¹,

Ximenes²,

Luna³

et al. 2006

Rev. bras. farmacogn.

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“…The yield of powder was 48% from water extracts, 34% from acetone and 21% from ethanol extracts for the root while 51%, 32% and 19% (w/w) were obtained for the stem bark, respectively. Farnsworth (1966), Eaton (1989), Silva et al (1993), Harborne (1998) and Houghton and Raman (1998).…”

Section: Preparation Of Extractsmentioning

confidence: 99%

“…Haemolysis test was further performed on the frothed extract in water to remove false positive results. Steroidal and triterpenes saponins were distinguished using the Liebermann-Burchard test (Farnsworth, 1966). The presence of flavonoids was determined using magnesium powder and a few drops of HCl test (Silva et al, 1993).…”

Section: Source Of Test Organismsmentioning

confidence: 99%

Antimicrobial Potency of Hydro, acetone and Ethanolic extracts of <i>Treculia africana</i> Decne Root and Stem Bark

Oyeyipo¹,

Onasoga²

2016

Journal of Applied Sciences and Environmental Management

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ABSTRACT:Treculia africana is a plant that is used in traditional medicine for the treatment of diverse ailments. To investigate the scientific basis for the use of the plant, the antimicrobial activities/potency of the root and stem bark extracts were evaluated against common gram negative and gram positive bacteria as well as yeast. The chemical constituents of the plant in addition to the effect of temperature and pH on its antimicrobial potencies were investigated. The phytochemical constituents of the dried powdered plant parts were extracted using aqueous and organic solvents (acetone and ethanol). The antimicrobial activity of the concentrated extracts was evaluated by determining the diameter of zone of inhibition against both gram negative and gram positive bacteria and yeast, using the disc diffusion technique. Phytochemical studies revealed the presence of glycosides, anthraquinone, free aglycone, saponin, steroids and polyphenols. The extracts were active against both gram positive and gram negative bacteria. The activities of the plant extracts were not affected at varied temperatures (4 o C, 30 o C, 60 o C and 100 o C), but decreased at alkaline pH. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the test organisms revealed lowest MIC and MBC against Salmonella paratyphi, Salmonella typhi and Bacillus subtilis with highest MIC and MBC exhibited against Staphylococcus aureus. Generally, there was significant difference (p<0.05) in the antimicrobial activities of the test extracts and control antibiotics, with the control antibiotics exerting broader antimicrobial activity. Treculia africana demonstrated broad spectrum antibacterial activity and a potential source of new classes of antibiotics that could be useful for infectious disease chemotherapy and control. ©JASEM

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Biological and Phytochemical Screening of Plants

Cited by 815 publications

References 348 publications

Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties

Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties

The antibiotic activity of some Brazilian medicinal plants

Antimicrobial Potency of Hydro, acetone and Ethanolic extracts of <i>Treculia africana</i> Decne Root and Stem Bark

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