Bioinspired gold coated phage nanosomes for anti-microbial and anti-cancer theranostics

Yadav, Dokkari Nagalaxmi; Sankaranarayanan, Sri Amruthaa; Thanekar, Ajinkya Madhukar; Rengan, Aravind Kumar

doi:10.1016/j.mtnano.2023.100348

Cited by 11 publications

(12 citation statements)

References 36 publications

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“…The spot assay was conducted as per the established protocol. , Plates with the spots were placed in an incubator at 37 °C for 24 h. After the incubation period, the alteration in the spot count was assessed.…”

Section: Methodsmentioning

confidence: 99%

Multifunctional Nanosystem for Dual Anti-Inflammatory and Antibacterial Photodynamic Therapy in Infectious Diabetic Wounds

Ali,

Buddhiraju,

Gubige

et al. 2024

ACS Infect. Dis.

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Infectious diabetic wounds present a substantial challenge, characterized by inflammation, infection, and delayed wound healing, leading to elevated morbidity and mortality rates. In this work, we developed a multifunctional lipid nanoemulsion containing quercetin, chlorine e6, and rosemary oil (QCRLNEs) for dual anti-inflammatory and antibacterial photodynamic therapy (APDT) for treating infectious diabetic wounds. The QCRLNEs exhibited spherical morphology with a size of 51 nm with enhanced encapsulation efficiency, skin permeation, and localized delivery at the infected wound site. QCRLNEs with NIR irradiation have shown excellent wound closure and antimicrobial properties in vitro, mitigating the nonselective cytotoxic behavior of PDT. Also, excellent biocompatibility and anti-inflammatory and wound healing responses were observed in zebrafish models. The infected wound healing properties in S. aureus-infected diabetic rat models indicated re-epithelization and collagen deposition with no signs of inflammation. This multifaceted approach using QCRLNEs with NIR irradiation holds great promise for effectively combating oxidative stress and bacterial infections commonly associated with infected diabetic wounds, facilitating enhanced wound healing and improved clinical outcomes.

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Section: Methodsmentioning

confidence: 99%

Multifunctional Nanosystem for Dual Anti-Inflammatory and Antibacterial Photodynamic Therapy in Infectious Diabetic Wounds

Ali,

Buddhiraju,

Gubige

et al. 2024

ACS Infect. Dis.

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“…The NIR dye (IR780, ICG, IR783, IR806, IR820, IR895, and IR775)-loaded liposomes were synthesized as per the previously reported method. 48 Briefly, Egg PC and NIR dye were taken in a 10:1 ratio in 1 mL of ethanol and added to Milli-Q water under stirring at 65 °C. After 20 min of stirring, the as-synthesized liposomes were sonicated and used for further characterization.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Additionally, modifying these phage nanosomes with a gold coating has demonstrated superior photothermal performance compared to standard gold nanosystems. 48 In this study, we explore the role of phages in enhancing the optical and photothermal properties of existing NIR dyes through a facile synthesis method. We have synthesized NIR dye-loaded liposomes and phage nanosomes, for which we have chosen four lipophilic dyes (IR820, IR775, IR895, and IR780), one amphiphilic dye (ICG), and two hydrophilic dyes (IR806 and IR783), and we were able to load all of the dye molecules into the liposomes and phage nanosomes successfully, irrespective of their solubility.…”

Section: ■ Introductionmentioning

confidence: 99%

Tailoring Phage Nanosomes for Enhanced Theranostic Properties of Near Infrared Dyes

Sankaranarayanan,

Yadav,

Yadav

et al. 2024

Langmuir

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Near-infrared (NIR) phototherapies offer noninvasive, cost-effective solutions for treating tumors and microbial infections. However, organic NIR dyes commonly used suffer from solubility and stability issues requiring frequent dosing. We address this challenge by exploring the bacteriophage-mediated enhancement of NIR dye properties. Upon encapsulation within phage nanosomes, IR780 and Indocyanine green (ICG), with similar optical properties but distinct water solubility and exhibit enhanced UV−vis absorbance and photothermal transduction efficacy compared to liposomes. Experimental characterization corroborated with all-atom molecular dynamics simulations imprints the nanoscale structure, solubility, dynamics, and binding of these NIR dye molecules to the membrane and protein molecules present in Phage capsid. These NIR dye-loaded phage nanosomes, coencapsulated with mitoxantrone, demonstrate enhanced anticancer activity, and when combined with amphotericin B, these dye molecules exhibit superior photothermal effects against fungal infections. Our findings present a simple and efficient approach for tuning the photothermal performance of existing NIR dyes through a rational design for enhanced therapeutic outcomes.

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“…The effect of IR 792 PC, IR NPs, and AC IR NPs on the mitochondrial membrane potential was assessed using JC1 staining as per the protocol mentioned previously [22]. Briefly, the cells, upon treatment with IR 792 perchlorate, AC NPs, IR NPs, and AC IR NPs, were irradiated using 750 nm laser for 10 min.…”

Section: Assessment Of Mitochondrial Health (Jc1 Stain)mentioning

confidence: 99%

PLGA nanoparticle loaded with antioxidants and photosensitizer for ROS shock mediated phototherapy of triple negative breast cancer

Buddhiraju,

Balaraman,

Dehariya

et al. 2023

Biomed. Mater.

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The potential use of antioxidants for photodynamic therapy (PDT) is investigated in this study. PDT causes Reactive oxygen species (ROS)-mediated cell death; on the contrary, antioxidants scavenge ROS. The use of a photosensitizer along with an antioxidant photosensitizer compensates for the loss of ROS due to the use of antioxidant, eventually leading to cell death. In this work, for PDT and photothermal therapy (PTT), we have combined the photosensitizer IR 792 Perchlorate dye with the antioxidants alpha-tocopherol (A) and p-coumaric acid (C) encapsulated in a polymeric nanocarrier (AC IR NPs). We have reported the synthesis of AC IR NPs using Poly Lactic-co-Glycolic Acid (PLGA) by nanoprecipitation method. The size of the polymeric nanoparticle were found to be 80.4 ± 15.6 nm, with a spherical morphology observed by SEM and TEM. The synthesized AC IR NPs demonstrated good biocompatibility in fibroblast cell lines (L929). Furthermore, the efficacy assessment of the as prepared nanosystem in vitro on breast cancer cell lines (4T1) revealed a significant cell death of nearly 80%. This could be attributed to the ROS generation leading to oxidative stress and inhibition of metastasis. This study provides an evidence that the combination of antioxidant drugs along with photosensitizers have the potential to be an effective therapy for treating breast cancer.

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Bioinspired gold coated phage nanosomes for anti-microbial and anti-cancer theranostics

Cited by 11 publications

References 36 publications

Multifunctional Nanosystem for Dual Anti-Inflammatory and Antibacterial Photodynamic Therapy in Infectious Diabetic Wounds

Multifunctional Nanosystem for Dual Anti-Inflammatory and Antibacterial Photodynamic Therapy in Infectious Diabetic Wounds

Tailoring Phage Nanosomes for Enhanced Theranostic Properties of Near Infrared Dyes

PLGA nanoparticle loaded with antioxidants and photosensitizer for ROS shock mediated phototherapy of triple negative breast cancer

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