Abstract:Biogenic coal bed methane (CBM) has gained considerable interest because of its potential to be regenerated. The research reported in this thesis evaluated the potential to develop Indonesian coals as CH 4 bioreactors using culture enrichment, molecular phylogenetic and isotopic composition analysis methods. The results increase the current understanding on microbial methanogenesis in coal, with a specific focus on Indonesian coals.Indonesian coals range in rank from lignite to bituminous, with anthracites occ… Show more
“…Based on MG-Rast result, the genus found in coal waste dominated by Clostridium 51.28%, followed with Bacillus 35.29%, Pyramidobacter 6.13%, Desulfovibrio 2.04%, Planctomyces 1.38%, and Syntrophomonas 0.56% (Figure 1). This was consistent with (Susilawati 2015), who stated that bacterial species from the Clostridium genus were geopolymer hydrolyzers in biogenic Coal Bed Methane (CBM) reservoirs. This showed that after applying the treatment and growing bacteria in waste, it still showed biogenic coal.…”
Increasing energy demand is not alongside the availability of limited fossil fuels. Alternative and renewable energy sources are not only an option to overcome energy problems but also essential to minimize global warming. Another critical and promising renewable energy source is biomass-derived from livestock feces. Beef cattle feces contain a microorganism consortium that can be used as a starter with coal media to form biogas. Indonesia recently developed coal waste processing into renewable energy, such as biogas. This study aimed to overview the ecological diversity of microbial consortium of beef cattle feces, lignite coal waste, and a combination of livestock and lignite coal waste under mesophilic conditions. This research is an explorative method, the data obtained were analyzed descriptively. The process of formation was carried out anaerobically on a bottle containing the rumen fluid medium. The fermentation process lasted 42 days at 39℃ of temperature. After that, the sample was electrophoresis, followed by next-generation sequencing (NGS) method. NGS data is processed with the MG-Rast website. This study demonstrates the ecological diversity of microbial consortium of beef cattle, lignite coal waste, and a combined consortium. The results showed ecological diversity in the form of taxonomy dominated by bacteria, eukaryotes, and archaea.
“…Based on MG-Rast result, the genus found in coal waste dominated by Clostridium 51.28%, followed with Bacillus 35.29%, Pyramidobacter 6.13%, Desulfovibrio 2.04%, Planctomyces 1.38%, and Syntrophomonas 0.56% (Figure 1). This was consistent with (Susilawati 2015), who stated that bacterial species from the Clostridium genus were geopolymer hydrolyzers in biogenic Coal Bed Methane (CBM) reservoirs. This showed that after applying the treatment and growing bacteria in waste, it still showed biogenic coal.…”
Increasing energy demand is not alongside the availability of limited fossil fuels. Alternative and renewable energy sources are not only an option to overcome energy problems but also essential to minimize global warming. Another critical and promising renewable energy source is biomass-derived from livestock feces. Beef cattle feces contain a microorganism consortium that can be used as a starter with coal media to form biogas. Indonesia recently developed coal waste processing into renewable energy, such as biogas. This study aimed to overview the ecological diversity of microbial consortium of beef cattle feces, lignite coal waste, and a combination of livestock and lignite coal waste under mesophilic conditions. This research is an explorative method, the data obtained were analyzed descriptively. The process of formation was carried out anaerobically on a bottle containing the rumen fluid medium. The fermentation process lasted 42 days at 39℃ of temperature. After that, the sample was electrophoresis, followed by next-generation sequencing (NGS) method. NGS data is processed with the MG-Rast website. This study demonstrates the ecological diversity of microbial consortium of beef cattle, lignite coal waste, and a combined consortium. The results showed ecological diversity in the form of taxonomy dominated by bacteria, eukaryotes, and archaea.
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