PLGA nanoparticle loaded with antioxidants and photosensitizer for ROS shock mediated phototherapy of triple negative breast cancer

Abstract

The potential use of antioxidants for photodynamic therapy (PDT) is investigated in this study. PDT causes Reactive oxygen species (ROS)-mediated cell death; on the contrary, antioxidants scavenge ROS. The use of a photosensitizer along with an antioxidant photosensitizer compensates for the loss of ROS due to the use of antioxidant, eventually leading to cell death. In this work, for PDT and photothermal therapy (PTT), we have combined the photosensitizer IR 792 Perchlorate dye with the antioxidants alpha-tocopherol (A) and p-coumaric acid (C) encapsulated in a polymeric nanocarrier (AC IR NPs). We have reported the synthesis of AC IR NPs using Poly Lactic-co-Glycolic Acid (PLGA) by nanoprecipitation method. The size of the polymeric nanoparticle were found to be 80.4 ± 15.6 nm, with a spherical morphology observed by SEM and TEM. The synthesized AC IR NPs demonstrated good biocompatibility in fibroblast cell lines (L929). Furthermore, the efficacy assessment of the as prepared nanosystem in vitro on breast cancer cell lines (4T1) revealed a significant cell death of nearly 80%. This could be attributed to the ROS generation leading to oxidative stress and inhibition of metastasis. This study provides an evidence that the combination of antioxidant drugs along with photosensitizers have the potential to be an effective therapy for treating breast cancer.

Ursolic acid loaded tri-block copolymer nanoparticles based on triphenylphosphine for mitochondria-targeted cancer therapy

“…Currently, polymers, especially biodegradable polymers, play an indispensable role in drug delivery systems and have become an attractive research focus [18,19]. Due to its environmentally friendly and nontoxic degradable properties, polylactic acid (PLLA) is widely used in the human body and in many fields, such as biomedicine, environmental engineering, and food packaging, etc [20][21][22]. However, nanocarriers prepared with PLLA are highly susceptible to recognition and phagocytosis by the reticuloendothelial system because of their hydrophobic surface.…”