Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro

Pradiptama, Yura; Purwanta, Marijam; Notopuro, Harianto

doi:10.20473/bhsj.v2i1.13232

Cited by 11 publications

(9 citation statements)

References 16 publications

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“…To determine the inhibitory effect of the components in the glass particles, the MICs and MBCs of Zn 2+ , SiO 3 2− , and F − against S. mutans were measured via a microdilution assay. An MBC of 4.8 mg/mL (480 ppm) for NaF against S. mutans has been reported [ 33 ]. Our previous study reported an MIC of 128 ppm and MBC of 2048 ppm for F − against S. mutans [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Fabrication of pH-Responsive Zn2+-Releasing Glass Particles for Smart Antibacterial Restoratives

et al. 2022

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The on-demand release of antibacterial components due to pH variations caused by acidogenic/cariogenic bacteria is a possible design for smart antibacterial restorative materials. This study aimed to fabricate pH-responsive Zn2+-releasing glass particles and evaluate their solubilities, ion-releasing characteristics, and antibacterial properties in vitro. Three kinds of silicate-based glass particles containing different molar ratios of Zn (PG-1: 25.3; PG-2: 34.6; PG-3: 42.7 mol%) were fabricated. Each particle was immersed in a pH-adjusted medium, and the solubility and concentration of the released ions were determined. To evaluate the antibacterial effect, Streptococcus mutans was cultured in the pH-adjusted medium in the presence of each particle, and the bacterial number was counted. The solubility and concentration of Zn2+ released in the medium increased with a decrease in medium pH. PG-3 with a greater content of Zn demonstrated higher concentrations of released Zn2+ compared with PG-1 and PG-2. PG-2 exhibited bactericidal effects at pH 5.1, whereas PG-3 demonstrated bactericidal effects at pH values of 5.1 and 6.1, indicating that PG-3 was effective at inhibiting S. mutans even under slightly acidic conditions. The glass particle with 42.7 mol% Zn may be useful for developing smart antibacterial restoratives that contribute to the prevention of diseases such as caries on root surfaces with lower acid resistance.

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Section: Discussionmentioning

confidence: 99%

Fabrication of pH-Responsive Zn2+-Releasing Glass Particles for Smart Antibacterial Restoratives

et al. 2022

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“…Previous studies have concluded that glass ionomers show bacterial inhibition due to the release of fluoride [ 31 ]. Several studies have mentioned that this antibacterial effect occurs at a minimum concentration of 5000 parts per million (ppm) [ 32 ]. Other studies report that glass ionomers alone are capable of releasing fluoride between 32.6 and 17.4 ppm [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Some studies evaluated the antibacterial effect of composites modified with clay nanotubes, without evaluating the mechanical properties (microhardness, compressive strength and setting time) [ 16 , 19 ]. Other studies evaluated antimicrobial activity on different dental materials using only one strain of Streptococcus mutans as the most common bacterial strain that causes dental caries [ 1 , 6 , 7 , 9 , 16 , 32 ]. In this study, the mechanical properties and antibacterial effect on Streptococcus mutans were evaluated in glass ionomer cements modified with halloysite nanotubes pre-loaded with chlorhexidine.…”

Section: Discussionmentioning

confidence: 99%

Mechanical Properties and Antibacterial Effect on Mono-Strain of Streptococcus mutans of Orthodontic Cements Reinforced with Chlorhexidine-Modified Nanotubes

et al. 2022

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Recently, several studies have introduced nanotechnology into the area of dental materials with the aim of improving their properties. The objective of this study is to determine the antibacterial and mechanical properties of type I glass ionomers reinforced with halloysite nanotubes modified with 2% chlorhexidine at concentrations of 5% and 10% relative to the total weight of the powder used to construct each sample. Regarding antibacterial effect, 200 samples were established and distributed into four experimental groups and six control groups (4 +ve and 2 −ve), with 20 samples each. The mechanical properties were evaluated in 270 samples, assessing microhardness (30 samples), compressive strength (120 samples), and setting time (120 samples). The groups were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy, and the antibacterial activity of the ionomers was evaluated on Streptococcus mutans for 24 h. The control and positive control groups showed no antibacterial effect, while the experimental group with 5% concentration showed a zone of growth inhibition between 11.35 mm and 11.45 mm, and the group with 10% concentration showed a zone of growth inhibition between 12.50 mm and 13.20 mm. Statistical differences were observed between the experimental groups with 5% and 10% nanotubes. Regarding the mechanical properties, microhardness, and setting time, no statistical difference was found when compared with control groups, while compressive strength showed higher significant values, with ionomers modified with 10% concentration of nanotubes resulting in better compressive strength values. The incorporation of nanotubes at concentrations of 5% and 10% effectively inhibited the presence of S. mutans, particularly when the dose–response relationship was taken into account, with the advantage of maintaining and improving their mechanical properties.

