Eco-friendly synthesis and evaluation of biological activity of silver nanoparticles from leaf extract of Indigofera barberi Gamble: an endemic plant of Seshachalam Biosphere Reserve

Abstract

The present study reports the simple, rapid and ecofriendly synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Indigofera barberi. The aqueous leaf extract of I. barberi reduces the silver ions (Ag+) into AgNPs (silver nanoparticles synthesized from I. barberi leaf extract). Fourier transform infrared spectroscopy analysis showed the broad peaks at 1054, 1371, 1589, 2355, 2933, and 3257 cm −1 which indicated the participation of polyhydroxy compounds and proteins respectively in the biosynthesis and stabilization of AgNPs. TEM analysis showed that the AgNPs synthesized in this study were spherical in shape and having a dimension of 5-20 nm. XRD analysis of AgNPs confirmed the crystalline nature with face centered cubic lattice. Particle size analysis showed that AgNPs were 2-10 nm in size with average hydrodynamic radius of 3.4 nm. Zeta potential value of the AgNPs was found to be − 13.3 mV. AgNPs showed effective antimicrobial activity against both Gram−ve and Gram+ve bacteria. AgNPs showed effective antioxidant activity by quenching DPPH and H 2 O 2 radicals with IC-50 values of 67.37 and 72.04 µg/mL, respectively.

Synthesis of silver nanoparticles using extract of Ocimum kilimandscharicum and its antimicrobial activity against plant pathogens

“…Several studies have been reported on plantassisted reduction of metal nanoparticles. The synthesis of silver nanoparticles using a wide range of plant extracts like Aloe vera [32], Myrmecodia pendans [33], Desmodium gangeticum [34], Indigofera barberi [35], Euphorbia helioscopia [36], Artimisia vulgaris [37], Taraxacum officinale [38], Convolvulus arvensis [39] is reported. The main phytochemicals responsible for biosynthesis of silver nanoparticles are alkaloids, terpenoids, phenolic compounds, enzymes, coenzymes, protein and sugars [17].…”

Novel green synthesis of silver nanoparticles using clammy cherry (Cordia obliqua Willd) fruit extract and investigation on its catalytic and antimicrobial properties

“…Recently, the biosynthesis of metal nanoparticles (MNPs) have been proposed as a cost-effective and eco-friendly substitute to chemical methods. Many studies have been demonstrated the biosynthesis of AgNPs mediated by the microorganism or by phytochemicals or enzymatic synthesis [11,18,19,[29][30][31][32][33]. Among the biological methods, photosynthesis which involves reduction by phytochemicals derived from plants seems to be the superior choice.…”