Biological activities and chemical characterization of the Lebanese endemic plant <i>Origanum ehrenbergii</i> Boiss

Dawra, Michella; Rayess, Youssef El; Beyrouthy, Marc El; Nehme, Nancy; Hage, Rawad El; Taillandier, Patricia; Bouajila, Jalloul

doi:10.1002/ffj.3646

Cited by 15 publications

(15 citation statements)

References 21 publications

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“…The antioxidant scavenging activity was studied using 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), as described by Blois, [ 39 ] with some modifications. In a 96-well microplate (Micro Well, Thermo Fisher Scientific, Illkirch France), 20 μL of the diluted plant extract (0.5 mg/mL) was added to 180 μL of 0.2 mM methanolic DPPH solution.…”

Section: Methodsmentioning

confidence: 99%

Study on the Chemical Composition and the Biological Activities of Vitis vinifera Stem Extracts

et al. 2022

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Vitis vinifera (V. vinifera) is a herbaceous plant, cultivated worldwide and known for its biological benefits. The aim of this study is the investigation of the chemical composition as well as the determination of the biological potential of different grape stem extracts obtained by maceration and accelerated solvent extraction (ASE). The HPLC analysis of the tested extracts led to the identification of 28 compounds of which 17 were identified for the first time in grape plants, in addition to seven revealed in the stem part for the first time. Twenty-nine volatile molecules have been detected by GC-MS in the grape stem part; among them seven were identified for the first time in the grape plant. For the biological analysis, the ethyl acetate extract (EtOAc) obtained by maceration showed a significant potential regarding antioxidant activity (IC50 = 42.5 µg/mL), anti-Alzheimer (IC50 = 14.1 µg/mL), antidiabetic (IC50 = 13.4 µg/mL), cytotoxic with HCT-116 (IC50 = 12.5 µg/mL), and anti-inflammatory (IC50 = 26.6 µg/mL) activities, as well as showing the highest polyphenol content (207.9 mg GAE/g DW).

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Section: Methodsmentioning

confidence: 99%

Study on the Chemical Composition and the Biological Activities of Vitis vinifera Stem Extracts

et al. 2022

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“…The collected aerial parts of S. fruticosa were dried in shade at an ambient temperature and transformed into powder with a particle size of 0.8 mm [ 29 ]. A cold maceration with four solvents presenting an increasing polarity (CHX, DCM, EtOAc and MeOH) was performed to yield the four organic extracts.…”

Section: Methodsmentioning

confidence: 99%

“…The total phenolic content (TPC) of each extract was evaluated spectrophotometrically at 765 nm using the Folin–Ciocalteu (FC) method as described by Dawra et al [ 29 ]. Gallic acid (0–115 μg/mL) was used for the calibration curve.…”

Section: Methodsmentioning

confidence: 99%

Chemical Characterization and Antioxidant, Antibacterial, Antiacetylcholinesterase and Antiproliferation Properties of Salvia fruticosa Miller Extracts

et al. 2023

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The Salvia fruticosa (Mill.) is the most medicinal plant used in Lebanon. The aim of this study is to investigate the phytochemical composition and the biological activities (in vitro) of its extracts. The plant was extracted by cold maceration with four solvents presenting an increasing polarity: cyclohexane (CHX), dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH). The extracts were screened for their chemical composition by a HPLC-DAD detector for phenolic compounds identification and quantification and by GC-MS for volatile compounds detection. The antioxidant capacity (DPPH inhibition) was tested. Biological activities, mainly anti-Alzheimer activity (acetylcholinesterase inhibition), the antiproliferation of two human colon cancer cell lines (HCT-116 and Caco-2 cells) and antibacterial activity, were evaluated. Ten aromatic compounds were quantified by HPLC-DAD analysis. A total of 123 compounds were detected by GC-MS analysis. The MeOH extract showed a very interesting antioxidant activity with an inhibition percentage (IP) of 76.1% and an IC50 of 19.4 μg/mL. The EtOAc extract exhibited the strongest inhibition against the acetylcholinesterase activity (IP = 60.6%) at 50 μg/mL. It also strongly inhibited the proliferation of the HCT-116 cells (IP = 87.5%), whereas the DCM extract gave the best result with the Caco-2 cells (IP = 72.3%). The best antibacterial activity was obtained with the MeOH extract against Staphylococcus aureus (MIC = 1.2 μg/mL) and with the EtOAc extract against Escherichia coli (MIC = 2.4 μg/mL). This study highlights the chemical composition and therapeutic potential of S. fruticosa. It is important to mention that the following chemical compounds were identified for the first time in plant extracts: 2,6,11,15-tetramethyl-hexadeca-2,6,8,10,14-pentaene; 4,5,6,7-tetrahydroxy-1,8,8,9-tetramethyl-8,9-dihydrophenaleno [1,2-b]furan-3-one; podocarpa-1,8,11,13-tetraen-3-one,14-isopropyl-1,13-dimethoxy; podocarpa-8,11,13-trien-3-one,12-hydroxy-13-isopropyl-,acetate; 3′,8,8′-trimethoxy-3-piperidin-1-yl-2,2′-binaphthyl-1,1′,4,4′-tetrone; and 2,3-dehydroferruginol, thus underlining the originality of this study.

