Bioink: a 3D-bioprinting tool for anticancer drug discovery and cancer management

Tiwari, Arpita; Thorat, Nanasaheb D.; Pricl, Sabrina; Patil, Rahul; Rohiwal, Sonali S.; Townley, Helen E.

doi:10.1016/j.drudis.2021.03.010

Cited by 32 publications

(32 citation statements)

References 95 publications

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“…The use of light absorbers to prevent unwanted polymerization by scattered light allows high-resolution stereolithography of multi-vascular networks as demonstrated by the development of a vascularized alveolar model [ 4 ]. These different methods are beyond the scope of this mini-review and discussed in more detail in recent review articles [ 1 , 30 , 31 ].…”

Section: Bioprinting Methods and Procedures To Build Complex Tissue Equivalents For Tissue Regeneration And Disease Modellingmentioning

confidence: 99%

3D bioprinting: novel approaches for engineering complex human tissue equivalents and drug testing

Hagenbuchner

Nothdurfter

Ausserlechner

2021

Essays in Biochemistry

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Conventional approaches in drug development involve testing on 2D-cultured mammalian cells, followed by experiments in rodents. Although this is the common strategy, it has significant drawbacks: in 2D cell culture with human cells, the cultivation at normoxic conditions on a plastic or glass surface is an artificial situation that significantly changes energy metabolism, shape and intracellular signaling, which in turn directly affects drug response. On the other hand, rodents as the most frequently used animal models have evolutionarily separated from primates about 100 million years ago, with significant differences in physiology, which frequently leads to results not reproducible in humans. As an alternative, spheroid technology and micro-organoids have evolved in the last decade to provide 3D context for cells similar to native tissue. However, organoids used for drug testing are usually just in the 50–100 micrometers range and thereby too small to mimic micro-environmental tissue conditions such as limited nutrient and oxygen availability. An attractive alternative offers 3D bioprinting as this allows fabrication of human tissue equivalents from scratch with hollow structures for perfusion and strict spatiotemporal control over the deposition of cells and extracellular matrix proteins. Thereby, tissue surrogates with defined geometry are fabricated that offer unique opportunities in exploring cellular cross-talk, mechanobiology and morphogenesis. These tissue-equivalents are also very attractive tools in drug testing, as bioprinting enables standardized production, parallelization, and application-tailored design of human tissue, of human disease models and patient-specific tissue avatars. This review, therefore, summarizes recent advances in 3D bioprinting technology and its application for drug screening.

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Section: Bioprinting Methods and Procedures To Build Complex Tissue Equivalents For Tissue Regeneration And Disease Modellingmentioning

confidence: 99%

3D bioprinting: novel approaches for engineering complex human tissue equivalents and drug testing

Hagenbuchner

Nothdurfter

Ausserlechner

2021

Essays in Biochemistry

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“…Moreover, PBB is typically equipped with a set of UV lights, a movement table or bioprinting probe, a material tank, and a computer [133,134]. PBB technology based on ultraviolet (UV) light rapid curing technology can achieve complex geometry and small-size model manufacturing, which makes up for the shortage of extrusion 3D bioprinting technology to a certain extent [135,136]. In the field of vascularization, the most common photoinitiators are Irgacure 2959 (maximum effective wavelength 275 nm) and phenyl-2,4, 6-trimethylbenzoyl phosphate (LAP, maximum effective wavelength 375 nm), which are less cytotoxic [132].…”

Section: Photopolymerization-based Bioprintingmentioning

confidence: 99%

The Biofabrication of Diseased Artery In Vitro Models

et al. 2022

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As the leading causes of global death, cardiovascular diseases are generally initiated by artery-related disorders such as atherosclerosis, thrombosis, and aneurysm. Although clinical treatments have been developed to rescue patients suffering from artery-related disorders, the underlying pathologies of these arterial abnormalities are not fully understood. Biofabrication techniques pave the way to constructing diseased artery in vitro models using human vascular cells, biomaterials, and biomolecules, which are capable of recapitulating arterial pathophysiology with superior performance compared with conventional planar cell culture and experimental animal models. This review discusses the critical elements in the arterial microenvironment which are important considerations for recreating biomimetic human arteries with the desired disorders in vitro. Afterward, conventionally biofabricated platforms for the investigation of arterial diseases are summarized, along with their merits and shortcomings, followed by a comprehensive review of advanced biofabrication techniques and the progress of their applications in establishing diseased artery models.

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“…Based on the printing method, bio-ink should possess tunable, mechanical strength and viscosity to support the growth, viability, proliferation, and functionalization of cells. Bio-inks are categorized into: (1) Protein/peptide polymer-based bio-ink (2) Carbohydrate-based bio-ink, (3) Extracellular-based bio-ink, (4) Synthetic polymer bio-ink, (5) Cell aggregate bio-ink, and (6) Composite bio-ink, which can be used for regenerative medicine, drug delivery, and tissue engineering [59]. In this section, we have discussed the most widely used bio-inks for fabricating 3D constructs.…”

Section: Bio-inkmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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Three-dimensional (3D) printing is a technique where the products are printed layer-by-layer via a series of cross-sectional slices with the exact deposition of different cell types and biomaterials based on computer-aided design software. Three-dimensional printing can be divided into several approaches, such as extrusion-based printing, laser-induced forward transfer-based printing systems, and so on. Bio-ink is a crucial tool necessary for the fabrication of the 3D construct of living tissue in order to mimic the native tissue/cells using 3D printing technology. The formation of 3D software helps in the development of novel drug delivery systems with drug screening potential, as well as 3D constructs of tumor models. Additionally, several complex structures of inner tissues like stroma and channels of different sizes are printed through 3D printing techniques. Three-dimensional printing technology could also be used to develop therapy training simulators for educational purposes so that learners can practice complex surgical procedures. The fabrication of implantable medical devices using 3D printing technology with less risk of infections is receiving increased attention recently. A Cancer-on-a-chip is a microfluidic device that recreates tumor physiology and allows for a continuous supply of nutrients or therapeutic compounds. In this review, based on the recent literature, we have discussed various printing methods for 3D printing and types of bio-inks, and provided information on how 3D printing plays a crucial role in cancer management.

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Bioink: a 3D-bioprinting tool for anticancer drug discovery and cancer management

Cited by 32 publications

References 95 publications

3D bioprinting: novel approaches for engineering complex human tissue equivalents and drug testing

3D bioprinting: novel approaches for engineering complex human tissue equivalents and drug testing

The Biofabrication of Diseased Artery In Vitro Models

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

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