Biohydrogen and Biomethane (Biogas) Production in the Consecutive Stages of Anaerobic Digestion of Molasses

Detman, Anna; Chojnacka, Aleksandra; Błaszczyk, Mieczysław; Kaźmierczak, Wiktor; Piotrowski, Jan; Sikora, Anna

doi:10.15244/pjoes/68149

Cited by 33 publications

(30 citation statements)

References 20 publications

(27 reference statements)

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“…However, compared to this study, the previous media contained a higher concentration of a signi cant consumption of lactate (or its low concentration) among the non-gaseous products despite the presence of lactic acid bacteria in the microbial communities. It is in accordance with the previous studies where the e uents from hydrogen producing bioreactors and the microbial communities were examined [46,47,50]. It can also explain a lack or a weak correlation between Lactobacillus and lactate in Experiments ML, MLA and SLA.…”

Section: Discussionsupporting

confidence: 91%

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Dynamics of Dark Fermentation Microbial Communities in the Light of Lactate and Butyrate Production

Detman

Laubitz

Chojnacka

et al. 2020

Preprint

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Background: This study focuses on the processes occurring during acidogenic step of anaerobic digestion, especially resulting from nutritional interactions between dark fermentation (DF) bacteria and lactic acid bacteria (LAB). Previously, we have confirmed that DF microbial communities fed on molasses are able to convert lactate and acetate to butyrate in batch experiments. The aims of the study were: (i) to recognize biodiversity of DF microbial communities able and unable to convert lactate and acetate to butyrate and (ii) to define the conditions for the transformation in static batch experiments.Results: Sucrose stimulated bacterial growth, especially LAB. In the samples where the microbial communities fermented media containing carbohydrates the two main tendencies were observed: (i) a low pH (pH≤4), lactate and ethanol as the main fermentation products, microbial communities dominated with Lactobacillus, Bifidobacterium, Leuconostoc and Fructobacillus was characterised by a low biodiversity; (ii) pH in the range 5.0-6.0, butyrate dominated among the fermentation products, the microbial communities composed mailny of Clostridium (especially Clostridium sensu stricto 12), Lactobacillus, Bifidobacterium and Prevotella. The biodiversity increased with the ability to convert acetate and lactate to butyrate. The microbial communities processing exclusively lactate and acetate showed the highest biodiversity and was dominated by Clostridium (especially Clostridium sensu stricto 12). LAB were reduced, other genera such as Terrisporobacter, Lachnoclostridium, Paraclostridium or Sutterella were found. Butyrate was the main metabolite and pH was 7. WGS analysis of the selected butyrate-producing microbial communities independently on the substrate, revealed C. tyrobutyricum as a dominant Clostridium species. Conclusions: The batch tests revealed dynamics of metabolic activity and composition of DF microbial communities dependent on fermentation conditions. The results expand our knowledge on lactate to butyrate conversion by DF microbial communities. The relevant factor for conversion of lactate and acetate to butyrate in the presence of carbohydrates is pH in the range 5-6 and the balance between LAB (especially Lactobacillus), lactate and acetate producers (Bifidobacterium) and butyrate producers (mainly Clostridium) as well Prevotella. The pH below 4 and ethanol concentration might be the signalling factors responsible for metabolic shift of the dark fermentation microbial communities towards lactate fermentation.

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Section: Discussionsupporting

confidence: 91%

“…The media contained molasses as a source of sucrose or pure sucrose with addition of lactate and acetate, or exclusively a mixture of lactate and acetate. Molasses has been used in our studies for hydrogen and methane production in a two-stage process [46][47][48]. Sucrose is an attractive substrate for glycolytic fermentations.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Dark Fermentation Microbial Communities in the Light of Lactate and Butyrate Production

Detman

Laubitz

Chojnacka

et al. 2020

Preprint

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“…2b). Our results are consistent with De Vrieze et al [14] and Detman et al [8] regarding the COD removal rate. However, De Vrieze et al [14] reported a rapid decrease in COD removal rate at OLR of 4.1 g COD L − 1 .…”

