Towards the identification of retrotransposon-based and ISSR molecular markers associated with populations resistant to ZYMV in melon

Abstract

Edited by E BalazsKeywords: Cucumis melo L. Zucchini yellow mosaic virus IRAP REMAP Mixed linear model Melon (Cucumis melo L.) is one of the most important commercial vegetable crops grown in Iran. Very few studies on the genetic structure and diversity of melon landraces of Iran are available in the literature. In addition, it is susceptible to a number of diseases causing reduction in both productivity and quality of the crop. In this study we applied inter retrotransposon amplified polymorphism (IRAP), retrotransposon-microsatellite amplified polymorphism (REMAP) and inter simple sequence repeat (ISSR) molecular markers to investigate genetic diversity and its association with populations resistant to ZYMV in 11 Iranian melon populations and 12 commercial lines. Significant differences (P ≤ 0.01) were detected among populations for reaction to ZYMV infection. No symptoms observed on inbred line PI414723, while variety Charentais T showed the highest value of virus infection (1.87). Based on five IRAP, 15 REMAP and 20 ISSR primers, a total of 656 reproducible and scorable loci were generated, among them 75.41% were polymorphic. Population structure analysis using STRUCTURE software identified K = 10 as the reliable value for the number of clusters. Associations between molecular markers and populations resistant to ZYMV were tested using mixed linear model (MLM) approach that accounts for population structure. Highly significant associations were found for seven REMAP and four ISSR markers affecting ZYMV resistance (P ≤ 0.01). This study provides evidence that using natural germplasms, the diversity can be enhanced for marker-trait associations that can be validated in segregating populations and exploited through marker-assisted selection.

Agro-Morphological Variation in Some Iranian Melon (Cucumis melo L.) Genotypes Revealed by Multivariate Analysis

“…Shadegani was different than inodorous group mainly based on aroma and abscission of peduncle and Magasii was different than cantaloupensis group based on rind netting and soluble solids content. In previous studies, Shadegani was clustered as an inodorous local cultivar (Abdollahi et al, 2015). The discrepancies between Shadegani and the inodorous types might be due to the differences in cultivation history and adaptation of populations to local conditions and also the high rate of out crossing in melon landraces and cultivars, which causes gene flow between populations and lose of characteristic traits among them.…”

“…Genetic diversity in different genotypes of melon has been analyzed worldwide, based on various techniques such as assessment of morphological traits (Laghetti et al 2008;Escribano and Lazaro 2009), molecular markers such as isozymes (McCreight et al 2004), amplified fragment length polymorphism (AFLP) (Yashiro et al 2005), random amplified polymorphic DNA (RAPD) (Sensoy et al 2007;Yi et al 2009) and simple sequence repeat (SSR) (Hanane et al 2015). There are also a few studies on the genetic structure of Iranian melon (Abdollahi et al 2015;Danesh et al 2015;Kohpayegani and Behbahani 2008;Nasrabadi et al 2012;Raghami et al 2014); But there are still many accessions, landraces and improved cultivars remaining to be studied.…”

Search of effective IRAP markers for sakura genotyping

“…In turn, the IRAP genotyping method is easy to implement and not resource-demanding. The above-mentioned features of this method make it a convenient approach in genetic studies of cultures at the initial stage [11][12].…”