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“…Hal ini sejalan dengan penelitian Lukomska-Syzmanska et al, (2016) yang membuktikan bahwa efek dari fluoride yang dilepaskan dapat menghambat pertumbuhan bakteri Lactobacillus achidopilus. 9, 15,16,17 Pada kelompok gigi premolar yang tidak menggunakan sistem adhesive two step self-etch tanpa antibakteri jumlah bakteri yang melekat lebih rendah dibandingkan kelompok gigi premolar yang tidak menggunakan sistem adhesive. Monomer dari sistem adhesive two step self-etch memberikan kelompok hidrofilik pada akhir molekul yang biasanya berupa asam, seperti hidrogen fosfat dan karboksilat.…”

Section: Pembahasanunclassified

Pengaruh Aplikasi Bonding Antibakteri Terhadap Jumlah Bakteri Lactobacillus Acidophilus Yang Melekat Pada Tumpatan Resin Komposit Bioaktif

Khadafi¹,

Nahzi²,

Wibowo³

2021

Dentin

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Background: Adhesion of Lactobacillus acidophilus can cause secondary caries and failure of tooth restorations. One of the secondary caries prevention methods is the use of bioactive composite resins which have antibacterial properties. The antibacterial effect is further enhanced by the use of bonding which has antibacterial monomers. The two-step adhesive system in the 6th generation bonding has acid monomers which have been shown to significantly inhibit bacterial growth. Objective: To determine and analyze the effect of bonding application with antibacterial content on the amount of attachment of Lactobacillus acidophilus to bioactive composite resin restoration. Methods: This study design used true experimental with a post-test only design with control group design using 39 premolars 1 tooth divided into 3 groups: the treatment group that was given bonding and the control group that did not use bonding. Results: The results of the One Way ANOVA showed a significant difference (p = 0.000). Post hoc Bonferroni test results showed a significant difference (p = 0.000) between the treatment group and the control group. Conclusion: In the use of antibacterial, non-antibacterial bonding, and without bonding, there were significant differences in the number of attachments of Lactobacillus acidophilus to the restoration material.Keywords: Antibacterial, Bioactive Composite Resin, Lactobacillus acidophilus.ABSTRAKLatar Belakang: Perlekatan Lactobacillus acidophilus dapat menyebabkan terjadinya karies sekunder dan kegagalan restorasi gigi. Salah satu metode pencegahan karies sekunder adalah dengan penggunaan resin komposit bioaktif yang memiliki sifat antibakteri. Efek antibakteri semakin ditingkatkan dengan penggunaan bonding yang memiliki monomer antibakteri. Sistem adhesive two step pada bonding generasi ke-6 memiliki monomer asam yang terbukti signifikan menghambat pertumbuhan bakteri. Tujuan: Mengetahui dan menganalisis pengaruh aplikasi bonding dengan kandungan antibakteri terhadap jumlah perlekatan bakteri Lactobacillus acidophilus pada material restorasi resin komposit bioaktif. Metode: Desain penelitian ini menggunkan penelitian eksperimental murni (true experimental) dengan rancangan post test only with control group design menggunakan 39 gigi premolar 1 dibagi menjadi 3 kelompok: kelompok perlakuan yang diberi bonding dan kelompok kontrol yang tidak menggunakan bonding. Hasil: Hasil uji statistik One Way Anova menunjukkan perbedaan bermakna (p = 0,000). Hasil uji Post hoc bonferoni menunjukkan perbedaan bermakna (p = 0,000) antara kelompok perlakuan dan kelompok kontrol. Kesimpulan: Pada penggunaan bonding antibakteri, bonding non-antibakteri, dan tanpa bonding terdapat perbedaan yang signifikan pada jumlah bakteri Lactobacillus acidophilus yang melekat pada material restorasi.Kata kunci : Antibakteri, Lactobacillus acidophilus, Resin Komposit Bioaktif.

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Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro

Cited by 11 publications

References 16 publications

Fabrication of pH-Responsive Zn2+-Releasing Glass Particles for Smart Antibacterial Restoratives

Fabrication of pH-Responsive Zn2+-Releasing Glass Particles for Smart Antibacterial Restoratives

Mechanical Properties and Antibacterial Effect on Mono-Strain of Streptococcus mutans of Orthodontic Cements Reinforced with Chlorhexidine-Modified Nanotubes

Pengaruh Aplikasi Bonding Antibakteri Terhadap Jumlah Bakteri Lactobacillus Acidophilus Yang Melekat Pada Tumpatan Resin Komposit Bioaktif

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