Biofunctionalization of cellulosic fibres with l-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniae

Caldeira, Estela; Pi̇şki̇n, Erhan; Granadeiro, Luiza; Silva, Filomena; Gouveia, Isabel C.

doi:10.1016/j.jbiotec.2013.10.021

Cited by 35 publications

(29 citation statements)

References 17 publications

(22 reference statements)

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“…The BNC–NH 2 membranes showed a significant reduction in bacterial cells for both E. coli and S. aureus after 24 h, whereas the BNC membranes without functionalization showed no reduction in bacterial viability . Similarly, Caldeira et al . reported nanocellulosic fibers with effective antibacterial properties obtained by the surface functionalization of 2,2,6,6‐tetramethylpiperidine‐1‐oxyl radical activated cotton cellulose fibers with l ‐cysteine.…”

Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 87%

“…Because of the enormous potential of cellulose materials, it is of great interest to develop novel functional cellulose‐based biomaterials by introducing antibacterial/antimicrobial activities through the functionalization and/or incorporation of antibacterial/antimicrobial agents. For instance, antimicrobial nanocellulose‐based materials have been obtained by the incorporation of N ‐halamine and nanocurcumin into the cellulose network and by the chemical grafting of aminoalkyl groups, 2‐benzyl‐4‐chlorophenol, l ‐cysteine, and diclofenac onto the surface of the cellulose backbone.…”

Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 99%

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Recent advances in nanocellulose for biomedical applications

Jorfi

Foster

2014

J of Applied Polymer Sci

641

368

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Nanocellulose materials have undergone rapid development in recent years as promising biomedical materials because of their excellent physical and biological properties, in particular their biocompatibility, biodegradability, and low cytotoxicity. Recently, a significant amount of research has been directed toward the fabrication of advanced cellulose nanofibers with different morphologies and functional properties. These nanocellulose fibers are widely applied in medical implants, tissue engineering, drug delivery, wound-healing, cardiovascular applications, and other medical applications. In this review, we reflect on recent advancements in the design and fabrication of advanced nanocellulose-based biomaterials (cellulose nanocrystals, bacterial nanocellulose, and cellulose nanofibrils) that are promising for biomedical applications and discuss material requirements for each application, along with the challenges that the materials might face. Finally, we give an overview on future directions of nanocellulose-based materials in the biomedical field.

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Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 87%

Section: Advanced Nanocellulose Materials For Biomedical Applicationsmentioning

confidence: 99%

Recent advances in nanocellulose for biomedical applications

Jorfi

Foster

2014

J of Applied Polymer Sci

641

368

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“…Cys-LC-LL-37 was tested against normal human dermal fibroblasts, the results of which showed no growth variation greater than 30%, which means that this antimicrobial peptide is not cytotoxic and can be used for skin contact [28]. The testing of cytotoxicity of L-Cys on connective tissue cells L 929 also showed a growth variation inferior to 30% [29]. Polypropylene showed no cytotoxicity when its extracts were blended in plasma [30], where it was shown that there was no difference on viability and cell adhesiveness of chickembryo fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Electrospinning polypropylene with an amino acid as a strategy to bind the antimicrobial peptide Cys-LC-LL-37

2017

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Hospital isolation gowns are increasingly competitive, with brands and manufacturers contesting consumer preferences. The textile materials in contact with the skin can acquire secretions and multiresistant microorganisms, causing discomfort and health risks, respectively. A new nanofibrous substratepolypropylene grafted with L-Cys-was developed with an increased crystallinity, providing its surface with-SH hooks necessary to efficiently cross-link the antimicrobial peptide Cys-LC-LL-37 in order to protect against nosocomial pathogens and their spread to community. Furthermore, this application does not require environmental control of humidity, and it is not susceptible to enzyme and microorganism degradation.

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“…For this purpose, a new strategy is proposed here: to use natural bioactive agents as potential antibacterial agents for textiles for medical applications, such as wound dressings. In this way, l ‐cysteine ( l ‐cys) was used as a model to find the best strategy to introduce active agents between the layers of chitosan (CH) and sodium alginate (ALG) because l ‐cys is an important biomolecule, which has been extensively used in pharmaceuticals, chemical synthesis, and so on . l ‐Cys can be used for the conjugation of biomolecules, and this allows it to be used for biotechnological applications …”

Section: Introductionmentioning

confidence: 99%

New biomaterial based on cotton with incorporated Biomolecules

Gomes

Mano

Queiroz

et al. 2014

J of Applied Polymer Sci

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The aim of this study was to investigate a method of embedding l‐cysteine (l‐cys), an antimicrobial agent, between layers of chitosan (CH) and sodium alginate (ALG) onto cotton samples obtained via a layer‐by‐layer electrostatic deposition technique via several embedding methods. The results show that the best way to incorporate l‐cys into the layers was the one that used the property of gelling ALG. To monitor the l‐cys embedding into the CH/ALG multilayer film, different methods were used: energy‐dispersive X‐ray spectrometry analysis to assess the presence of sulfur on the sample, Ellman's reagent method to analyze l‐cys release from the sample, and attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) to compare the ATR–FTIR spectra of the pure l‐cys and l‐cys embedded in the CH/ALG multilayer film to study the interaction between the l‐cys and the CH/ALG multilayer films. Functionalized CH/ALG cotton samples were also investigated for their antibacterial properties toward Staphylococcus aureus and Klebsiella pneumonia with the Japanese Industrial Standard method JIS L 1902:2002, and the results show an enhancement of the antibacterial effect due to the presence of l‐cys. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40519.

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Biofunctionalization of cellulosic fibres with l-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniae

Cited by 35 publications

References 17 publications

Recent advances in nanocellulose for biomedical applications

Recent advances in nanocellulose for biomedical applications

Electrospinning polypropylene with an amino acid as a strategy to bind the antimicrobial peptide Cys-LC-LL-37

New biomaterial based on cotton with incorporated Biomolecules

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