Bioinspired gelatin/bioceramic composites loaded with bone morphogenetic protein-2 (BMP-2) promote osteoporotic bone repair

Echave, Mari Carmen; Erezuma, Itsasne; Golafshan, Nasim; Castilho, Miguel; Kadumudi, Firoz Babu; Pimenta-Lopes, Carolina; Ventura, Francesc; Pujol, Anna; Jiménez, J.; Cámara, Juan Antonio; Hernáez‐Moya, Raquel; Iturriaga, Leire; Burgo, Laura Sáenz del; Iloro, Ibón; Azkargorta, Mikel; Elortza, Félix; Lakshminarayanan, Rajamani; Al‐Tel, Taleb H.; García-García, Patricia; Reyes, Ricardo; Delgado, Araceli; Évora, Carmen; Pedraz, José Luís; Dolatshahi-Pirouz, Alireza; Orive, Gorka

doi:10.1016/j.msec.2021.112539

Cited by 17 publications

(15 citation statements)

References 60 publications

(64 reference statements)

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“…Widely described physical methods rely on using UV light or microwave energy to rearrange gelatin's amino acid sequences, but this approach is more likely to lack efficacy, as it is more difficult to have good control of the crosslink density. There have also been attempts with chemical agents that range from synthetic polymers (glutaraldehyde) to natural enzymes (transglutaminase) [18,[30][31][32]. Synthetic polymers have shown controlled synthesis, but the byproducts may result in cytotoxicity, whereas enzymes may not generate chemical waste materials, as they are natural and bond gelatin fibers [30,[32][33][34][35].…”

Section: Gelatin As a Systemmentioning

confidence: 99%

“…However, its porosity is always desirable for regenerative medicine approaches [53]. The freeze-drying technique enables modification of the pore diameter, the result being a reorganized permeable structure capable of improving cell adhesion and the regenerative capacity of gelatin as a biomaterial [18,30]. Therefore, the freeze-drying process could help to produce highly porous and mechanically stable gelatin-based structures (Figure 3).…”

Section: Freeze-drying Techniquementioning

confidence: 99%

“…Furthermore, this technique has become widespread, as it has also proven to be advantageous for cell protection as well as for the release of biologically active agents [17,36,54,55]. The freeze-drying technique enabled gelatin to form a porous hydrogel, which helped to attract cells responsible for osteogenesis [18,30]. In other tissues, such as neural ones, the challenge lies in finding the proper alignment of pores.…”

Section: Freeze-drying Techniquementioning

confidence: 99%

“…Thus, one of the most widely used biomaterials in the area is gelatin. Specifically, the fact that it originates from collagen makes this material suitable for orthopedics and it has already been widely employed in a myriad of systems such as drug delivery systems, hydrogels, scaffolds or films for wound dressing [16][17][18][19]. Additionally, the Food and Drug Administration (FDA) has approved a blend of demineralized bone matrix and gelatin (DBX Strips) for bone tissue engineering, together with absorbable gelatin sponges (Surgiflo ® , Ferrosan Medical Devices A/S, Søborg, Denmark, Cutanplast ® , Mascia Brunelli S.p.a., Milano, Italy) to maintain hemostasis in multiple surgeries [20,21].…”

Section: Introductionmentioning

confidence: 99%

“…Copyright 2022, American Chemical Society. Recently, Echave et al developed an enzymatically cross-linked gelatin-based scaffold.The freeze-drying technique enabled gelatin to form a porous hydrogel, which helped to attract cells responsible for osteogenesis[18,30]. In other tissues, such as neural ones, the challenge lies in finding the proper alignment of pores.…”

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Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances in the areas of technology and biomaterials have facilitated the use of engineered constructs for medical issues. This review discusses on-going concerns and the latest developments in a widely employed biomaterial in the field of tissue engineering: gelatin. Emerging techniques including 3D bioprinting and gelatin functionalization have demonstrated better mimicking of native tissue by reinforcing gelatin-based systems, among others. This breakthrough facilitates, on the one hand, the manufacturing process when it comes to practicality and cost-effectiveness, which plays a key role in the transition towards clinical application. On the other hand, it can be concluded that gelatin could be considered as one of the promising biomaterials in future trends, in which the focus might be on the detection and diagnosis of diseases rather than treatment.

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Section: Gelatin As a Systemmentioning

confidence: 99%

Section: Freeze-drying Techniquementioning

confidence: 99%

Section: Freeze-drying Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Progress in Gelatin as Biomaterial for Tissue Engineering

et al. 2022

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Structural and Functional Adaptive Artificial Bone: Materials, Fabrications, and Properties

Feng

Zhao

Tang

et al. 2023

Adv Funct Materials

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It is an urgent need that defect repair can develop from simple device fixation to living tissue reconstruction, from short life function replacement to permanent regeneration repair. At present, bone transplantation has become the second largest transplantation surgery after blood transfusion, and artificial bone transplantation generates great hope for the repair and treatment of bone defect. In order to repair bone defect, artificial bone must have good biological properties and sufficient mechanical properties, and it should also have the shape matching to bone defect site and the connected porous structure. For structures and properties requirements of artificial bone, in this review three major challenges faced by artificial bone transplantation are systemtically analyzed and current methods and strategies to address these issues are discussed: 1) the need for developing a type of bone scaffold material with both biological and mechanical properties, 2) the need for realizing the controllable fabrication of individual shape and multistage pore structure of bone scaffold, 3) the need for realizing the transformation from man‐made structure to biological structure. Besides, it summarizes the advantages and disadvantages of these methods and discusses the potential future directions of structural and functional adaptive artificial bone for bone defect regeneration.

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3D Bioprinted Hydroxyapatite or Graphene Oxide Containing Nanocellulose‐Based Scaffolds for Bone Regeneration

Lafuente‐Merchan

Ruiz‐Alonso

García‐Villén

et al. 2022

Macromolecular Bioscience

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Bone tissue is usually damaged after big traumas, tumors, and increasing aging-related diseases such as osteoporosis and osteoarthritis. Current treatments are based on implanting grafts, which are shown to have several inconveniences. In this regard, tissue engineering through the 3D bioprinting technique has arisen to manufacture structures that would be a feasible therapeutic option for bone regenerative medicine. In this study, nanocellulose-alginate (NC-Alg)-based bioink is improved by adding two different inorganic components such as hydroxyapatite (HAP) and graphene oxide (GO). First, ink rheological properties and biocompatibility are evaluated as well as the influence of the sterilization process on them. Then, scaffolds are characterized. Finally, biological studies of embedded murine D1 mesenchymal stem cells engineered to secrete erythropoietin are performed. Results show that the addition of both HAP and GO prevents NC-Alg ink from viscosity lost in the sterilization process. However, GO is reduced due to short cycle autoclave sterilization, making it incompatible with this ink. In addition, HAP and GO have different influences on scaffold architecture and surface as well as in swelling capacity. Scaffolds mechanics, as well as cell viability and functionality, are promoted by both elements addition. Additionally, GO demonstrates an enhanced bone differentiation capacity.

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Bioinspired gelatin/bioceramic composites loaded with bone morphogenetic protein-2 (BMP-2) promote osteoporotic bone repair

Cited by 17 publications

References 60 publications

Progress in Gelatin as Biomaterial for Tissue Engineering

Progress in Gelatin as Biomaterial for Tissue Engineering

Structural and Functional Adaptive Artificial Bone: Materials, Fabrications, and Properties

3D Bioprinted Hydroxyapatite or Graphene Oxide Containing Nanocellulose‐Based Scaffolds for Bone Regeneration

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