Biofilm Formation by Drug Resistant  Enterococci Isolates Obtained from  Chronic Periodontitis Patients

Bhardwaj, Sonia Bhonchal

doi:10.7860/jcdr/2017/24472.9152

Cited by 17 publications

(29 citation statements)

References 20 publications

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“…In comparing the biofilm-forming strains (BIO+) with the non-biofilm-forming strains (BIO−), BIO+ strains were high frequency resistant than BIO− strains in clinical E. faecium isolates [39]. This observation was also declared in some other studies conducted by Sindhanai et al [40] and Bhardwaj et al [41].These results support that the biofilm forming promotes the virulence profile to microorganisms [41].…”

Section: Resultssupporting

confidence: 79%

Does subinhibitory concentrations of clinically important antibiotic induce biofilm production of Enterococcus faecium strains?

Yüksel

Karatuğ

Akçelik

2017

AMicr

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Biofilm structures are the most resistant form of active microorganisms against sanitation, disinfection, and sterilization processes. One of the specific properties of biofilm is the development of antibiotic resistance that can be up to 1,000-fold greater than planktonic cells. Enterococcus faecium is a human pathogen that causes nosocomial bacteremia and at the present time, it is well known that most of the chronic infections are biofilm-based. Recent evidence suggested that subinhibitory concentrations (sub-MICs) of antibiotics have an important role in the evolution of antibiotic resistance and induction on biofilm formation. Based on this information, we aimed to determine the effect of subinhibitory antibiotic concentrations on biofilm formation and the role of the antibiotic concentrations on the enterococcal surface protein gene (esp). To determine the impact of clinically important antibiotics on biofilm production, crystal violet assay was used. Then, the effect of sub-MICs of antibiotics on the expression of the esp gene was investigated by quantitative real-time PCR. Biofilm production assays show that MIC/2 of erythromycin (ERT; 512 μg/ml), MIC/32 of vancomycin (VAN; 16 μg/ml), MIC/64 of streptomycin (STR; 32 μg/ml), and MIC/128 of kanamycin (KAN; 4 μg/ml) values induce maximum biofilm production compared with the control. According to q-PCR results, sub-MIC values of ERT, VAN, and STR antibiotics were found to enhance esp gene expression. In addition, despite the increasing biofilm production after KAN treatment, the antibiotic was not effective on the esp expression.

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Section: Resultssupporting

confidence: 79%

Does subinhibitory concentrations of clinically important antibiotic induce biofilm production of Enterococcus faecium strains?

Yüksel

Karatuğ

Akçelik

2017

AMicr

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“…Enterococci are commensal lactic acid bacteria that are associated with human digestive tract and also are presented in prostate (Virji et al, 2015). Previous studies were showed virulence genes activities are major factors, which convert Enterococci from a harmless bacterium to virulent microorganism (Repizo et al, 2014, Radhouani et al, 2014, Bhardwaj et al, 2017. Results of present study have been showed influence of immunotherapy on virulence factors of microbial flora.…”

Section: Journal Of Infection and Molecular Biologysupporting

confidence: 56%

Immunotherapy of Prostate Cancer may Change mRNA Level of Virulence Factor Genes in E.Faecalis of Microflora

Sadeghi¹,

Talebzade²,

Altafi³

et al. 2018

J. Inf. Mol. Biol.

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“…11 Also, the widespread use of antibiotics as growth promoters in agriculture has led to the development of low-level resistance, which compromises clinical therapy during dental infections, thus favoring the appearance of multidrug resistance. 12,13 Furthermore, prolonged or repeated therapy with antimicrobial agents may lead to the development of low-level resistance that compromises the effectiveness of antibiotic treatment, which may not be detected by routine susceptibility laboratory tests. Therefore, it is possible that more virulent multidrug antibiotic-resistant bacteria (MARB) could form as a result of overall bacterial combinations.…”

Section: Beginning and Mechanisms Of Antibiotic Bacterial Resistancementioning

confidence: 99%

“…It was reported that biofilms are resistant to antibiotics at concentrations 10‐1000 times greater than those needed to kill free‐living or planktonic cells . Also, the widespread use of antibiotics as growth promoters in agriculture has led to the development of low‐level resistance, which compromises clinical therapy during dental infections, thus favoring the appearance of multidrug resistance . Furthermore, prolonged or repeated therapy with antimicrobial agents may lead to the development of low‐level resistance that compromises the effectiveness of antibiotic treatment, which may not be detected by routine susceptibility laboratory tests.…”

Section: Introductionmentioning

confidence: 99%

Prevention of infective endocarditis and bacterial resistance to antibiotics: A brief review

Loyola-Rodríguez

Miranda

Loyola-Leyva

et al. 2019

Special Care in Dentistry

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The purpose of this statement is to debate the recommendations of the American Heart Association (AHA) for the prevention of infective endocarditis through an antibiotic prophylaxis protocol and its relation with bacterial resistance to antibiotics. Since dental infections involve biofilms that include several bacterial species (Gram‐negative and Gram‐positive), it is essential, from the dental point of view, to consider the frequency, magnitude, and duration of bacteremia associated with active dental infections before applying antibiotic prophylaxis. The actual guidelines for antibiotic prophylaxis should be revised according to recent evidence of bacterial resistance. Amoxicillin/clavulanic acid and moxifloxacin should be considered due to their effectiveness against bacteria associated with oral, GU, and GI infections and the low rates of antibiotic resistance associated with these antibiotics, instead of the actual protocol, which includes amoxicillin (2 g) or clindamycin (600 mg) administered an hour before the dental procedures. The breaking point to test the antibiotic bacterial resistance (ABR) had a wide range in the different studies that were analyzed, which could explain the widely varied ABR percentages reported for the various antibiotics used for antibiotic prophylaxis.

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Biofilm Formation by Drug Resistant Enterococci Isolates Obtained from Chronic Periodontitis Patients

Cited by 17 publications

References 20 publications

Does subinhibitory concentrations of clinically important antibiotic induce biofilm production of Enterococcus faecium strains?

Does subinhibitory concentrations of clinically important antibiotic induce biofilm production of Enterococcus faecium strains?

Immunotherapy of Prostate Cancer may Change mRNA Level of Virulence Factor Genes in E.Faecalis of Microflora

Prevention of infective endocarditis and bacterial resistance to antibiotics: A brief review

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