Bioisomerization kinetics of γ-HCH and biokinetics of Pseudomonas aeruginosa degrading technical HCH

Lodha, Bharat; Bhat, Praveena; Kumar, Manish; Vaidya, Atul N.; Mudliar, Sandeep N.; Killedar, Deepak J.; Chakrabarti, Tapan

doi:10.1016/j.bej.2006.12.015

Cited by 31 publications

(14 citation statements)

References 47 publications

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“…Two possible reasons may be (a) drop in pH and (b) deficit in availability of oxygen. This phenomenon is also reported by various researchers during batch degradation studies for toxic organics (Kumar et al 2005;Arutchelvan et al 2006;Lodha et. al.…”

Section: Morphological and Bio-chemical Characteristics Of Isolated Csupporting

confidence: 52%

“…2 Effect of initial pyridine concentration on the pyridine degradation by isolated pyridine degrading consortium/strain on a semi logarithmic graph as mentioned by Park et al (2002), Kumar et al (2005), and Lodha et al(2007). The lag phase was observed in the initial stage of the growth curve, thereafter linear increment in the biomass concentration was observed (exponential phase) at all initial pyridine concentrations, which indicated that the pyridine was the limiting substrate in this region and the culture growth was exponentially.…”

Section: Evaluation Of Growth Kinetics Of Isolated Consortium/strainmentioning

confidence: 96%

“…The same trend has been reported for other toxicants like phenol, catechol, technical HCH, TNT, etc. (Kumar et al 2005;Lodha et al 2007;Park et al 2002). The specific growth rates were 0.0015 h -1 at 1 mg l -1 , and 0.01233 h -1 at 50 mg l -1 .…”

Section: Evaluation Of Growth Kinetics Of Isolated Consortium/strainmentioning

confidence: 99%

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Biodegradation of pyridine by an isolated bacterial consortium/strain and bio-augmentation of strain into activated sludge to enhance pyridine biodegradation

et al. 2008

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Pyridine and pyridine based products are of major concern as environmental pollutants due to their recalcitrant, persistent, toxic and teratogenic nature. In this study, we describe biodegradation of pyridine by an isolated consortium/strain under aerobic condition. Batch experiment results reveal that at lower initial pyridine concentrations (1-20 mg l(-1)), almost complete degradation was observed whereas at higher concentration (30-50 mg l(-1)), the degradation efficiency was dropped significantly. This may be due to inhibitory effect of pyridine at higher concentrations. The value of decay and yield coefficient was also determined. Furthermore, the bio-augmentation of isolated consortium/strain into the activated sludge consortium in different quantity has been also done and the effect of bio-augmentation on degradation has been studied. The results reveal that as the quantity of bio-augmentation increases, the degradation of pyridine increases. At 25% bio-augmentation, complete degradation of 20 mg l(-1) of pyridine can be achieved within 96 h of incubation. Thus, the study concluded that the bio-augmentation of the isolated consortium/strain into the sludge enhances the pyridine degradation efficiency of the biomass.

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Section: Morphological and Bio-chemical Characteristics Of Isolated Csupporting

confidence: 52%

Section: Evaluation Of Growth Kinetics Of Isolated Consortium/strainmentioning

confidence: 96%

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Biodegradation of pyridine by an isolated bacterial consortium/strain and bio-augmentation of strain into activated sludge to enhance pyridine biodegradation

et al. 2008

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“…Biotic isomerization of different compounds have also been reported, such as bioisomerization of c-Hexachlorocyclohexane (Lindane) to a-Hexachlorocyclohexane in bottles of sediment and seawater (Benezet and Matsumur, 1973) or by P. aeruginosa (Lodha et al, 2007) and reciprocal bioisomerization of butyrate and isobutyrate by triculture (Wu et al, 1994) or by Strain WoG13 in anerobic conditions (Matthies and Schink, 1992). However, little is known about the isomerization of pyrethroids by biotic effects.…”

Section: Isomerization Of Beta-cp By Strain Ch7mentioning

confidence: 95%

Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production

Zhang

Wang

Yan

2011

Bioresource Technology

144

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“…The kinetic study of degradation mediated by the reported isolates DAB-1Y and DAB-1W, in batch culture, was carried out by the method described by Lodha et al (2007) with slight modification. The whole cells from the culture grown in MSM broth were used as source enzyme released in the culture supernatant and used to study γ-HCH degradation.…”

Section: Methodsmentioning

confidence: 99%

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Kumar

Sharma

2016

Bioresour. Bioprocess.

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BackgroundThis study was carried out to isolate and characterize the bacterial strains from lindane-contaminated soil and they were also assessed for their lindane-degrading potential.MethodsIn this study the enrichment culture method was used for isolation of lindane degrading bacterial isolates, in which the mineral salt medium (MSM) supplemented with different concentrations of lindane was used. Further, the screening for the potential lindane degrading isolates was done using the spray plate method and colorimetric dechlorinase enzyme assay. The selected isolates were also studied for their growth response under varying range of temperature, pH, and NaCl. The finally selected isolates DAB-1Y and DAB-1W showing best lindane degradation activity was further subjected to biochemical characterization, microscopy, degradation/kinetic study, and 16S rDNA sequencing. The strain identification were performed using the biochemical characterization, microscopy and the species identifies by 16S rDNA sequence of the two isolates using the standard 16S primers, the 16 S rRNA partial sequence was analyzed through BLAST analysis and phylogenetic tree was generated based on UGPMA clustering method using MEGA7 software. This shows the phylogenetic relationship with the related strains. The two isolates of this study were finally characterized as Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W, and their 16S rRNA sequence was submitted to GenBank database with accession numbers, KJ811539 and KX986577, respectively.ResultsOut of the 20 isolates, the isolates DAB-1Y and DAB-1W exhibited best lindane-degrading activity of 94 and 98%, respectively, recorded after 8 days of incubation. The optimum growth was observed at temperature 30 °C, pH 7, and 5% NaCl observed for both isolates. Of the four isomers of hexachlorocyclohexane, isomer α and γ were the fastest degrading isomers, which were degraded up to 86 and 94% by isolates DAB-1Y and up to 93 and 98% by DAB-1W, respectively, reported after 8 days incubation. Isomer β was highly recalcitrant in which maximum 35 and 32% lindane degradation was observed even after 28 days incubation by isolates, DAB-1Y and DAB-1W, respectively. At lower lindane concentrations (1–10 mg/L), specific growth rate increased with increase in lindane concentration, maximum being 0.008 and 0.006/day for DAB-1Y and DAB-1W, respectively. The 16 S rRNA partial sequence of isolate DAB-1Y showed similarity with Kocuria sp. by BLAST analysis and was named as Kocuria sp. DAB-1Y and DAB-IW with Staphylococcus sp. DAB-1W. The 16S rDNA sequence of isolate DAB-1Y and DAB-1W was submitted to online at National Centre of Biotechnology Information (NCBI) with GenBank accession numbers, KJ811539 and KX986577, respectively.ConclusionsThis study has demonstrated that Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W were found efficient in bioremediation of gamma-HCH and can be utilized further for biodegradation of environmental contamination of lindane and can be utilized in bioremediation program.Electronic supplement...

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Bioisomerization kinetics of γ-HCH and biokinetics of Pseudomonas aeruginosa degrading technical HCH

Cited by 31 publications

References 47 publications

Biodegradation of pyridine by an isolated bacterial consortium/strain and bio-augmentation of strain into activated sludge to enhance pyridine biodegradation

Biodegradation of pyridine by an isolated bacterial consortium/strain and bio-augmentation of strain into activated sludge to enhance pyridine biodegradation

Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

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