Biofilms of As(III)-oxidising bacteria: formation and activity studies for bioremediation process development

Michel, Caroline; Jean, M. M. F.; Coulon, Stéphanie; Dictor, Marie Christine; Delorme, Fabian; Morin, Dominique; Garrido, Françis

doi:10.1007/s00253-007-1169-4

Cited by 48 publications

(31 citation statements)

References 37 publications

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“…Interestingly, this site is subjected to a natural process of remediation in which Thiomonas bacterial strains play a key role by oxidizing As(III) into As(V) which co-precipitates with iron and sulfur [7], [9]. These Betaproteobacteria, defined as facultative chemolithoautotrophs, which grow optimally in mixotrophic media containing reduced inorganic sulfur compounds (RISCs) and organic supplements, are also of particular interest for their use in bioremediation [10]. Indeed, they have developed several resistance and adaptive mechanisms to cope with arsenic stress, mainly by the means of detoxification enzymes.…”

Section: Introductionmentioning

confidence: 99%

Subinhibitory Arsenite Concentrations Lead to Population Dispersal in Thiomonas sp.

et al. 2011

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Biofilms represent the most common microbial lifestyle, allowing the survival of microbial populations exposed to harsh environmental conditions. Here, we show that the biofilm development of a bacterial species belonging to the Thiomonas genus, frequently found in arsenic polluted sites and playing a key role in arsenic natural remediation, is markedly modified when exposed to subinhibitory doses of this toxic element. Indeed, arsenite [As(III)] exposure led to a considerable impact on biofilm maturation by strongly increasing the extracellular matrix synthesis and by promoting significant cell death and lysis within microcolonies. These events were followed by the development of complex 3D-biofilm structures and subsequently by the dispersal of remobilized cells observed inside the previously formed hollow voids. Our results demonstrate that this biofilm community responds to arsenite stress in a multimodal way, enhancing both survival and dispersal. Addressing this complex bacterial response to As(III) stress, which might be used by other microorganisms under various adverse conditions, may be essential to understand how Thiomonas strains persist in extreme environments.

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Section: Introductionmentioning

confidence: 99%

Subinhibitory Arsenite Concentrations Lead to Population Dispersal in Thiomonas sp.

et al. 2011

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“…In addition, in various microbial species, arsenic stress is associated with arsenite oxidase activity, biofilm formation, motility, oxidative stress or sulfur assimilation [7,8]. For example, the biofilm development by Thiomonas arsenivorans has been described as a physical barrier decreasing As(III) access to sessile cells [9]. Remarkably, some organisms such as Rhizobium sp .…”

Section: Introductionmentioning

confidence: 99%

Temporal transcriptomic response during arsenic stress in Herminiimonas arsenicoxydans

et al. 2010

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BackgroundArsenic is present in numerous ecosystems and microorganisms have developed various mechanisms to live in such hostile environments. Herminiimonas arsenicoxydans, a bacterium isolated from arsenic contaminated sludge, has acquired remarkable capabilities to cope with arsenic. In particular our previous studies have suggested the existence of a temporal induction of arsenite oxidase, a key enzyme in arsenic metabolism, in the presence of As(III).ResultsMicroarrays were designed to compare gene transcription profiles under a temporal As(III) exposure. Transcriptome kinetic analysis demonstrated the existence of two phases in arsenic response. The expression of approximatively 14% of the whole genome was significantly affected by an As(III) early stress and 4% by an As(III) late exposure. The early response was characterized by arsenic resistance, oxidative stress, chaperone synthesis and sulfur metabolism. The late response was characterized by arsenic metabolism and associated mechanisms such as phosphate transport and motility. The major metabolic changes were confirmed by chemical, transcriptional, physiological and biochemical experiments. These early and late responses were defined as general stress response and specific response to As(III), respectively.ConclusionGene expression patterns suggest that the exposure to As(III) induces an acute response to rapidly minimize the immediate effects of As(III). Upon a longer arsenic exposure, a broad metabolic response was induced. These data allowed to propose for the first time a kinetic model of the As(III) response in bacteria.

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“…SOB of the genus Thiomonas obtain energy by oxidizing sulfur, and the yeast extract contained in Cha medium promotes the growth of Thiomonas (Kelly and Wood, 2005). Bacteria of the genus Thiomonas have been isolated from activated sludge (Chen et al, 2004;Shooner et al, 1996) and mining acid drainage (Michel et al, 2007). Bioleaching by thermophilic sulfur-oxidizing microorganisms has been widely studied.…”

Section: Discussionmentioning

confidence: 99%

Middle-thermophilic sulfur-oxidizing bacteriaThiomonassp. RAN5 strain for hydrogen sulfide removal

Asano

Hirooka

Nakai

2011

Journal of the Air & Waste Management Association

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Hydrogen sulfide (H 2 S) is one of the most toxic and offensively odorous gases and is generated in anaerobic bioreactors. A middle-thermophilic sulfur-oxidizing bacterium (SOB), Thiomonas sp. strain RAN5, was isolated and applied for H 2 S removal from both artificial and anaerobically digested gas. When a bioreactor containing medium inoculated with RAN5 was aerated continuously with artificial gas (containing 100 ppm H 2 S) at 45 C for 156 hr, the H 2 S concentration in the vented gas was reduced by 99%. This was not affected by the presence of other microbes in the bioreactor. The H 2 S removal efficiency of the RAN5 bioreactor for anaerobically digested gas was greater than 99% at influent H 2 S concentrations ranging from 2 to 1800 ppm; the efficiency decreased to 90% at influent H 2 S concentrations greater than 2000 ppm. Thiomonas sp. strain RAN5 cannot survive at room temperature, and thus its leakage from a wastewater treatment plant would not damage sewage systems. These data suggest that Thiomonas sp. strain RAN5 may be a useful microorganism for H 2 S removal.Implications: The hydrogen sulfide removal efficiency of the RAN5 bioreactor was greater than 99% over the long term, for influent H 2 S concentrations of up to 2000 ppm. Removal was not affected by the presence of other microbes. Leakage of strain RAN5 would not damage sewage systems.

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Biofilms of As(III)-oxidising bacteria: formation and activity studies for bioremediation process development

Cited by 48 publications

References 37 publications

Subinhibitory Arsenite Concentrations Lead to Population Dispersal in Thiomonas sp.

Subinhibitory Arsenite Concentrations Lead to Population Dispersal in Thiomonas sp.

Temporal transcriptomic response during arsenic stress in Herminiimonas arsenicoxydans

Middle-thermophilic sulfur-oxidizing bacteriaThiomonassp. RAN5 strain for hydrogen sulfide removal

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