Biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in Argentina

Abstract

Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to β-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.

Coagulase Negative Staphylococci as an emerging cause of bovine mastitis: prevalence, antimicrobial resistance and biofilm formation

“…While, S. xylosus was the species with the highest ability to form biofilm. Srednik et al (2017) differently reported that S. chromogenes and S. sciuri were the most species with biofilm formation incidence. S. haemolyticus and S. devriesei isolates formed significantly more strong biofilms than other CNS.…”

Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland

“…Biofilms were formed in 96-well microtiter plates as described by Srednik et al [ 32 ]. The colonies were suspended in fresh Luria–Bertani (LB) broth to 0.5 McFarland standards, and 200 μL was deposited on the microtiter plates in triplicate.…”

Prevalence and Genetic Basis of Antimicrobial Resistance in Non-aureus Staphylococci Isolated from Canadian Dairy Herds

“…Whereas recent reports illustrate that NAS may harbor resistance determinants (Srednik et al, 2017b ; Wipf et al, 2017 ) including genes considered potential public health hazards (Srednik et al, 2017a ), the prevalence of AMR and respective resistance determinants in NAS isolated from dairy herds remains unknown. It is noteworthy that the vast majority of studies designed to estimate the prevalence of AMR in NAS isolated from dairy herds are regional and limit screening of resistance determinants to resistant isolates, usually by PCR or a similar approach.…”