Biofilm Related to Dental Implants

Lee, Angie; Wang, Hom Lay

doi:10.1097/id.0b013e3181effa53

Cited by 69 publications

(53 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As implant surface infection has been repeatedly shown to be detrimental for biomaterial-host tissue integration and can lead to complications as severe as replacement surgery, there is current focus on understanding the process of bacterial adhesion to biomaterial surfaces 9 . Amongst others, Staphylococcus aureus has shown an increased likelihood to colonise and infect implants in humans and its presence is related to negative clinical prognosis [10][11][12] . Furthermore, in recent years S. aureus has demonstrated increased antibiotic resistance and as a result, novel antibacterial and anti-adhesive approaches are currently being explored in hopes of developing new strategies against biomaterial surface infection [13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

et al. 2016

View full text Add to dashboard Cite

Abstract:Despite the well-known beneficial effects of biomaterial nanopatterning on host tissue integration, the influence of controlled nanoscale topography on bacterial colonisation and infection remains unknown. Therefore, the aim of the present study was to determine the nanoscale effect of surface nanopatterning on biomaterial colonisation by S. aureus, utilising AFM nanomechanics and single-cell force spectroscopy (SCFS). Nanoindentation of S. aureus bound to planar (PL) and nanopatterned (SQ) polycarbonate (PC) surfaces suggested two distinct areas of mechanical properties, consistent with a central bacterial cell surrounded by a capsullar component. Nevertheless, no differences in elastic moduli were found between bacteria bound to PL and SQ, suggesting a minor role of nanopatterning in bacterial cell elasticity. Furthermore, SCFS demonstrated increased adhesion forces and work between S. aureus and SQ surfaces at 0s and 1s contact times. Although WLC modelling showed similarities in contour lengths for attachment to both surfaces, Poisson analysis suggests increased short-range forces for the S. aureus-SQ interactions. In the case of S. aureus-PL, long-range forces were found to not only be dominant but also repulsive in nature, which may help explain the reduced adhesion forces observed during AFM probing. In conclusion, although surface nanopatterning does not significantly influence the elasticity of attached bacterial cells, it was found to promote the early-adhesion of S. aureus cells to the biomaterial surface.

show abstract

Section: Introductionmentioning

confidence: 99%

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

et al. 2016

View full text Add to dashboard Cite

show abstract

“…ACCEPTED MANUSCRIPT 11 and cause local symptoms, such as mucositis or even carcinogenicity and mutagenicity [40][41][42][43][44].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The formation and maturation of biofilms on dental biomaterial surfaces may lead to the development of peri-implant diseases, such as peri-implant mucositis or peri-implantitis, influencing the long-term implant success [11][12][13]. With respect to such complications, oral biofilms comprise complex three-dimensional structures consisting of diverse multispecies communities as formed on oral tissues and prosthetic devices [14,15].…”

Section: Introductionmentioning

confidence: 99%

A novel coping metal material CoCrCu alloy fabricated by selective laser melting with antimicrobial and antibiofilm properties

Ren

Memarzadeh

Zhang

et al. 2016

Materials Science and Engineering: C

View full text Add to dashboard Cite

show abstract

“…Bacterial adhesion on the surface of the dental implants plays a crucial role in the onset of peri-implant diseases (HallStoodley et al, 2012;Lee and Wang, 2010). Peri-implantitis is an inflammatory gum disease involving the destruction of the tissues surrounding the implant (loss of bone support), loss of attachment of the gums and the creation of a "pocket" between the teeth and the gums.…”

Section: Introductionmentioning

confidence: 99%

“…Once gum disease has been diagnosed, immediate action is required in order to avoid biofilm progression. Biofilm establishment results from a specific sequence of events (Kolenbrander et al, 2010), starting with the attachment of early colonizers including Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii), which subsequently facilitate the adhesion of late colonizers such as Porphyromonas gingivalis (Kuboniwa et al, 2006;Lee and Wang, 2010), considered one of the most important periodontopathogenic strains (Bosshardt and Lang, 2005;Leonhardt et al, 1999). Once fully structured, biofilms are highly stable and resistant to conventional antibiotic treatments (Brouwer et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Hoyos‐Nogués

Brosel-Oliu

Abramova

et al. 2016

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Peri-implantitis, an inflammation caused by biofilm formation, constitutes a major cause of implant failure in dentistry. Thus, the detection of bacteria at the early steps of biofilm growth represents a powerful strategy to prevent implant-related infections. In this regard, antimicrobial peptides (AMPs) can be used as effective biological recognition elements to selectively detect the presence of bacteria. Thus, the aim of the present study was to combine the use of miniaturized and integrated impedimetric transducers and AMPs to obtain biosensors with high sensitivity to monitor bacterial colonization. Streptococcus sanguinis, which is one of the most prevalent strains in the onset of periodontal diseases, was used as a model of oral bacteria. To this end, a potent AMP derived from human lactoferrin was synthesized and covalently immobilized on interdigitated electrode arrays (IDEA). X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectroscopy (EIS) were employed to optimize and characterize the method of immobilization. Noteworthy, the interaction of Streptococcus sanguinis with AMP-coated sensors provoked significant changes in the impedance spectra, which were univocally associated with the presence of bacteria, proving the feasibility of our method. In this regard, the developed biosensor permits to detect the presence of bacteria at concentrations starting from 10(1) colony forming units (CFU)mL(-1) in KCl and from 10(2) CFUmL(-1) in artificial saliva. Moreover, the system was devoid of cytotoxicity for human fibroblasts. These results indicate that the proposed approach can be effective in the detection of initial stages of biofilm formation, and may be useful in the early prevention and treatment of peri-implantitis.

show abstract

Biofilm Related to Dental Implants

Cited by 69 publications

References 74 publications

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

A novel coping metal material CoCrCu alloy fabricated by selective laser melting with antimicrobial and antibiofilm properties

Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria

Contact Info

Product

Resources

About