Biodegradation of pyridine by an isolated bacterial consortium/strain and bio-augmentation of strain into activated sludge to enhance pyridine biodegradation

Abstract

Pyridine and pyridine based products are of major concern as environmental pollutants due to their recalcitrant, persistent, toxic and teratogenic nature. In this study, we describe biodegradation of pyridine by an isolated consortium/strain under aerobic condition. Batch experiment results reveal that at lower initial pyridine concentrations (1-20 mg l(-1)), almost complete degradation was observed whereas at higher concentration (30-50 mg l(-1)), the degradation efficiency was dropped significantly. This may be due to inhibitory effect of pyridine at higher concentrations. The value of decay and yield coefficient was also determined. Furthermore, the bio-augmentation of isolated consortium/strain into the activated sludge consortium in different quantity has been also done and the effect of bio-augmentation on degradation has been studied. The results reveal that as the quantity of bio-augmentation increases, the degradation of pyridine increases. At 25% bio-augmentation, complete degradation of 20 mg l(-1) of pyridine can be achieved within 96 h of incubation. Thus, the study concluded that the bio-augmentation of the isolated consortium/strain into the sludge enhances the pyridine degradation efficiency of the biomass.

Microbial degradation of pyridine and α-picoline using a strain of the genera Pseudomonas and Nocardia sp.

“…This high rate of degradation may be attributed to cultivation of the granules at a phenol concentration of 500 mg L -1 [1]. Pyridine biodegradation by an isolated consortium in the concentration range of 1-20 mg L -1 is reported [18] wherein the authors have evaluated the growth kinetics and have derived kinetic constant values for the isolated consortium viz. Y: 0.155 g biomass/g pyridine, l max : 0.5088 day -1 and K d : 0.132 day -1 .…”

A comprehensive overview of elements in bioremediation

“…Pyridine and pyridine based products are of major concern as environmental pollutants due to their recalcitrant, persistent, toxic and teratogenic nature. Lodha et al (2008) have studied the biodegradation of pyridine by an isolated consortium/strain under aerobic condition. Batch experiment results revealed that at lower initial pyridine concentrations (1-20 mg/l), almost complete degradation was observed whereas at higher concentration (30-50 mg/l), the degradation efficiency was dropped significantly.…”

Enhanced pyridine biodegradation under anoxic condition: The key role of nitrate as the electron acceptor

“…Most of these studies were concentrated on the isolation and characterization of pyridine-degrading microorganisms [10,11], bioaugmentation of the bioreactors by adding pyridine-degrading microorganisms [11][12][13], immobilization of the microorganisms capable of degrading pyridine [14,15], and so on. However, considering the nauseating odour at aerobic condition, pyridine degradation under anaerobic or anoxic condition has attracted increasing interest in recent years [16].…”