Biogenesis and Characterization of Proficient Silver Nanoparticles Employing Marine Procured Fungi Hamigera Pallida and Assessment of their Antioxidative, Antimicrobial and Anticancer Potency on Human Breast Cancer (MCF-7) Cell Line

Abstract

Objectives To assess the anticancer potential of biosynthesized silver nanoparticles using marine derived fungi Hamigera pallida with their antibacterial and antioxidant activities. Results The biosynthesis of silver nanoparticles (AgNPs) was assessed by the change in color from bright yellow to dark brown. UV-Visible spectroscopy revealed its stability at 429 nm; ATR-FTIR spectroscopy revealed the functional group responsible for its production; X-Ray Diffraction revealed its crystalline FCC structure resembling the peaks in the XRD pattern, corresponding to (110), (111), (200), and (311) planes; TEM imaging revealed its spherical morphology with an average particle size of 5.85 ± 0.84 nm ranging from 3.69 to 16.11 nm and Tauc's plot analysis revealed a band gap energy of 2.22 eV, revealing aptitude of AgNPs as a semiconductors. The subsequent characterization results revealed the effective synthesis of silver nanoparticles. The biosynthesized AgNPs were found to have significant antimicrobial effect against three Gram-positive and three Gram-negative bacteria. They also demonstrated higher antioxidative potential by demonstrating strong radical scavenging activity against DPPH (2, 2-diphenyl-1-picrylhydrazyl). AgNPs showed highest anticancer activity (62.69 ± 1.73%) against human breast cancer (MCF-7) cell line at 100 µg/mL with the IC50 value of 66.07 ± 2.17 µg/mL. Conclusions This study revealed the prospect for further utilization of AgNPs by Cell free filtrate (CFF) of Hamigera pallida as an antibacterial, antioxidative and anticancer agents.