Section: Resultssupporting

confidence: 93%

“…An important organic agro-industrial byproduct, molasses is a highly dense material produced through the crystallization of sugarcane [7]. It is also considered a major raw material for biogas production due to its high organic fraction content [1,8]. However, despite the increasing interest molasses receives and despite its high organic fraction content, its anaerobic digestion is limited due to its high COD, its high ion concentration, and its melanoidin content; therefore, molasses should be diluted strongly before it can be used in any biological process [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

Khan

Jiang

et al. 2020

Preprint

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Background Molasses is a highly dense and refined byproduct produced in the sugarcane industry, and it contains high amounts of degradable compounds. These compounds can potentially be converted into renewable products biologically. However, the involved biological process is negatively influenced by the high chemical oxygen demand (COD) of molasses and its high ion concentration, although this problem is commonly addressed by dilutions. Results The co-digestion of molasses with rice alcohol waste water (RAW) was compared with its mono-digestion at an increasing organic loading rate (OLR). Both processes were assessed by detecting the COD removal rate, the methane contents of biogas, and the structure and composition of microbial communities at different stages. Results showed that the co-digestion is stable up to a maximum OLR of 16 g COD L− 1d− 1. By contrast, after the acclimatization phase, the mono-digestion process was upset twice, which occurred at a maximum OLR of 9 and 10 g COD L− 1d− 1. The co-digestion procgess demonstrated consistency in terms of COD removal rates (86.36% ± 0.99–90.72% ± 0.63%) and methane contents (58.10% ± 1.12–64.47% ± 0.59%) compared with the mono-digestion process. Microbial community analysis showed that the relative abundance of bacterial and archaeal communities differs between the processes at different stages. However, in both processes, Propionibacteriaceae was the most abundant family in the bacterial communities, whereas Methanosaetaceae was abundant in the archaeal communities. Conclusion Rice alcohol wastewater could be a good co-substrate for anaerobic digestion of molasses. Integrate molasses into progressive biogas production at high OLR.

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“…Other study used the sugar beet molasses to hydrogen and methane production as a result of two-stage anaerobic digestion [29].…”

Section: Introductionmentioning

confidence: 99%

Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses

Jame¹,

Lakatoš

Hassan³

et al. 2017

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The study had aimed to characterize the production of hydrogen gases by anaerobic bacteria. One isolate was found in sheep ruminal fluid and four isolates were obtained from the activated sludge. These isolates were identified by microscopic methods and by rRNA sequences. One ruminal bacterium was identified as Escherichia coli, and it was found that these isolates from activated sludge were related to Clostridium botulinum, C. perfringens and C. difficile. One strain could not be assigned to any species but was similar to C. botulinum. Growth and production of the metabolic gases with molasses as sole carbon source were measured during the anaerobic cultivation by Micro-Oxymax (Columbus Instruments, Columbus, OH, U.S.A.) gas analyzer. One of the most available saccharidic waste products is molasses. The growth on molasses as carbon source was done to test the production of H 2 . It was found that all tested Clostridium isolates (AK 1-4, AK 1-5, AK 1-9 and AK 1-12) and E. coli isolate (No 2-24) had utilized molasses as carbon source monitored by production of CO 2 gas. All these strains produced H 2 gas, and CO gas in concentration range 102 µmol L -1 , and H 2 S gas in concentrations lower by one order of magnitude. Kinetics of evolution of these gases was different suggesting that they are produced by independent processes. Results show that metabolic gases are produced mainly in the exponential phase of growth.

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Biohydrogen and Biomethane (Biogas) Production in the Consecutive Stages of Anaerobic Digestion of Molasses

Cited by 33 publications

References 20 publications

Dynamics of Dark Fermentation Microbial Communities in the Light of Lactate and Butyrate Production

Dynamics of Dark Fermentation Microbial Communities in the Light of Lactate and Butyrate Production

Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses

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