Biofilm sensitivity of seven<i>Streptococcus mutans</i>strains to different fluoride levels

Nassar, Hani M.; Gregory, Richard L.

doi:10.1080/20002297.2017.1328265

Cited by 24 publications

(19 citation statements)

References 36 publications

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“…The live/dead staining show that most of the GC and GCP-treated bacteria in the biofilms are alive (91–94%), which is in accordance with our finding that these compounds are not bacteriocidic. GCP that also contains fluoride, was more efficient than GC in preventing biofilm development, which can be explained by the contributing role of the fluoride ion in reducing biofilm formation of S. mutans [ 42 ]. Altogether, our data demonstrate that the GC and GCP tooth mousse prevent the adherence and biofilm development of the oral cariogenic S. mutans .…”

Section: Discussionmentioning

confidence: 99%

Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans

et al. 2021

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Background Streptococcus mutans is a common cariogenic bacterium in the oral cavity involved in plaque formation. Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has been introduced into tooth mousse to encourage remineralization of dental enamel. The aim of this research was to study the effect of tooth mousse containing CPP-ACP (GC Tooth Mousse®) or CPP-ACP with 0.2% fluoride (CPP-ACPF; GC Tooth Mousse Plus®; GCP) on S. mutans planktonic growth and biofilm formation. Methods S. mutans was cultivated in the presence of different dilutions of the tooth mousse containing CPP-ACP or CPP-ACPF, and the planktonic growth was determined by ATP viability assay and counting colony-forming units (CFUs). The resulting biofilms were examined by crystal violet staining, MTT metabolic assay, confocal laser scanning microscopy (CLSM), and scanning electron microscope (SEM). Results The CPP-ACP tooth mousse (GC) at a dilution of 5–50 mg/ml (0.5–5%) did not inhibit planktonic growth, and even increased the ATP content and the number of viable bacteria after a 24 h incubation. The same was observed for the CPP-ACPF tooth mousse (GCP), except for the higher concentrations (25 and 50 mg/ml) that led to a drop in the bacterial count. Importantly, both compounds significantly decreased S. mutans biofilm formation at dilutions as low as 1.5–3 mg/ml. 12.5 mg/ml GC and 6.25 mg/ml GCP inhibited biofilm formation by 90% after 4 h. After 24 h, the MBIC90 was 6.25 mg/ml for both. CLSM images confirmed the strong inhibitory effect GC and GCP had on biofilm formation when using 5 mg/ml tooth mousse. SEM images of those bacteria that managed to form biofilm in the presence of 5 mg/ml tooth mousse, showed alterations in the bacterial morphology, where the streptococci appear 25–30% shorter on the average than the control bacteria. Conclusion Our data show that the tooth mousse containing CPP-ACP reduces biofilm formation of the cariogenic bacterium S. mutans without killing the bacteria. The use of natural substances which inhibit biofilm development without killing the bacteria, has therapeutic benefits, especially in orthodontic pediatric patients.

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Section: Discussionmentioning

confidence: 99%

Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans

et al. 2021

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“…As a result, lactic acid secretion from glucose metabolism is hindered and consequently causes slowing down the progression of caries. Therefore, these influences of fluoride on bacteria can be advantageous out of the desensitizers tested in this study 29,30) .…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of zinc-containing desensitizer on bacterial biofilm formation and root dentin demineralization

et al. 2019

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This study compared the effect of a novel zinc containing, Caredyne Shield (CS), and a fluoroaluminocalciumsilicate-based, Nanoseal (NS) desensitizers on dentin tubule occlusion, inhibition of Streptococcus mutans (S. mutans) biofilm growth, and resistance to bacterial demineralization. Desensitizers were applied to simulated hypersensitive bovine dentin, with distilled water used as a control. S. mutans biofilms were grown on the surface of each specimen in an oral biofilm simulator. CS showed the least bacterial count and water insoluble glucan amount followed by NS. Transverse micro radiography revealed that both CS and NS showed significant reduction in mineral loss and lesion depth of the associated lesion. Scanning electron micrographs showed that the two desensitizers formed obvious depositions on the dentin surfaces, occlusion of tubules and mineral tag formation.

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“…Fluoride works by reducing bacterial tolerance to acids which will most effectively use with lower pH. 16 This research found that sodium fluoride at a concentration of 38.4 mg/ml and higher contained regrowth of S. mutans. This can be with form of adaptation from S. mutans from the use of high concentration fluoride.…”

Section: Replicationmentioning

confidence: 92%

Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro

Pradiptama

Purwanta

Notopuro

2019

BHSJ

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Introduction: Streptococcus mutans is a gram-positive coccus commonly found in the human oral cavity and is a pathogen of dental caries. S. mutans known to form biofilm in infective endocarditis heart-valve. Fluoride usage known to reduce the risk of dental caries. This study aims to analyze how usage of fluoride in inhibiting S. mutans growth.Methods: This study was an experimental study. Antibacterial activity test was performed to evaluate the minimum inhibitory concentration (MIC) using dilution method of sodium fluoride. The minimum bactericidal concentration (MBC) was determined by culturing from the previous dilution test into Chocolate Agar Plate.Results: MIC for sodium fluoride is 4,8 mg/ml and the MBC for sodium fluoride to S. mutans is 4,8 mg/ml. We found S. mutans growth in higher concentration than 19,2 mg/ml. Conclusion: S. mutans was inhibited in dilution test. Growth of the bacteria in higher concentration of sodium fluoride is explained with quasi-irreversible inhibtior effects of fluorida.

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Biofilm sensitivity of sevenStreptococcus mutansstrains to different fluoride levels

Cited by 24 publications

References 36 publications

Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans

Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans

Inhibitory effect of zinc-containing desensitizer on bacterial biofilm formation and root dentin demineralization

Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro

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