Biological and Molecular Variability of <i>Zucchini yellow mosaic virus</i> in Iran*

Bananej, K.; Keshavarz, Tayyebeh; Vahdat, Aisan; Salekdeh, Ghasem Hosseini; Glasa, Miroslav

doi:10.1111/j.1439-0434.2008.01430.x

Cited by 17 publications

(9 citation statements)

References 21 publications

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“…Furthermore, ZYMV isolates from Serbia were characterized by two specifically conserved amino acid residues in the N-terminal part of the CP, as was previously reported for other isolates from Central Europe (Glasa et al 2007;Tóbiás and Palkovics 2003). Sequence diversity among ZYMV isolates has been reported in Australia (Coutts et al 2011b), Vietnam (Ha et al 2008) and Iran (Bananej et al 2008) with the greatest level of genetic diversity, including the deepest phylogenetic split, which has been seen in Asia (particularly China) (Simmons et al 2008). The lack of diversity has been reported among Central European isolates (Glasa et al 2007;Tóbiás and Palkovics 2003) and Martinique (Desbiez et al 1996) as well.…”

Section: Discussionmentioning

confidence: 69%

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

et al. 2012

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Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed.ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.

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Section: Discussionmentioning

confidence: 69%

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

et al. 2012

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“…The presence of ZYMV in Iran was first reported in 1988 from squash and muskmelon. Since then, ZYMV has spread to all vegetableproducing regions in Iran, making it the limiting factor in cucurbit crop production during summer and autumn, causing up to 100% yield loss (Benanej et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Towards the identification of retrotransposon-based and ISSR molecular markers associated with populations resistant to ZYMV in melon

Mandoulakani

Rahmanpour

Shaaf

et al. 2015

South African Journal of Botany

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Edited by E BalazsKeywords: Cucumis melo L. Zucchini yellow mosaic virus IRAP REMAP Mixed linear model Melon (Cucumis melo L.) is one of the most important commercial vegetable crops grown in Iran. Very few studies on the genetic structure and diversity of melon landraces of Iran are available in the literature. In addition, it is susceptible to a number of diseases causing reduction in both productivity and quality of the crop. In this study we applied inter retrotransposon amplified polymorphism (IRAP), retrotransposon-microsatellite amplified polymorphism (REMAP) and inter simple sequence repeat (ISSR) molecular markers to investigate genetic diversity and its association with populations resistant to ZYMV in 11 Iranian melon populations and 12 commercial lines. Significant differences (P ≤ 0.01) were detected among populations for reaction to ZYMV infection. No symptoms observed on inbred line PI414723, while variety Charentais T showed the highest value of virus infection (1.87). Based on five IRAP, 15 REMAP and 20 ISSR primers, a total of 656 reproducible and scorable loci were generated, among them 75.41% were polymorphic. Population structure analysis using STRUCTURE software identified K = 10 as the reliable value for the number of clusters. Associations between molecular markers and populations resistant to ZYMV were tested using mixed linear model (MLM) approach that accounts for population structure. Highly significant associations were found for seven REMAP and four ISSR markers affecting ZYMV resistance (P ≤ 0.01). This study provides evidence that using natural germplasms, the diversity can be enhanced for marker-trait associations that can be validated in segregating populations and exploited through marker-assisted selection.

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“…than 50 well characterized viruses are known to naturally infect cucurbits (Kucharek & Purcifull, 1997). Among them, aphid-borne viruses are considered economically the most important cucurbit pathogens worldwide (Provvidienti & Schroeder, 1970;Lecoq et al, 2003;Bananej et al, 2008). On cucurbits in Serbia, five viruses have been identified: Zucchini yellow mosaic virus (ZYMV), Cucumber mosaic virus (CMV), Watermelon mosaic virus (WMV), Tomato spotted wilt virus (TSWV) (Vučurović et al, 2012), and Cucurbit aphid-borne yellows virus (CABYV) (Vučurović et al, 2011).…”

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confidence: 99%

Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia

Vučurović¹,

Petrović-Obradović²,

Radonjić³

et al. 2018

Ratar i povrt

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The fauna of aphids appearing on cucurbits, including oilseed pumpkin have been poorly investigated in Serbia. Yellow water traps were used to determine the diversity of aphid species visiting cucurbits in Serbia and to monitor their flight activity. During the years 2009 to 2011, a total of 1,447 specimens were collected and a total of 57 different aphid taxa were identified. In 2009, the highest total number of aphids were caught (755), followed by 203 and 489 aphids in 2010 and 2011, respectively. The most abundant species were Aphis fabae (15%), Myizus persicae (13.4%) and Acyrthosiphon pisum (11.7%), followed by Anoecia corni (6.4%) and Aphis gossypii (5.6%). All of them, except A. corni, are reported as efficient vectors of cucurbit viruses. These five species represent 52.1% of all aphid species collected within this study. Out of 57 taxa found in oilseed pumpkin crops, 17 are known as vectors of ZYMV, 18 of WMV and 15 of CMV. Generally, 25 out of a total of 57 taxa are known as vectors of at least one of three viruses present in Serbia. From total of 1,447 specimens collected during three years of investigation, 917 (63.4%) are vectors of at least one of three viruses. Individual oilseed pumpkin plants were frequently colonized by A. gossypii during all three years of investigation. Results of this study showed that Serbian oilseed pumpkin growing localities are grouped among medium to reach in aphidofauna, according to total Shannon-Weaver index values which varied from 1.8 to 2.8. The highest value of Shannon-Weaver (2.8) was recorded in the Bački Petrovac I locality in 2009, while the lowest Shannon-Weaver (1.8) was recorded in Kisač locality in 2010. The maximum values of Shanon-Weaver diversity index in almost all crops were recorded in the second week of investigation or in early stages of oilseed pumpkin growth when plants had 5-6 true leaves or were in flowering stage, when potential virus infection could lead to significant yield losses.

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Biological and Molecular Variability of Zucchini yellow mosaic virus in Iran*

Cited by 17 publications

References 21 publications

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates

Towards the identification of retrotransposon-based and ISSR molecular markers associated with populations resistant to ZYMV in melon

Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia

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