Biogenic Silver and Zero-Valent Iron Nanoparticles by Feijoa: Biosynthesis, Characterization, Cytotoxic, Antibacterial and Antioxidant Activities

Abstract

Background: and Purpose: Green nanotechnology is an interesting method for the synthesis of functional nanoparticles. Because of their wide application, they have set up great attention in recent years. Objective: The present research examines the green synthesis of Ag and zero-valent iron nanoparticles (AgNPs, ZVINPs) by Feijoa sellowiana fruit extract. In this synthesis, no stabilizers or surfactants were applied. Methods: Eco-friendly synthesis of Iron and biogenic synthesis of Ag nanoparticles were accomplished by controlling critical parameters such as concentration, incubation period and temperature. Scanning Electron Microscopy (SEM), Transmission Electron Microscope (TEM), Energy-Dispersive X-ray Spectroscopy (EDS), Fourier-Transform Infrared (FT-IR) spectroscopy, X-ray Diffraction analysis (XRD), Dynamic Light Scattering (DLS) and UV-Vis were applied to characterize NPs. The cytotoxicity of NPs were investigated in two cell lines, MCF-7 (breast cancer) and AGS (human gastric carcinoma). A high-performance liquid chromatography (HPLC) analysis was also performed for characteization phenolic acids in the extract. Results: Both NPs displayed powerful anticancer activities against two tumor cell lines with little effect on BEAS-2B normal cells. Synthesized AgNPs and ZVINPs inhibited the growth of all selected bacteria. Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumonia, Staphylococcus aureus, Enterococcus faecalis, Acinetobacter baumannii and Escherichia coli have been studied in two stages. We initially examined the ATCCs followed by clinical strain isolation. Based on the results from resistant strains, we showed that nanoparticles were superior to conventional antibiotics. DPPH (diphenyl-1-picrylhydrazyl) free radical scavenging assay and iron chelating activity were used for determination of antioxidant properties. Results showed a highly antioxidant activity for scavenging of free radicals for ZVINPs and powerful iron chelating activity for AgNPs. Based on the HPLC data, catechin was the major phenolic compound in the extract. Conclusion: Our synthesized nanoparticles displayed potent cytotoxic, antibacterial and antioxidant activities.

Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties

“…The effect of incubation periods: Figure 2a clearly demonstrates that the absorbance of the mixture increased as the contact time increased from 30 min to 240 min, due to the evolution of the reduction in silver ions and the increase in the number of silver nanoparticles [54]. The UV-Vis spectra exhibited strong SPR absorbance after 240 min due to the stabilization of nanoparticles because of the stabilization agent present in the plant extract [55]. Then, the rate of reaction of the lemon zest mediated silver nanoparticles is 4 h, which is faster compared with the biosynthesis of AgNPs using Citrus tangerina, Citrus sinensis, and Citrus limon peel extracts [56].…”

Protective effect of Feijoa sellowianan fruit on testicular toxicity‐induced by cadmium chloride

“…Conventional HPLC was used using a C18 column to isolate phenolic acids. The extract contained 5 phenolic compounds such as catechins, gallic acid, caffeic acid, routine and p-coumaric acid (188.5, 18.5, 2.3, 8.15 and 4.7 g/ g, respectively;Hashemi et al, 2020).…”

Emerging Trends in Advanced Translational Applications of Silver Nanoparticles: A Progressing Dawn of Nanotechnology

“…AgNPs have been widely researched for their antibacterial properties against bacteria such as P. aeruginosa , P. vulgaris , E. coli , S. aureus , etc. [ 29 ]. A recent investigation by Verma et al reported the inverse cytotoxicity and enhanced antibacterial activity of the green-synthesized AgNPs [ 30 ].…”