Biogenesis of silver nanoparticles using leaf extract of Indigofera hirsuta L. and their potential biomedical applications (3-in-1 system)

Abstract

The present study reports the biosynthesis of silver nanoparticles (IH-AgNPs) using aqueous leaf extract of Indigofera hisruta L. The biosynthesized IH-AgNPs were found to be FCC crystals, 5-10 nm in size, spherical in shape and stable. The biosynthesized IH-AgNPs showed dose-dependant cytotoxicity against prostate cancer (PC3) (IC = 68.5 μg/mL), colon cancer (COLO205) (IC = 85.2 μg/mL), and mouse melanoma (B16F10) (IC = 80.9 μg/mL). IH-AgNPs were found to be nontoxic towards normal CHO (Chinese hamster ovary) cells. The biosynthesized IH-AgNPs showed effective in vitro antioxidant activity against DPPH (IC = 63.43 μg/mL) and HO (IC = 89.93 μg/mL) radicals. IH-AgNPs exhibited effective antibacterial activity against both Gram+ve and Gram-ve bacteria. MIC values of IH-AgNPs against S. aureus, B. subtilis, P. aeruginosa and E. coli were found to be 7.8 μg/mL, 3.9 μg/mL, 15.6 μg/mL and 15.6 μg/mL respectively. IH-AgNPs also showed inhibitory activity against fungal pathogens including C. albicans, C. nonalbicans and C. tropicalis. Considering the results together, we demonstrate that IH-AgNPs exhibits three different bioactivities (3-in-1 system) and they could be employed as future antimicrobial, antioxidant and anticancer agents/drug delivery vehicles in the field of biomedicine.

Eco-friendly synthesis and evaluation of biological activity of silver nanoparticles from leaf extract of Indigofera barberi Gamble: an endemic plant of Seshachalam Biosphere Reserve

“…Crystalline nature is one of the important aspects of AgNPs for their effective biological functions. The results of XRD are in consistence with previous reports [11,12].…”

“…The results are in consistence with many previous reports. I. hirsuta leaf synthesized AgNPs showed effective free radical scavenging activity against both DPPH and H 2 O 2 radicals [11]. Cibotium barometz root mediated synthesized AgNPs showed effective free radical scavenging activity against DPPH radicals [34], Bergenia ciliate [51], Chenopodium murale [52] and Rhododendron dauricum [15] extract synthesized AgNPs showed effective antioxidant activity against DPPH radicals.…”

“…Silver nanoparticles (AgNPs) have been reported to play a key role in the diagnosis, drug delivery, bioimaging [8], treatment of neurodegenerative (Alzheimer's and Parkinson's), autoimmune (rheumatoid arthritis), cardiovascular and diabetic diseases [8][9][10]. AgNPs have been found to possess antimicrobial, antioxidant, and anticancer activities [11][12][13]. The antimicrobial activity of the AgNPs is an important application in the biomedicine and biotherapeutics to prepare medicines, injections, surgical vials, implants and various diagnostic kits [14].…”

“…To our knowledge, there have been two reports available on silver nanoparticle synthesis from Indigofera species such as Indigofera tinctoria [42] and Indigofera hirsuta [11]. However, no reports on silver nanoparticle synthesis using I. barberi aqueous leaf extract as a reducing agent.…”

Fabrication of silver nanoparticles using Arnebia hispidissima (Lehm.) A. DC. root extract and unravelling their potential biomedical applications

“…Among the metal nanoparticles, silver nanoparticles (AgNPs) are highly explored as they are easy to synthesize, show tunable photophysical attributes, good conductivity, chemical stability, catalytic and antimicrobial activity. Silver nanoparticles possess various biological applications including bioimaging, biosensors, gene delivery, catalysis, antimicrobial, antioxidant and anticancer agents [2]. Medicinal plants are known to be of paramount importance attributed to the plethora of phytoconstituents contributing to their great potential for the synthesis of nanoparticles.…”