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“…This test is based on the reduction of MTT (a yellow water-soluble tetrazolium salt) by the mitochondrial dehydrogenase of intact cells to generate purple formazan. The anti-proliferation activity of the extracts against HCT-116 cells (a human colon cancer cell line) was estimated with a modified version of the protocol described by Dawra et al [34]. The cells were purchased from ATCC, and cultured in RPMI 1640 medium at 37 °C in a humidified incubator (Thermo Fischer Scientific, Illkirch, France) under an atmosphere containing 5% CO 2 .…”

Section: Cell Viability Assay With Hct-116 Cellsmentioning

confidence: 99%

In Vitro Bioactivities of Extracts from Tomato Pomace

Jamaleddine¹,

de²,

Bouajila³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Tomato pomace (TP) is a coproduct generated by the extraction of tomato pulp, and is a potential source of bioactive molecules. In this study, we isolated several fractions from TP and evaluated their biological properties. Materials and Methods: TP was treated by maceration at room temperature with green solvents (ethanol, ethyl acetate, ethanol:water and ethanol:ethyl acetate) or supercritical CO2 (SC-CO2). The extracts were analyzed by HPLC-DAD to determine their composition, and their antioxidant activity was assessed. The potential therapeutic effects of the isolated fractions were assessed in vitro. Results: We identified 30 molecules on chromatography profiles, which revealed an abundance in phenolic acids, carotenoids, flavonoids and tannins, with differences in selectivity according to the solvent and pretreatment used. The highest radical scavenging activities were measured at 64-72% inhibition, corresponding to the ethanol or ethanol:water extracts with the highest polyphenol or flavonoid contents. Carotenoid content was increased by chemical pretreatment, to attain levels of 161 mg β-carotene/g ethyl acetate extract. This level of carotenoids seemed to have anti-inflammatory effects, with an IC50 of 9.3 µg/mL. In terms of anti-diabetic effects, the activities of α-glucosidase and α-amylase were best inhibited by extraction in an ethanol-to-water mixture (50:50). Cytotoxicity in a tumor cell line were highest for SC-CO2 extracts (64.5% inhibition) and for ethanol extracts obtained after the enzymatic pretreatment of TP (37% inhibition). Some extracts also had dose-dependent activity against Zika virus. Conclusions: New fractions obtained from TP with ecocompatible solvents in mild conditions are rich in bioactive molecules. A comparison of the chromatographic profiles of the extracts led to the identification of several key molecules with therapeutic properties. The chemical pretreatment of TP is justified as a mean of increasing the carotenoid content of ethyl acetate fractions, whereas enzymatic pretreatment can increase the antioxidant activity of ethyl acetate fractions and increase the cytotoxicity of ethanol fractions. The SC-CO2 fraction contained a smaller number of metabolites detectable on HPLC, but it had high levels of cytotoxicity and antioxidant activity. Finally, the fractions obtained appeared to be suitable for use to target one or several of the biological activities studied.

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Biological activities and chemical characterization of the Lebanese endemic plant Origanum ehrenbergii Boiss

Cited by 15 publications

References 21 publications

Study on the Chemical Composition and the Biological Activities of Vitis vinifera Stem Extracts

Study on the Chemical Composition and the Biological Activities of Vitis vinifera Stem Extracts

Chemical Characterization and Antioxidant, Antibacterial, Antiacetylcholinesterase and Antiproliferation Properties of Salvia fruticosa Miller Extracts

In Vitro Bioactivities of Extracts from Tomato Pomace

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