Biofunctionalized chondrogenic shape-memory ternary scaffolds for efficient cell-free cartilage regeneration

Xuan, Huixia; Hu, Haoran; Geng, Congying; Song, Jianchun; Shen, Yifan; Lei, Dong; Guan, Qingbao; Zhao, Shichang; You, Zhengwei

doi:10.1016/j.actbio.2020.01.015

Cited by 70 publications

(65 citation statements)

References 59 publications

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“…The reaction mixture was purified via precipitation in ethyl ether and vacuum-dried to yield PSeD. The porous three-dimensional scaffolds of PSeD were prepared by a modified salt fusion template method using NaCl salt particles with a size of 75–150 mM as porogen according to previous reports ( Huang et al, 2016 ; Sun et al, 2019 ; Gong et al, 2020 ; Xuan et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Numerous types of biomaterials have been used to reconstruct the orbit including autogenous materials, metallic meshes, porous polyethylene, and resorbable biomaterials ( Karsloğlu et al, 2006 ; Potter et al, 2012 ; Perez et al, 2018 ; Gao et al, 2020 ). Bioresorbable implants are becoming increasingly popular as they have several attractive properties including the ability to be easily shaped, strong mechanical integrity after resorption, and do not cause complications at the donor sites ( Xuan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Cathelicidin LL37 Promotes Osteogenic Differentiation in vitro and Bone Regeneration in vivo

Peng

Yuan

et al. 2021

Front. Bioeng. Biotechnol.

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Different types of biomaterials have been used to repair the defect of bony orbit. However, exposure and infections are still critical risks in clinical application. Biomaterials with characteristics of osteogenesis and antibiosis are needed for bone regeneration. In this study, we aimed to characterize the antimicrobial effects of cathelicidin-LL37 and to assess any impacts on osteogenic activity. Furthermore, we attempted to demonstrate the feasibility of LL37 as a potential strategy in the reconstruction of clinical bone defects. Human adipose-derived mesenchyme stem cells (hADSCs) were cultured with different concentrations of LL37 and the optimum concentration for osteogenesis was selected for further in vitro studies. We then evaluated the antibiotic properties of LL37 at the optimum osteogenic concentration. Finally, we estimated the efficiency of a PSeD/hADSCs/LL37 combined scaffold on reconstructing bone defects in the rat calvarial defect model. The osteogenic ability on hADSCs in vitro was shown to be dependent on the concentration of LL37 and reached a peak at 4 μg/ml. The optimum concentration of LL37 showed good antimicrobial properties against Escherichia coli and Staphylococcus anurans. The combination scaffold of PSeD/hADSCs/LL37 showed superior osteogenic properties compared to the PSeD/hADSCs, PSeD, and control groups scaffolds, indicating a strong bone reconstruction effect in the rat calvarial bone defect model. In Conclusion, LL37 was shown to promote osteogenic differentiation in vitro as well as antibacterial properties. The combination of PSeD/hADSCs/LL37 was advantageous in the rat calvarial defect reconstruction model, showing high potential in clinical bone regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cathelicidin LL37 Promotes Osteogenic Differentiation in vitro and Bone Regeneration in vivo

Peng

Yuan

et al. 2021

Front. Bioeng. Biotechnol.

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“…Biomaterials science recently offered an alternative approach for articular cartilage repair based on the possibility to replace the injured tissue by an artificial scaffold substitute resembling the naïve tissue, thus allowing for temporary replacement and a progressive guided self-repair. Some promising examples include the use of hydrogels (Cochis et al, 2017;Cipriani et al, 2019;Bonifacio et al, 2020;Meng et al, 2020), polymers (Pourbashir et al, 2020;Xuan et al, 2020), and composites (Gao et al, 2019;Sun et al, 2019). However, the scaffold synthesis and surgical procedures aimed at the scaffold implantation open the possibility of bacterial infection.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment

Najmi

Kumar

Scalia

et al. 2020

Front. Bioeng. Biotechnol.

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“…The main advantage of shape‐memory can again be found in its minimally invasive approach since it can be implanted in a temporary shape which is smaller in size than the final shape thereby complying better with the limited space available as well as the ability to fit irregular defects. [ 97 ]…”

Section: Biomedical Applications Of Shape‐memory Polymersmentioning

confidence: 99%

Shape‐Memory Polymers for Biomedical Applications

Delaey

Dubruel

Vlierberghe

2020

Adv Funct Materials

207

160

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One of the most promising fields for shape-memory polymers is the biomedical field. Shape-memory polymers that can be triggered by various (physiological) conditions such as temperature and moisture have been reported, as well as remotely triggerable shape-memory polymers that make use of electromagnetic fields, ultrasound, etc. These polymers have shown great promise in in vitro studies as well as in early in vivo studies. Their biocompatibility, and in some cases biodegradability, renders them excellent candidates for minimal invasive surgery and for the design of triggerable biomedical devices. The current review provides a nonexhaustive overview of recent developments realized throughout the last decade in the field of shapememory polymers serving specific biomedical applications while considering relevant triggers and biocompatible chemistries.

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Biofunctionalized chondrogenic shape-memory ternary scaffolds for efficient cell-free cartilage regeneration

Cited by 70 publications

References 59 publications

Cathelicidin LL37 Promotes Osteogenic Differentiation in vitro and Bone Regeneration in vivo

Cathelicidin LL37 Promotes Osteogenic Differentiation in vitro and Bone Regeneration in vivo

Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment

Shape‐Memory Polymers for Biomedical Applications

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